{"id":{"repo_id":"vcu","oai_identifier":"oai:scholarscompass.vcu.edu:etd-1777"},"canonical_url":"https://search.dev.ndltd.org/etd/vcu/oai:scholarscompass.vcu.edu:etd-1777","repository":{"repo_id":"vcu","name":"Virginia Commonwealth University","base_url":"https://scholarscompass.vcu.edu/do/oai/"},"display":{"title":"Functional Consequences of Matrix Metalloproteinase-1 Over-Expression in Human Gliomas","abstract":"Malignant brain tumors are among the deadliest of human cancers. Despit recent advancements in conventional therapies, glioblastomas remain incurable, largel y due to their ability to invade surrounding tissue. Matrix metalloproteinases are thought to contribute to the invaseive phenotype of human gliomas. Absent in normal brain, matrix metalloproteinase-1 (MMP-1) has been shown to be present in gliomas, and in particular in glioblastoma multiforme (GBM). To begin to examine the role of MMP-1 in these tumore, two human glioma cell lines were stably transfected with MMP-1 cDNA. Confirmation of MMP-1 over-expression in these cells was achieved through real-time PCR and Western blot analysis. The functional consequences of MMP-1 over-expression were analyzed using a collagen type-I invasion assay along with clonogenic and ATP viability assays. Data presented demonstrate that MMP-1 over-expressing cells were more invasive in both cell types and interestingly more clonogenic in on of the glioma cell lines, supporting a possible role for MMP-1 in glioma growth and invasion.","abstract_html":"Malignant brain tumors are among the deadliest of human cancers. Despit recent advancements in conventional therapies, glioblastomas remain incurable, largel y due to their ability to invade surrounding tissue. Matrix metalloproteinases are thought to contribute to the invaseive phenotype of human gliomas. Absent in normal brain, matrix metalloproteinase-1 (MMP-1) has been shown to be present in gliomas, and in particular in glioblastoma multiforme (GBM). To begin to examine the role of MMP-1 in these tumore, two human glioma cell lines were stably transfected with MMP-1 cDNA. Confirmation of MMP-1 over-expression in these cells was achieved through real-time PCR and Western blot analysis. The functional consequences of MMP-1 over-expression were analyzed using a collagen type-I invasion assay along with clonogenic and ATP viability assays. Data presented demonstrate that MMP-1 over-expressing cells were more invasive in both cell types and interestingly more clonogenic in on of the glioma cell lines, supporting a possible role for MMP-1 in glioma growth and invasion.","abstract_has_math":false,"creators":["Mullet, Emily"],"institution":null,"degree_name":"Master of Science","degree_level":"Thesis","degree_discipline":"Physiology","degree_department":null,"school":null,"contributors":["Dr. Helen L. Fillmore"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2006,"date_issued":"2006-01-01T08:00:00Z","date_published":"2006-01-01T08:00:00Z","updated_at":"2026-07-24T05:54:21Z","subjects":["MMP-1","GBM","matrix metalloproteinases","brain tumor","Life Sciences","Physiology"],"languages":[],"rights":["© The Author"],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["https://scholarscompass.vcu.edu/etd/778"],"render_values":[{"text":"https://scholarscompass.vcu.edu/etd/778","href":"https://scholarscompass.vcu.edu/etd/778","code":true}]}]},"links":{"outbound_url":"https://doi.org/10.25772/XRD9-XB50","outbound_label":"DOI","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Dr. Helen L. 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Despit recent advancements in conventional therapies, glioblastomas remain incurable, largel y due to their ability to invade surrounding tissue. Matrix metalloproteinases are thought to contribute to the invaseive phenotype of human gliomas. Absent in normal brain, matrix metalloproteinase-1 (MMP-1) has been shown to be present in gliomas, and in particular in glioblastoma multiforme (GBM). To begin to examine the role of MMP-1 in these tumore, two human glioma cell lines were stably transfected with MMP-1 cDNA. Confirmation of MMP-1 over-expression in these cells was achieved through real-time PCR and Western blot analysis. The functional consequences of MMP-1 over-expression were analyzed using a collagen type-I invasion assay along with clonogenic and ATP viability assays. Data presented demonstrate that MMP-1 over-expressing cells were more invasive in both cell types and interestingly more clonogenic in on of the glioma cell lines, supporting a possible role for MMP-1 in glioma growth and invasion."]},{"key":"dc:title","label":"Title","values":["Functional Consequences of Matrix Metalloproteinase-1 Over-Expression in Human Gliomas"]}]}],"canonical_facts":{"dc:contributor":["Dr. Helen L. Fillmore"],"dc:creator":["Mullet, Emily"],"dc:date.available":["2014-07-09T07:00:00Z"],"dc:description.abstract":["Malignant brain tumors are among the deadliest of human cancers. Despit recent advancements in conventional therapies, glioblastomas remain incurable, largel y due to their ability to invade surrounding tissue. Matrix metalloproteinases are thought to contribute to the invaseive phenotype of human gliomas. Absent in normal brain, matrix metalloproteinase-1 (MMP-1) has been shown to be present in gliomas, and in particular in glioblastoma multiforme (GBM). To begin to examine the role of MMP-1 in these tumore, two human glioma cell lines were stably transfected with MMP-1 cDNA. Confirmation of MMP-1 over-expression in these cells was achieved through real-time PCR and Western blot analysis. The functional consequences of MMP-1 over-expression were analyzed using a collagen type-I invasion assay along with clonogenic and ATP viability assays. Data presented demonstrate that MMP-1 over-expressing cells were more invasive in both cell types and interestingly more clonogenic in on of the glioma cell lines, supporting a possible role for MMP-1 in glioma growth and invasion."],"dc:identifier":["https://doi.org/10.25772/XRD9-XB50","https://scholarscompass.vcu.edu/etd/778"],"dc:rights":["© The Author"],"dc:subject":["MMP-1","GBM","matrix metalloproteinases","brain tumor","Life Sciences","Physiology"],"dc:title":["Functional Consequences of Matrix Metalloproteinase-1 Over-Expression in Human Gliomas"],"thesis:degree_discipline":["Physiology"],"thesis:degree_level":["Thesis"],"thesis:degree_name":["Master of Science"]},"updated_at":"2026-07-24T05:54:21Z"}