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University of Illinois at Urbana-Champaign
Mass Spectrometry and Thiotemplate Assembly: Unique Insights Into the Production of Natural Products on >100 kDa Enzymes
Abstract
dc:description"This work further extends initial studies into using FTMS to examine NRP/PK synthetases and provides the most detailed insights into pyochelin, yersiniabactin and FK520 biosynthesis achieved to date. This study also represents the first observation of multiple covalently modified active site peptides in parallel using robust digestion protocols. It is hoped that further refinement of this mass spectrometry-based platform will bolster NRP/PK reengineering efforts towards the design of ""unnatural"" natural products that combat modern antibiotic-resistant pathogens."
Degree
thesis:*- Name thesis:degree_name
- Ph.D.
- Level thesis:degree_level
- Dissertation
- Discipline thesis:degree_discipline
- Chemistry
- Grantor
- University of Illinois at Urbana-Champaign
- Year dc:date
- 2015
Author and committee
dc:creator, dc:contributor.*- Author dc:creator
-
- McLoughlin, Shaun Michael
- Contributors dc:contributor
-
- Kelleher, Neil L.
Subjects
dc:subject × 1Rights
- Language dc:language
- eng
Identifiers
dc:identifier.*- Identifier
- (MiAaPQ)AAI3199084
- OAI identifier oai:identifier
- oai:www.ideals.illinois.edu:2142/84195