{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/84195"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/84195","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Mass Spectrometry and Thiotemplate Assembly: Unique Insights Into the Production of Natural Products on >100 kDa Enzymes","abstract":"\"This work further extends initial studies into using FTMS to examine NRP/PK synthetases and provides the most detailed insights into pyochelin, yersiniabactin and FK520 biosynthesis achieved to date. This study also represents the first observation of multiple covalently modified active site peptides in parallel using robust digestion protocols. It is hoped that further refinement of this mass spectrometry-based platform will bolster NRP/PK reengineering efforts towards the design of \"\"unnatural\"\" natural products that combat modern antibiotic-resistant pathogens.\"","abstract_html":"&quot;This work further extends initial studies into using FTMS to examine NRP/PK synthetases and provides the most detailed insights into pyochelin, yersiniabactin and FK520 biosynthesis achieved to date. This study also represents the first observation of multiple covalently modified active site peptides in parallel using robust digestion protocols. It is hoped that further refinement of this mass spectrometry-based platform will bolster NRP/PK reengineering efforts towards the design of &quot;&quot;unnatural&quot;&quot; natural products that combat modern antibiotic-resistant pathogens.&quot;","abstract_has_math":false,"creators":["McLoughlin, Shaun Michael"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Chemistry","degree_department":null,"school":null,"contributors":["Kelleher, Neil L."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2015,"date_issued":"2015-09-25T22:13:25Z","date_published":"2015-09-25T22:13:25Z","updated_at":"2026-07-22T22:26:22Z","subjects":["Chemistry, Biochemistry"],"languages":["eng"],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["(MiAaPQ)AAI3199084"],"render_values":[{"text":"(MiAaPQ)AAI3199084","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/84195","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Kelleher, Neil L."]},{"key":"dc:creator","label":"Author","values":["McLoughlin, Shaun Michael"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2015-09-25T22:13:25Z","10000-01-01","2005"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Chemistry"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Chemistry, Biochemistry"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/84195","(MiAaPQ)AAI3199084"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["\"This work further extends initial studies into using FTMS to examine NRP/PK synthetases and provides the most detailed insights into pyochelin, yersiniabactin and FK520 biosynthesis achieved to date. This study also represents the first observation of multiple covalently modified active site peptides in parallel using robust digestion protocols. It is hoped that further refinement of this mass spectrometry-based platform will bolster NRP/PK reengineering efforts towards the design of \"\"unnatural\"\" natural products that combat modern antibiotic-resistant pathogens.\"","Made available in DSpace on 2015-09-25T22:13:25Z (GMT). No. of bitstreams: 2 license.txt: 4848 bytes, checksum: 96035ab3f5e1c23cc7138a224ce498bd (MD5) 3199084.pdf: 5955396 bytes, checksum: 3526f353a061cf0eaa55c6d7971dc483 (MD5) Previous issue date: 2005","Embargo set by: Seth Robbins for item 85476 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","U of I Only","190 p.","Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2005."]},{"key":"dc:title","label":"Title","values":["Mass Spectrometry and Thiotemplate Assembly: Unique Insights Into the Production of Natural Products on >100 kDa Enzymes"]}]}],"canonical_facts":{"dc:contributor":["Kelleher, Neil L."],"dc:creator":["McLoughlin, Shaun Michael"],"dc:date":["2015-09-25T22:13:25Z","10000-01-01","2005"],"dc:description":["\"This work further extends initial studies into using FTMS to examine NRP/PK synthetases and provides the most detailed insights into pyochelin, yersiniabactin and FK520 biosynthesis achieved to date. This study also represents the first observation of multiple covalently modified active site peptides in parallel using robust digestion protocols. It is hoped that further refinement of this mass spectrometry-based platform will bolster NRP/PK reengineering efforts towards the design of \"\"unnatural\"\" natural products that combat modern antibiotic-resistant pathogens.\"","Made available in DSpace on 2015-09-25T22:13:25Z (GMT). No. of bitstreams: 2 license.txt: 4848 bytes, checksum: 96035ab3f5e1c23cc7138a224ce498bd (MD5) 3199084.pdf: 5955396 bytes, checksum: 3526f353a061cf0eaa55c6d7971dc483 (MD5) Previous issue date: 2005","Embargo set by: Seth Robbins for item 85476 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","U of I Only","190 p.","Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2005."],"dc:identifier":["http://hdl.handle.net/2142/84195","(MiAaPQ)AAI3199084"],"dc:language":["eng"],"dc:subject":["Chemistry, Biochemistry"],"dc:title":["Mass Spectrometry and Thiotemplate Assembly: Unique Insights Into the Production of Natural Products on >100 kDa Enzymes"],"dc:type":["text"],"thesis:degree_discipline":["Chemistry"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:26:22Z"}