QUB-1475: A novel bioactive peptide from the skin secretion of frog Hylarana Guentheri

As antimicrobial resistance has become a threat towards human health for decades, the demand for the development of an alternative, broad-spectrum antimicrobial agent has turned into increasingly essential. As a consequence, the emergence of the antimicrobial peptide (AMP) derived from the skin secretion of amphibians has promoted the development of novel potential clinical therapeutics. Therefore, a novel potential bioactive peptide QUB-1475 derived from the frog species Hylarana guentheri was chosen here to get thoroughly studied. <br/>In this research, the novel temporin-like peptide, with the sequence: FLQHIIGALGFIF-NH2, was discovered in the skin secretion of Hylarana guentheri. This thesis aimed to isolate and characterise the structure of the novel peptide QUB-1475 through “shotgun” molecular cloning, reverse-phase High-Performance Liquid Chromatography (RP-HPLC) and Matrix Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry (MALDI-TOF MS) analysis. Then the peptide was chemically-synthesised and evaluated through a series of bioactivity assays.<br/>This peptide displayed no antimicrobial activity on S. aureus, E. coli and C. albicans conducted in this research. The IC50 values of the peptide towards NCI-H23 cell line, PC3 cell line and U-251 MG cell line are 26.51 μM, 10.03 μM, and 44.78 μM respectively. Moreover, it should be emphasised that the novel peptide presented haemolytic rate of 18.95% at the concentration of 512 μM. <br/><br/><i>Thesis embargoed until 30th September 2024 </i>

Name dc:type.qualificationname

Master of Philosophy

Level dc:type.qualificationlevel

Masters Thesis

Grantor dc:publisher.institution

Queen's University Belfast

Year dc:date.issued

2019

Author dc:creator

• Zheng, Jiewen

Advisors dc:contributor.advisor

• Chen, Tianbao
• Zhou, Mei
• Wang, Lei
• Ma, Chengbang

Language dc:language

eng

Identifier

oai:pure.qub.ac.uk/portal:studenttheses/a6857c73-6f15-42b4-8ded-30702faedf0f

OAI identifier oai:identifier

oai:pure.qub.ac.uk/portal:studenttheses/a6857c73-6f15-42b4-8ded-30702faedf0f