{"id":{"repo_id":"qu-belfast","oai_identifier":"oai:pure.qub.ac.uk/portal:studenttheses/a6857c73-6f15-42b4-8ded-30702faedf0f"},"canonical_url":"https://search.dev.ndltd.org/etd/qu-belfast/oai:pure.qub.ac.uk/portal:studenttheses/a6857c73-6f15-42b4-8ded-30702faedf0f","repository":{"repo_id":"qu-belfast","name":"Queen's University Belfast","base_url":"https://pureadmin.qub.ac.uk/ws/oai"},"display":{"title":"QUB-1475: A novel bioactive peptide from the skin secretion of frog Hylarana Guentheri","abstract":"As antimicrobial resistance has become a threat towards human health for decades, the demand for the development of an alternative, broad-spectrum antimicrobial agent has turned into increasingly essential. As a consequence, the emergence of the antimicrobial peptide (AMP) derived from the skin secretion of amphibians has promoted the development of novel potential clinical therapeutics. Therefore, a novel potential bioactive peptide QUB-1475 derived from the frog species Hylarana guentheri was chosen here to get thoroughly studied. <br/>In this research, the novel temporin-like peptide, with the sequence: FLQHIIGALGFIF-NH2, was discovered in the skin secretion of Hylarana guentheri. This thesis aimed to isolate and characterise the structure of the novel peptide QUB-1475 through “shotgun” molecular cloning, reverse-phase High-Performance Liquid Chromatography (RP-HPLC) and Matrix Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry (MALDI-TOF MS) analysis. Then the peptide was chemically-synthesised and evaluated through a series of bioactivity assays.<br/>This peptide displayed no antimicrobial activity on S. aureus, E. coli and C. albicans conducted in this research. The IC50 values of the peptide towards NCI-H23 cell line, PC3 cell line and U-251 MG cell line are 26.51 μM, 10.03 μM, and 44.78 μM respectively. Moreover, it should be emphasised that the novel peptide presented haemolytic rate of 18.95% at the concentration of 512 μM. <br/><br/><i>Thesis embargoed until 30th September 2024 </i>","abstract_html":"As antimicrobial resistance has become a threat towards human health for decades, the demand for the development of an alternative, broad-spectrum antimicrobial agent has turned into increasingly essential. As a consequence, the emergence of the antimicrobial peptide (AMP) derived from the skin secretion of amphibians has promoted the development of novel potential clinical therapeutics. Therefore, a novel potential bioactive peptide QUB-1475 derived from the frog species Hylarana guentheri was chosen here to get thoroughly studied. &lt;br/&gt;In this research, the novel temporin-like peptide, with the sequence: FLQHIIGALGFIF-NH2, was discovered in the skin secretion of Hylarana guentheri. This thesis aimed to isolate and characterise the structure of the novel peptide QUB-1475 through “shotgun” molecular cloning, reverse-phase High-Performance Liquid Chromatography (RP-HPLC) and Matrix Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry (MALDI-TOF MS) analysis. Then the peptide was chemically-synthesised and evaluated through a series of bioactivity assays.&lt;br/&gt;This peptide displayed no antimicrobial activity on S. aureus, E. coli and C. albicans conducted in this research. The IC50 values of the peptide towards NCI-H23 cell line, PC3 cell line and U-251 MG cell line are 26.51 μM, 10.03 μM, and 44.78 μM respectively. Moreover, it should be emphasised that the novel peptide presented haemolytic rate of 18.95% at the concentration of 512 μM. &lt;br/&gt;&lt;br/&gt;&lt;i&gt;Thesis embargoed until 30th September 2024 &lt;/i&gt;","abstract_has_math":false,"creators":["Zheng, Jiewen"],"institution":"Queen's University Belfast","degree_name":"Master of Philosophy","degree_level":"Masters Thesis","degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":["Chen, Tianbao","Zhou, Mei","Wang, Lei","Ma, Chengbang"],"committee_chairs":[],"committee_members":[],"year":2019,"date_issued":"2019-12","date_published":"2019-12","updated_at":"2026-07-24T03:55:05Z","subjects":[],"languages":["eng"],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["oai:pure.qub.ac.uk/portal:studenttheses/a6857c73-6f15-42b4-8ded-30702faedf0f"],"render_values":[{"text":"oai:pure.qub.ac.uk/portal:studenttheses/a6857c73-6f15-42b4-8ded-30702faedf0f","href":null,"code":true}]}]},"links":{"outbound_url":"https://pure.qub.ac.uk/en/studentTheses/a6857c73-6f15-42b4-8ded-30702faedf0f","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Chen, Tianbao","Zhou, Mei","Wang, Lei","Ma, Chengbang"]},{"key":"dc:creator","label":"Author","values":["Zheng, Jiewen"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2019-12"]},{"key":"dc:date.issued","label":"Date","values":["2019-12"]},{"key":"dc:publisher.department","label":"Dc Publisher Department","values":["School of Pharmacy"]},{"key":"dc:publisher.institution","label":"Dc Publisher Institution","values":["Queen's University Belfast"]},{"key":"dc:relation.isreferencedby","label":"Dc Relation Isreferencedby","values":["https://pure.qub.ac.uk/en/studentTheses/a6857c73-6f15-42b4-8ded-30702faedf0f"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]},{"key":"dc:type.qualificationlevel","label":"Dc Type Qualificationlevel","values":["Masters Thesis"]},{"key":"dc:type.qualificationname","label":"Dc Type Qualificationname","values":["Master of Philosophy"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]},{"key":"dc:rights.embargodate","label":"Dc Rights Embargodate","values":["2024-09-30"]},{"key":"dc:rights.embargoreason","label":"Dc Rights Embargoreason","values":["/dk/atira/pure/core/document/studentthesisembargoreason/publicationissues"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["oai:pure.qub.ac.uk/portal:studenttheses/a6857c73-6f15-42b4-8ded-30702faedf0f","https://pure.qub.ac.uk/en/studentTheses/a6857c73-6f15-42b4-8ded-30702faedf0f"]},{"key":"dc:identifier.uri","label":"Identifier URI","values":["https://pure.qub.ac.uk/files/183470395/Jiewen_Zheng_Dissertation.pdf"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["As antimicrobial resistance has become a threat towards human health for decades, the demand for the development of an alternative, broad-spectrum antimicrobial agent has turned into increasingly essential. As a consequence, the emergence of the antimicrobial peptide (AMP) derived from the skin secretion of amphibians has promoted the development of novel potential clinical therapeutics. Therefore, a novel potential bioactive peptide QUB-1475 derived from the frog species Hylarana guentheri was chosen here to get thoroughly studied. <br/>In this research, the novel temporin-like peptide, with the sequence: FLQHIIGALGFIF-NH2, was discovered in the skin secretion of Hylarana guentheri. This thesis aimed to isolate and characterise the structure of the novel peptide QUB-1475 through “shotgun” molecular cloning, reverse-phase High-Performance Liquid Chromatography (RP-HPLC) and Matrix Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry (MALDI-TOF MS) analysis. Then the peptide was chemically-synthesised and evaluated through a series of bioactivity assays.<br/>This peptide displayed no antimicrobial activity on S. aureus, E. coli and C. albicans conducted in this research. The IC50 values of the peptide towards NCI-H23 cell line, PC3 cell line and U-251 MG cell line are 26.51 μM, 10.03 μM, and 44.78 μM respectively. Moreover, it should be emphasised that the novel peptide presented haemolytic rate of 18.95% at the concentration of 512 μM. <br/><br/><i>Thesis embargoed until 30th September 2024 </i>"]},{"key":"dc:title","label":"Title","values":["QUB-1475: A novel bioactive peptide from the skin secretion of frog Hylarana Guentheri"]}]}],"canonical_facts":{"dc:contributor.advisor":["Chen, Tianbao","Zhou, Mei","Wang, Lei","Ma, Chengbang"],"dc:creator":["Zheng, Jiewen"],"dc:date":["2019-12"],"dc:date.issued":["2019-12"],"dc:description.abstract":["As antimicrobial resistance has become a threat towards human health for decades, the demand for the development of an alternative, broad-spectrum antimicrobial agent has turned into increasingly essential. 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The IC50 values of the peptide towards NCI-H23 cell line, PC3 cell line and U-251 MG cell line are 26.51 μM, 10.03 μM, and 44.78 μM respectively. 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