QUB-1957: a bioactive peptide from the frog skin secretion of Hylarana latouchii

The skin secretions of amphibians are significant for amphibians’ daily protection, especially the antimicrobial peptides. In this study, a cDNA library from lyophilised skin secretion of Hylarana latouchii was constructed, and molecular cloning of biosynthetic precursor-encoding cDNAs of QUB-1957 was performed. Subsequently, QUB-1957 was synthesised by Solid Phase Peptide Synthesizer. In the antimicrobial assays, the MIC of QUB-1957 against Gram-positive bacteria Staphylococcus aureus, Gram-negative bacteria Escherichia coli and yeast Candida albicans are 8 μM, 16 μM and 16 μM, respectively. Unfortunately, QUB-1957 also exhibits a certain degree of haemolytic rate, the HC50 is 47.82 μM. Besides, QUB-1957 exhibits no significant effect against PC3 (human prostate cancer cell line), NCI-H157 (human non-small cell lung cancer cell line), MCF-7 (human breast cancer cell line) and U251MG (human neuronal glioblastoma cell line). These characteristics of QUB-1957 indicate that QUB-1957 is a promising antimicrobial agent with a broad spectrum that worth further study. Hopefully, the structure QUB-1957 could be researched for novel antimicrobial agent development.

Name dc:type.qualificationname

Master of Philosophy

Level dc:type.qualificationlevel

Masters Thesis

Grantor dc:publisher.institution

Queen's University Belfast

Year dc:date.issued

2018

Author dc:creator

• Yang, Ruoning

Advisors dc:contributor.advisor

• Chen, Tianbao
• Zhou, Mei
• Wang, Lei
• Shaw, Christopher

Language dc:language

eng

Identifier

oai:pure.qub.ac.uk/portal:studenttheses/8e038084-7079-4243-a461-6e4533836645

OAI identifier oai:identifier

oai:pure.qub.ac.uk/portal:studenttheses/8e038084-7079-4243-a461-6e4533836645