{"id":{"repo_id":"qu-belfast","oai_identifier":"oai:pure.qub.ac.uk/portal:studenttheses/8e038084-7079-4243-a461-6e4533836645"},"canonical_url":"https://search.dev.ndltd.org/etd/qu-belfast/oai:pure.qub.ac.uk/portal:studenttheses/8e038084-7079-4243-a461-6e4533836645","repository":{"repo_id":"qu-belfast","name":"Queen's University Belfast","base_url":"https://pureadmin.qub.ac.uk/ws/oai"},"display":{"title":"QUB-1957: a bioactive peptide from the frog skin secretion of Hylarana latouchii","abstract":"The skin secretions of amphibians are significant for amphibians’ daily protection, especially the antimicrobial peptides. In this study, a cDNA library from lyophilised skin secretion of Hylarana latouchii was constructed, and molecular cloning of biosynthetic precursor-encoding cDNAs of QUB-1957 was performed. Subsequently, QUB-1957 was synthesised by Solid Phase Peptide Synthesizer. In the antimicrobial assays, the MIC of QUB-1957 against Gram-positive bacteria Staphylococcus aureus, Gram-negative bacteria Escherichia coli and yeast Candida albicans are 8 μM, 16 μM and 16 μM, respectively. Unfortunately, QUB-1957 also exhibits a certain degree of haemolytic rate, the HC50 is 47.82 μM. Besides, QUB-1957 exhibits no significant effect against PC3 (human prostate cancer cell line), NCI-H157 (human non-small cell lung cancer cell line), MCF-7 (human breast cancer cell line) and U251MG (human neuronal glioblastoma cell line). These characteristics of QUB-1957 indicate that QUB-1957 is a promising antimicrobial agent with a broad spectrum that worth further study. Hopefully, the structure QUB-1957 could be researched for novel antimicrobial agent development.","abstract_html":"The skin secretions of amphibians are significant for amphibians’ daily protection, especially the antimicrobial peptides. In this study, a cDNA library from lyophilised skin secretion of Hylarana latouchii was constructed, and molecular cloning of biosynthetic precursor-encoding cDNAs of QUB-1957 was performed. Subsequently, QUB-1957 was synthesised by Solid Phase Peptide Synthesizer. In the antimicrobial assays, the MIC of QUB-1957 against Gram-positive bacteria Staphylococcus aureus, Gram-negative bacteria Escherichia coli and yeast Candida albicans are 8 μM, 16 μM and 16 μM, respectively. Unfortunately, QUB-1957 also exhibits a certain degree of haemolytic rate, the HC50 is 47.82 μM. Besides, QUB-1957 exhibits no significant effect against PC3 (human prostate cancer cell line), NCI-H157 (human non-small cell lung cancer cell line), MCF-7 (human breast cancer cell line) and U251MG (human neuronal glioblastoma cell line). These characteristics of QUB-1957 indicate that QUB-1957 is a promising antimicrobial agent with a broad spectrum that worth further study. Hopefully, the structure QUB-1957 could be researched for novel antimicrobial agent development.","abstract_has_math":false,"creators":["Yang, Ruoning"],"institution":"Queen's University Belfast","degree_name":"Master of Philosophy","degree_level":"Masters Thesis","degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":["Chen, Tianbao","Zhou, Mei","Wang, Lei","Shaw, Christopher"],"committee_chairs":[],"committee_members":[],"year":2018,"date_issued":"2018-7","date_published":"2018-7","updated_at":"2026-07-24T03:54:53Z","subjects":[],"languages":["eng"],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["oai:pure.qub.ac.uk/portal:studenttheses/8e038084-7079-4243-a461-6e4533836645"],"render_values":[{"text":"oai:pure.qub.ac.uk/portal:studenttheses/8e038084-7079-4243-a461-6e4533836645","href":null,"code":true}]}]},"links":{"outbound_url":"https://pure.qub.ac.uk/en/studentTheses/8e038084-7079-4243-a461-6e4533836645","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Chen, Tianbao","Zhou, Mei","Wang, Lei","Shaw, Christopher"]},{"key":"dc:creator","label":"Author","values":["Yang, Ruoning"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2018-7"]},{"key":"dc:date.issued","label":"Date","values":["2018-7"]},{"key":"dc:publisher.department","label":"Dc Publisher Department","values":["School of Pharmacy"]},{"key":"dc:publisher.institution","label":"Dc Publisher Institution","values":["Queen's University Belfast"]},{"key":"dc:relation.isreferencedby","label":"Dc Relation Isreferencedby","values":["https://pure.qub.ac.uk/en/studentTheses/8e038084-7079-4243-a461-6e4533836645"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]},{"key":"dc:type.qualificationlevel","label":"Dc Type Qualificationlevel","values":["Masters Thesis"]},{"key":"dc:type.qualificationname","label":"Dc Type Qualificationname","values":["Master of Philosophy"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]},{"key":"dc:rights.embargodate","label":"Dc Rights Embargodate","values":["2023-09-30"]},{"key":"dc:rights.embargoreason","label":"Dc Rights Embargoreason","values":["/dk/atira/pure/core/document/studentthesisembargoreason/publicationissues"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["oai:pure.qub.ac.uk/portal:studenttheses/8e038084-7079-4243-a461-6e4533836645","https://pure.qub.ac.uk/en/studentTheses/8e038084-7079-4243-a461-6e4533836645"]},{"key":"dc:identifier.uri","label":"Identifier URI","values":["https://pure.qub.ac.uk/files/157057950/_6.pdf"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["The skin secretions of amphibians are significant for amphibians’ daily protection, especially the antimicrobial peptides. In this study, a cDNA library from lyophilised skin secretion of Hylarana latouchii was constructed, and molecular cloning of biosynthetic precursor-encoding cDNAs of QUB-1957 was performed. Subsequently, QUB-1957 was synthesised by Solid Phase Peptide Synthesizer. In the antimicrobial assays, the MIC of QUB-1957 against Gram-positive bacteria Staphylococcus aureus, Gram-negative bacteria Escherichia coli and yeast Candida albicans are 8 μM, 16 μM and 16 μM, respectively. Unfortunately, QUB-1957 also exhibits a certain degree of haemolytic rate, the HC50 is 47.82 μM. Besides, QUB-1957 exhibits no significant effect against PC3 (human prostate cancer cell line), NCI-H157 (human non-small cell lung cancer cell line), MCF-7 (human breast cancer cell line) and U251MG (human neuronal glioblastoma cell line). These characteristics of QUB-1957 indicate that QUB-1957 is a promising antimicrobial agent with a broad spectrum that worth further study. Hopefully, the structure QUB-1957 could be researched for novel antimicrobial agent development."]},{"key":"dc:title","label":"Title","values":["QUB-1957: a bioactive peptide from the frog skin secretion of Hylarana latouchii"]}]}],"canonical_facts":{"dc:contributor.advisor":["Chen, Tianbao","Zhou, Mei","Wang, Lei","Shaw, Christopher"],"dc:creator":["Yang, Ruoning"],"dc:date":["2018-7"],"dc:date.issued":["2018-7"],"dc:description.abstract":["The skin secretions of amphibians are significant for amphibians’ daily protection, especially the antimicrobial peptides. In this study, a cDNA library from lyophilised skin secretion of Hylarana latouchii was constructed, and molecular cloning of biosynthetic precursor-encoding cDNAs of QUB-1957 was performed. Subsequently, QUB-1957 was synthesised by Solid Phase Peptide Synthesizer. In the antimicrobial assays, the MIC of QUB-1957 against Gram-positive bacteria Staphylococcus aureus, Gram-negative bacteria Escherichia coli and yeast Candida albicans are 8 μM, 16 μM and 16 μM, respectively. Unfortunately, QUB-1957 also exhibits a certain degree of haemolytic rate, the HC50 is 47.82 μM. Besides, QUB-1957 exhibits no significant effect against PC3 (human prostate cancer cell line), NCI-H157 (human non-small cell lung cancer cell line), MCF-7 (human breast cancer cell line) and U251MG (human neuronal glioblastoma cell line). These characteristics of QUB-1957 indicate that QUB-1957 is a promising antimicrobial agent with a broad spectrum that worth further study. 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