LINEAR AND NONLINEAR FLUORESCENCE LIFETIME IMAGING FOR BIOSENSING APPLICATIONS

Fluorescence techniques, based on both linear and non-linear excitation, have been widely used in biosensing application. Among fluorescence techniques, Fluorescence Lifetime Imaging (FLIM) has gained increased importance because of their robust and versatile feature. FLIM is a technique to visualize the spatial distribution of picosecond to nanosecond excited state lifetimes within microscopic images. In this work, FLIM technique combined with Time Correlated Single Photon Counting (TCPSC) Technology was used to develop several novel biosensing approaches. Using linear or nonlinear excitation source, TCSPC-FLIM enabled real time monitoring of cellular metabolite profiles, as well as longitudinal observation of post-translational enzyme activity such as kinase phosphorylation and acetyltransferase acetylation. This technique will not only provide high spatiotemporal resolution of sensing output but also for the first time, realize real-time biosensing in live intact single cell within 2 D culture, 3D culture and in-vivo animal model, zebrafish.

Name thesis:degree_name

Doctor of Philosophy (PhD)

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Agricultural and Biological Engineering

Year

2016

Author dc:creator

• Damayanti, Nur Pradani

Contributors dc:contributor

• JOSEPH MK IRUDAYARAJ
• PAUL J ROBINSON
• MENG DENG
• JENNA RICKUS

• ACETYLATION
• FLIM
• KINASE
• METABOLITE
• PHOSPHORYLATION
• SENSOR

Repository record dc:identifier

https://docs.lib.purdue.edu/open_access_dissertations/1243

OAI identifier oai:identifier

oai:docs.lib.purdue.edu:open_access_dissertations-2459