{"id":{"repo_id":"purdue-thes","oai_identifier":"oai:docs.lib.purdue.edu:open_access_dissertations-2459"},"canonical_url":"https://search.dev.ndltd.org/etd/purdue-thes/oai:docs.lib.purdue.edu:open_access_dissertations-2459","repository":{"repo_id":"purdue-thes","name":"Purdue University","base_url":"https://docs.lib.purdue.edu/do/oai/"},"display":{"title":"LINEAR AND NONLINEAR FLUORESCENCE LIFETIME IMAGING FOR BIOSENSING APPLICATIONS","abstract":"Fluorescence techniques, based on both linear and non-linear excitation, have been widely used in biosensing application. Among fluorescence techniques, Fluorescence Lifetime Imaging (FLIM) has gained increased importance because of their robust and versatile feature. FLIM is a technique to visualize the spatial distribution of picosecond to nanosecond excited state lifetimes within microscopic images. In this work, FLIM technique combined with Time Correlated Single Photon Counting (TCPSC) Technology was used to develop several novel biosensing approaches. Using linear or nonlinear excitation source, TCSPC-FLIM enabled real time monitoring of cellular metabolite profiles, as well as longitudinal observation of post-translational enzyme activity such as kinase phosphorylation and acetyltransferase acetylation. This technique will not only provide high spatiotemporal resolution of sensing output but also for the first time, realize real-time biosensing in live intact single cell within 2 D culture, 3D culture and in-vivo animal model, zebrafish.","abstract_html":"Fluorescence techniques, based on both linear and non-linear excitation, have been widely used in biosensing application. Among fluorescence techniques, Fluorescence Lifetime Imaging (FLIM) has gained increased importance because of their robust and versatile feature. FLIM is a technique to visualize the spatial distribution of picosecond to nanosecond excited state lifetimes within microscopic images. In this work, FLIM technique combined with Time Correlated Single Photon Counting (TCPSC) Technology was used to develop several novel biosensing approaches. Using linear or nonlinear excitation source, TCSPC-FLIM enabled real time monitoring of cellular metabolite profiles, as well as longitudinal observation of post-translational enzyme activity such as kinase phosphorylation and acetyltransferase acetylation. This technique will not only provide high spatiotemporal resolution of sensing output but also for the first time, realize real-time biosensing in live intact single cell within 2 D culture, 3D culture and in-vivo animal model, zebrafish.","abstract_has_math":false,"creators":["Damayanti, Nur Pradani"],"institution":null,"degree_name":"Doctor of Philosophy (PhD)","degree_level":"Dissertation","degree_discipline":"Agricultural and Biological Engineering","degree_department":null,"school":null,"contributors":["JOSEPH MK IRUDAYARAJ","PAUL J ROBINSON","MENG DENG","JENNA RICKUS"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2016,"date_issued":"2016-01-01T08:00:00Z","date_published":"2016-01-01T08:00:00Z","updated_at":"2026-07-24T03:54:31Z","subjects":["ACETYLATION","FLIM","KINASE","METABOLITE","PHOSPHORYLATION","SENSOR"],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://docs.lib.purdue.edu/open_access_dissertations/1243","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["JOSEPH MK IRUDAYARAJ","PAUL J ROBINSON","MENG DENG","JENNA RICKUS"]},{"key":"dc:creator","label":"Author","values":["Damayanti, Nur Pradani"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"thesis:degree_discipline","label":"Discipline","values":["Agricultural and Biological Engineering"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Doctor of Philosophy (PhD)"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["ACETYLATION","FLIM","KINASE","METABOLITE","PHOSPHORYLATION","SENSOR"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://docs.lib.purdue.edu/open_access_dissertations/1243"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Fluorescence techniques, based on both linear and non-linear excitation, have been widely used in biosensing application. Among fluorescence techniques, Fluorescence Lifetime Imaging (FLIM) has gained increased importance because of their robust and versatile feature. FLIM is a technique to visualize the spatial distribution of picosecond to nanosecond excited state lifetimes within microscopic images. In this work, FLIM technique combined with Time Correlated Single Photon Counting (TCPSC) Technology was used to develop several novel biosensing approaches. Using linear or nonlinear excitation source, TCSPC-FLIM enabled real time monitoring of cellular metabolite profiles, as well as longitudinal observation of post-translational enzyme activity such as kinase phosphorylation and acetyltransferase acetylation. This technique will not only provide high spatiotemporal resolution of sensing output but also for the first time, realize real-time biosensing in live intact single cell within 2 D culture, 3D culture and in-vivo animal model, zebrafish."]},{"key":"dc:title","label":"Title","values":["LINEAR AND NONLINEAR FLUORESCENCE LIFETIME IMAGING FOR BIOSENSING APPLICATIONS"]}]}],"canonical_facts":{"dc:contributor":["JOSEPH MK IRUDAYARAJ","PAUL J ROBINSON","MENG DENG","JENNA RICKUS"],"dc:creator":["Damayanti, Nur Pradani"],"dc:description.abstract":["Fluorescence techniques, based on both linear and non-linear excitation, have been widely used in biosensing application. Among fluorescence techniques, Fluorescence Lifetime Imaging (FLIM) has gained increased importance because of their robust and versatile feature. FLIM is a technique to visualize the spatial distribution of picosecond to nanosecond excited state lifetimes within microscopic images. In this work, FLIM technique combined with Time Correlated Single Photon Counting (TCPSC) Technology was used to develop several novel biosensing approaches. Using linear or nonlinear excitation source, TCSPC-FLIM enabled real time monitoring of cellular metabolite profiles, as well as longitudinal observation of post-translational enzyme activity such as kinase phosphorylation and acetyltransferase acetylation. This technique will not only provide high spatiotemporal resolution of sensing output but also for the first time, realize real-time biosensing in live intact single cell within 2 D culture, 3D culture and in-vivo animal model, zebrafish."],"dc:identifier":["https://docs.lib.purdue.edu/open_access_dissertations/1243"],"dc:subject":["ACETYLATION","FLIM","KINASE","METABOLITE","PHOSPHORYLATION","SENSOR"],"dc:title":["LINEAR AND NONLINEAR FLUORESCENCE LIFETIME IMAGING FOR BIOSENSING APPLICATIONS"],"thesis:degree_discipline":["Agricultural and Biological Engineering"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Doctor of Philosophy (PhD)"]},"updated_at":"2026-07-24T03:54:31Z"}