Isolation and characterisation of toxins from Fibrocapsa japonica (Raphidophyceae)

Harmful Algal Blooms (HAB) is a natural disaster which has attracted global attention in recent decades since it threatens greatly public health, causes economic damage to fisheries and tourism. Fibrocapsa japonica is one of HAB causative organisms which caused significant loss to coastal fisheries in Japan. From the 1990's it was also reported frequently in European coastal waters. The toxicity mechanism behind such fish killing events is so far unclear. Although some views of fibrocapsins (toxins produced by this alga) were ever reported e.g. brevetoxins, but not acceptable for lack of conclusiveness. In this work, the toxicity of Fibrocapsa japonica algal cells was first established by Artemia salina biotest. Fibrocapsins was screened step by step through Artemia salina biotest, bioluminescence inhibition biotest and erythrocyte lysis assay methods, isolated then in HPLC. The chemical natures of fibrocapsins 1, 2 and 3 were determined finally as 6,9,12,15-octadecatetraenoic acid, all-cis-5,8,11,14,17-eicosapentaenoic acid and all-cis-5,8,11,14-eicosatetraenoic acid by HPLC-MS, IR, GC-(HR)MS, NMR experiments and biotest.

Level thesis:degree_level

thesis.doctoral

Grantor dc:publisher

Universität Oldenburg

Year

2003

Author dc:creator

• Fu, Meng

• [Keine Schlagwörter von Autor/in vergeben.]

Repository record source_url

http://oops.uni-oldenburg.de/240

OAI identifier oai:identifier

oai:oops.uni-oldenburg.de:240