HYPOXIA-REGULATED EXPRESSION OF GLUT-1 IN GBM CELL LINES

<p>Unlike normal cells, cancer cells can grow in low oxygen (hypoxic) environments. Changes in relative oxygen concentration can alter gene expression in tumors to allow for their selective growth. The result of such changes allows the tumor to adapt its cellular metabolism and promote tumor progression. Most notably, hypoxic conditions induce expression of the transcription factor hypoxia inducible factor 1 (HIF-1). HIF-1 is thought to directly affect glucose transporter 1 (GLUT1) expression levels in hypoxic conditions. This study sought to determine the relationship between HIF-1 and GLUT1 expression levels within normoxic and hypoxic environments utilizing an <em>in-vitro</em> GBM model. The data accumulated in this study determined that hypoxia correlates with relative expression levels of hypoxia inducible factors (HIFs) as well as GLUT1. HIF-1b and GLUT1 appear to have different expression levels in fibroblast cells than in GBM cells, solidifying that HIF-1b and GLUT1 are suitable targets for future GBM treatments.</p>

Name thesis:degree_name

Master of Science

Level thesis:degree_level

Thesis

Discipline thesis:degree_discipline

Biology

Year dc:date.available

2018

Author dc:creator

• Kane, Marissa

Contributors dc:contributor

• Robert Belton

• Glucose Transporter 1
• GLUT1
• Warburg Effect
• Hypoxia Inducible Factors
• aerobic glycolysis
• Glioblastoma multiforme
• cancer cell metabolism
• hypoxia
• hypoxic tumor microenvironment
• HIFs
• Cancer Biology

Repository record dc:identifier

https://commons.nmu.edu/theses/554

OAI identifier oai:identifier

oai:commons.nmu.edu:theses-1573