Unlike normal cells, cancer cells can grow in low oxygen (hypoxic) environments. Changes in relative oxygen concentration can alter gene expression in tumors to allow for their selective growth. The result of such changes allows the tumor to adapt its cellular metabolism and promote tumor progression. Most notably, hypoxic conditions induce expression of the transcription factor hypoxia inducible factor 1 (HIF-1). HIF-1 is thought to directly affect glucose transporter 1 (GLUT1) expression levels in hypoxic conditions. This study sought to determine the relationship between HIF-1 and GLUT1 expression levels within normoxic and hypoxic environments utilizing an in-vitro GBM model. The data accumulated in this study determined that hypoxia correlates with relative expression levels of hypoxia inducible factors (HIFs) as well as GLUT1. HIF-1b and GLUT1 appear to have different expression levels in fibroblast cells than in GBM cells, solidifying that HIF-1b and GLUT1 are suitable targets for future GBM treatments.
","abstract_html":"<p>Unlike normal cells, cancer cells can grow in low oxygen (hypoxic) environments. Changes in relative oxygen concentration can alter gene expression in tumors to allow for their selective growth. The result of such changes allows the tumor to adapt its cellular metabolism and promote tumor progression. Most notably, hypoxic conditions induce expression of the transcription factor hypoxia inducible factor 1 (HIF-1). HIF-1 is thought to directly affect glucose transporter 1 (GLUT1) expression levels in hypoxic conditions. This study sought to determine the relationship between HIF-1 and GLUT1 expression levels within normoxic and hypoxic environments utilizing an <em>in-vitro</em> GBM model. The data accumulated in this study determined that hypoxia correlates with relative expression levels of hypoxia inducible factors (HIFs) as well as GLUT1. HIF-1b and GLUT1 appear to have different expression levels in fibroblast cells than in GBM cells, solidifying that HIF-1b and GLUT1 are suitable targets for future GBM treatments.</p>","abstract_has_math":false,"creators":["Kane, Marissa"],"institution":null,"degree_name":"Master of Science","degree_level":"Thesis","degree_discipline":"Biology","degree_department":null,"school":null,"contributors":["Robert Belton"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2018,"date_issued":"2018-08-01T07:00:00Z","date_published":"2018-08-01T07:00:00Z","updated_at":"2026-07-24T03:24:12Z","subjects":["Glucose Transporter 1","GLUT1","Warburg Effect","Hypoxia Inducible Factors","aerobic glycolysis","Glioblastoma multiforme","cancer cell metabolism","hypoxia","hypoxic tumor microenvironment","HIFs","Cancer Biology"],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://commons.nmu.edu/theses/554","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Robert Belton"]},{"key":"dc:creator","label":"Author","values":["Kane, Marissa"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.available","label":"Dc Date Available","values":["2019-03-14T07:00:00Z"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Biology"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Master of Science"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Glucose Transporter 1","GLUT1","Warburg Effect","Hypoxia Inducible Factors","aerobic glycolysis","Glioblastoma multiforme","cancer cell metabolism","hypoxia","hypoxic tumor microenvironment","HIFs","Cancer Biology"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://commons.nmu.edu/theses/554"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Unlike normal cells, cancer cells can grow in low oxygen (hypoxic) environments. Changes in relative oxygen concentration can alter gene expression in tumors to allow for their selective growth. The result of such changes allows the tumor to adapt its cellular metabolism and promote tumor progression. Most notably, hypoxic conditions induce expression of the transcription factor hypoxia inducible factor 1 (HIF-1). HIF-1 is thought to directly affect glucose transporter 1 (GLUT1) expression levels in hypoxic conditions. This study sought to determine the relationship between HIF-1 and GLUT1 expression levels within normoxic and hypoxic environments utilizing an in-vitro GBM model. The data accumulated in this study determined that hypoxia correlates with relative expression levels of hypoxia inducible factors (HIFs) as well as GLUT1. HIF-1b and GLUT1 appear to have different expression levels in fibroblast cells than in GBM cells, solidifying that HIF-1b and GLUT1 are suitable targets for future GBM treatments.
"]},{"key":"dc:title","label":"Title","values":["HYPOXIA-REGULATED EXPRESSION OF GLUT-1 IN GBM CELL LINES"]}]}],"canonical_facts":{"dc:contributor":["Robert Belton"],"dc:creator":["Kane, Marissa"],"dc:date.available":["2019-03-14T07:00:00Z"],"dc:description.abstract":["Unlike normal cells, cancer cells can grow in low oxygen (hypoxic) environments. Changes in relative oxygen concentration can alter gene expression in tumors to allow for their selective growth. The result of such changes allows the tumor to adapt its cellular metabolism and promote tumor progression. Most notably, hypoxic conditions induce expression of the transcription factor hypoxia inducible factor 1 (HIF-1). HIF-1 is thought to directly affect glucose transporter 1 (GLUT1) expression levels in hypoxic conditions. This study sought to determine the relationship between HIF-1 and GLUT1 expression levels within normoxic and hypoxic environments utilizing an in-vitro GBM model. The data accumulated in this study determined that hypoxia correlates with relative expression levels of hypoxia inducible factors (HIFs) as well as GLUT1. HIF-1b and GLUT1 appear to have different expression levels in fibroblast cells than in GBM cells, solidifying that HIF-1b and GLUT1 are suitable targets for future GBM treatments.
"],"dc:identifier":["https://commons.nmu.edu/theses/554"],"dc:subject":["Glucose Transporter 1","GLUT1","Warburg Effect","Hypoxia Inducible Factors","aerobic glycolysis","Glioblastoma multiforme","cancer cell metabolism","hypoxia","hypoxic tumor microenvironment","HIFs","Cancer Biology"],"dc:title":["HYPOXIA-REGULATED EXPRESSION OF GLUT-1 IN GBM CELL LINES"],"thesis:degree_discipline":["Biology"],"thesis:degree_level":["Thesis"],"thesis:degree_name":["Master of Science"]},"updated_at":"2026-07-24T03:24:12Z"}