Triplex dna receptor-gold nanoparticle conjugates for detection of oxidized dna base

Oxidations of DNA nucleobases are believed to be one of the sources of aging and some age-dependent disease, such as cancer. Human body have various mechanisms to repair damaged DNA and base-excision-repair is one mechanism for removing those oxidized nucleobases. This mechanism processes in the cells by cutting the oxidized nucleobases from deoxyribose and letting them excrete though urine. Detection of those abnormal nucleobases in urine has been suggested as a convenient way of early cancer diagnosis. 5-OHU (5-hydroxyuracil), the oxidation product of cytosine, is one of those abnormal nucleobases and it potentially forms base pairs with all four DNA nucleobases, which may in the end cause mutation. In this project, a binding cavity for 5-OHU in triplex system was designed and triplex conjugated gold nanoparticles were synthesized. The melting temperature of the triplex DNA-GNP conjugates were 5-OHU concentration dependent, which demonstrated the potential of the system for colorimetric detection of urinary 5-OHU.

Name thesis:degree_name

Master of Science in Chemistry - (M.S.)

Discipline thesis:degree_discipline

Chemistry and Environmental Science

Year

2016

Author dc:creator

• Xing, Qingyu

Contributors dc:contributor

• Edgardo Tabion Farinas
• Haidong Huang
• Zafar Iqbal

• DNA nucleobase oxidation
• Base-excision-repair
• Oxidized dna base detection
• Chemistry

Repository record dc:identifier

https://digitalcommons.njit.edu/theses/265

OAI identifier oai:identifier

oai:digitalcommons.njit.edu:theses-1264