Pil1, an Eisosome Organizer, Plays an Important Role in the Recruitment of Synaptojanins and Amphiphysins to Facilitate Receptor-Mediated Endocytosis in Yeast

The eisosome protein Pil1 is known to be implicated in the endocytosis of Ste3, but the precise biological function of it during endocytosis is poorly understood. Here, I present data to reveal Pil1's role in receptor-mediated endocytosis. Using live cell imaging, I show that endocytic patches carrying Abp1 and Las17 persisted much longer in PIL1-deficient cells (pil1Δ). The loss of Pil1 also greatly affected both the scission efficiency and the frequency of formation of endocytic sites carrying Rvs161- and Rvs167-GFP. Furthermore, the mistargeting of the synaptojanins, Sjl1 and Sjl2, to the cytoplasm in pil1Δ cells suggests that Pil1 is required for the proper recruitment of the synaptojanins to endocytic sites. A severe motility defect of Abp1-GFP during its internalization in a codeletant of PIL1 and SJL2 indicates a functional interplay between them in endocytosis. Together, these results establish that Pil1 is involved in the recruitment of endocytic proteins to optimize endocytosis.

Name thesis:degree_name

Master of Science in Biology

Level thesis:degree_level

Masters

Discipline thesis:degree_discipline

Biology

Year

2011

Author dc:creator

• Murphy, Erin Ruth

Contributors dc:contributor

• Kyoungtae Kim

• eisosome
• Pil1
• synaptojanin
• amphiphysin
• receptor-mediated endocytosis
• yeast
• Biology

Statement dc:rights

• © Erin Ruth Murphy

Repository record dc:identifier

https://bearworks.missouristate.edu/theses/1287

OAI identifier oai:identifier

oai:bearworks.missouristate.edu:theses-2288