{"id":{"repo_id":"mo-state","oai_identifier":"oai:bearworks.missouristate.edu:theses-2288"},"canonical_url":"https://search.dev.ndltd.org/etd/mo-state/oai:bearworks.missouristate.edu:theses-2288","repository":{"repo_id":"mo-state","name":"Missouri State University","base_url":"https://bearworks.missouristate.edu/do/oai/"},"display":{"title":"Pil1, an Eisosome Organizer, Plays an Important Role in the Recruitment of Synaptojanins and Amphiphysins to Facilitate Receptor-Mediated Endocytosis in Yeast","abstract":"The eisosome protein Pil1 is known to be implicated in the endocytosis of Ste3, but the precise biological function of it during endocytosis is poorly understood. Here, I present data to reveal Pil1's role in receptor-mediated endocytosis. Using live cell imaging, I show that endocytic patches carrying Abp1 and Las17 persisted much longer in PIL1-deficient cells (pil1Δ). The loss of Pil1 also greatly affected both the scission efficiency and the frequency of formation of endocytic sites carrying Rvs161- and Rvs167-GFP. Furthermore, the mistargeting of the synaptojanins, Sjl1 and Sjl2, to the cytoplasm in pil1Δ cells suggests that Pil1 is required for the proper recruitment of the synaptojanins to endocytic sites. A severe motility defect of Abp1-GFP during its internalization in a codeletant of PIL1 and SJL2 indicates a functional interplay between them in endocytosis. Together, these results establish that Pil1 is involved in the recruitment of endocytic proteins to optimize endocytosis.","abstract_html":"The eisosome protein Pil1 is known to be implicated in the endocytosis of Ste3, but the precise biological function of it during endocytosis is poorly understood. Here, I present data to reveal Pil1's role in receptor-mediated endocytosis. Using live cell imaging, I show that endocytic patches carrying Abp1 and Las17 persisted much longer in PIL1-deficient cells (pil1Δ). The loss of Pil1 also greatly affected both the scission efficiency and the frequency of formation of endocytic sites carrying Rvs161- and Rvs167-GFP. Furthermore, the mistargeting of the synaptojanins, Sjl1 and Sjl2, to the cytoplasm in pil1Δ cells suggests that Pil1 is required for the proper recruitment of the synaptojanins to endocytic sites. A severe motility defect of Abp1-GFP during its internalization in a codeletant of PIL1 and SJL2 indicates a functional interplay between them in endocytosis. Together, these results establish that Pil1 is involved in the recruitment of endocytic proteins to optimize endocytosis.","abstract_has_math":false,"creators":["Murphy, Erin Ruth"],"institution":null,"degree_name":"Master of Science in Biology","degree_level":"Masters","degree_discipline":"Biology","degree_department":null,"school":null,"contributors":["Kyoungtae Kim"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2011,"date_issued":"2011-05-01T07:00:00Z","date_published":"2011-05-01T07:00:00Z","updated_at":"2026-07-24T03:16:17Z","subjects":["eisosome","Pil1","synaptojanin","amphiphysin","receptor-mediated endocytosis","yeast","Biology"],"languages":[],"rights":["© Erin Ruth Murphy"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://bearworks.missouristate.edu/theses/1287","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Kyoungtae Kim"]},{"key":"dc:creator","label":"Author","values":["Murphy, Erin Ruth"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"thesis:degree_discipline","label":"Discipline","values":["Biology"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Masters"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Master of Science in Biology"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["eisosome","Pil1","synaptojanin","amphiphysin","receptor-mediated endocytosis","yeast","Biology"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:rights","label":"Dc Rights","values":["© Erin Ruth Murphy"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://bearworks.missouristate.edu/theses/1287"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["The eisosome protein Pil1 is known to be implicated in the endocytosis of Ste3, but the precise biological function of it during endocytosis is poorly understood. Here, I present data to reveal Pil1's role in receptor-mediated endocytosis. Using live cell imaging, I show that endocytic patches carrying Abp1 and Las17 persisted much longer in PIL1-deficient cells (pil1Δ). The loss of Pil1 also greatly affected both the scission efficiency and the frequency of formation of endocytic sites carrying Rvs161- and Rvs167-GFP. Furthermore, the mistargeting of the synaptojanins, Sjl1 and Sjl2, to the cytoplasm in pil1Δ cells suggests that Pil1 is required for the proper recruitment of the synaptojanins to endocytic sites. A severe motility defect of Abp1-GFP during its internalization in a codeletant of PIL1 and SJL2 indicates a functional interplay between them in endocytosis. Together, these results establish that Pil1 is involved in the recruitment of endocytic proteins to optimize endocytosis."]},{"key":"dc:title","label":"Title","values":["Pil1, an Eisosome Organizer, Plays an Important Role in the Recruitment of Synaptojanins and Amphiphysins to Facilitate Receptor-Mediated Endocytosis in Yeast"]}]}],"canonical_facts":{"dc:contributor":["Kyoungtae Kim"],"dc:creator":["Murphy, Erin Ruth"],"dc:description.abstract":["The eisosome protein Pil1 is known to be implicated in the endocytosis of Ste3, but the precise biological function of it during endocytosis is poorly understood. Here, I present data to reveal Pil1's role in receptor-mediated endocytosis. Using live cell imaging, I show that endocytic patches carrying Abp1 and Las17 persisted much longer in PIL1-deficient cells (pil1Δ). The loss of Pil1 also greatly affected both the scission efficiency and the frequency of formation of endocytic sites carrying Rvs161- and Rvs167-GFP. Furthermore, the mistargeting of the synaptojanins, Sjl1 and Sjl2, to the cytoplasm in pil1Δ cells suggests that Pil1 is required for the proper recruitment of the synaptojanins to endocytic sites. A severe motility defect of Abp1-GFP during its internalization in a codeletant of PIL1 and SJL2 indicates a functional interplay between them in endocytosis. Together, these results establish that Pil1 is involved in the recruitment of endocytic proteins to optimize endocytosis."],"dc:identifier":["https://bearworks.missouristate.edu/theses/1287"],"dc:rights":["© Erin Ruth Murphy"],"dc:subject":["eisosome","Pil1","synaptojanin","amphiphysin","receptor-mediated endocytosis","yeast","Biology"],"dc:title":["Pil1, an Eisosome Organizer, Plays an Important Role in the Recruitment of Synaptojanins and Amphiphysins to Facilitate Receptor-Mediated Endocytosis in Yeast"],"thesis:degree_discipline":["Biology"],"thesis:degree_level":["Masters"],"thesis:degree_name":["Master of Science in Biology"]},"updated_at":"2026-07-24T03:16:17Z"}