Extending imaging depth of multiphoton microscopy

Two-photon excitation fluorescence microscopy has capability of deep tissue imaging with biological samples. However, because of the inhomogeneity of the refractive index in biological samples, the wavefront of the excitation light is often distorted. Due to the distortion of the wavefront, the point spread function at the focal point becomes broadened resulting in degraded resolution and lower signal. With an adaptive optics system, which consists of a wavefront camera and deformable mirror, the wavefront distortion can be measured and corrected. By correcting the distorted wavefront with adaptive optics, resolution and signal level can be preserved at greater imaging depth.

Department dc:contributor.department

Massachusetts Institute of Technology. Dept. of Mechanical Engineering.

Grantor dc:publisher

Massachusetts Institute of Technology

Year dc:date.issued

2007

Author dc:creator

• Cha, Jae Won

Advisor dc:contributor.advisor

• Peter T.C. So.

• Mechanical Engineering.

Statement dc:rights

• M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission.

Licence dc:rights.uri

• http://dspace.mit.edu/handle/1721.1/7582

Language dc:language.iso

eng

Handle dc:identifier.uri

http://hdl.handle.net/1721.1/42299

OAI identifier oai:identifier

oai:dspace.mit.edu:1721.1/42299