{"id":{"repo_id":"mit","oai_identifier":"oai:dspace.mit.edu:1721.1/42299"},"canonical_url":"https://search.dev.ndltd.org/etd/mit/oai:dspace.mit.edu:1721.1/42299","repository":{"repo_id":"mit","name":"MIT","base_url":"https://dspace.mit.edu/oai/request"},"display":{"title":"Extending imaging depth of multiphoton microscopy","abstract":"Two-photon excitation fluorescence microscopy has capability of deep tissue imaging with biological samples. However, because of the inhomogeneity of the refractive index in biological samples, the wavefront of the excitation light is often distorted. Due to the distortion of the wavefront, the point spread function at the focal point becomes broadened resulting in degraded resolution and lower signal. With an adaptive optics system, which consists of a wavefront camera and deformable mirror, the wavefront distortion can be measured and corrected. By correcting the distorted wavefront with adaptive optics, resolution and signal level can be preserved at greater imaging depth.","abstract_html":"Two-photon excitation fluorescence microscopy has capability of deep tissue imaging with biological samples. However, because of the inhomogeneity of the refractive index in biological samples, the wavefront of the excitation light is often distorted. Due to the distortion of the wavefront, the point spread function at the focal point becomes broadened resulting in degraded resolution and lower signal. With an adaptive optics system, which consists of a wavefront camera and deformable mirror, the wavefront distortion can be measured and corrected. By correcting the distorted wavefront with adaptive optics, resolution and signal level can be preserved at greater imaging depth.","abstract_has_math":false,"creators":["Cha, Jae Won"],"institution":"Massachusetts Institute of Technology","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":"Massachusetts Institute of Technology. Dept. of Mechanical Engineering.","school":null,"contributors":[],"advisors":["Peter T.C. So."],"committee_chairs":[],"committee_members":[],"year":2007,"date_issued":"2007","date_published":"2007","updated_at":"2026-07-22T22:20:56Z","subjects":["Mechanical Engineering."],"languages":["eng"],"rights":["M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission."],"rights_urls":["http://dspace.mit.edu/handle/1721.1/7582"],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/1721.1/42299","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Peter T.C. So."]},{"key":"dc:contributor.department","label":"Department","values":["Massachusetts Institute of Technology. Dept. of Mechanical Engineering."]},{"key":"dc:contributor.other","label":"Dc Contributor Other","values":["Massachusetts Institute of Technology. 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However, because of the inhomogeneity of the refractive index in biological samples, the wavefront of the excitation light is often distorted. Due to the distortion of the wavefront, the point spread function at the focal point becomes broadened resulting in degraded resolution and lower signal. With an adaptive optics system, which consists of a wavefront camera and deformable mirror, the wavefront distortion can be measured and corrected. By correcting the distorted wavefront with adaptive optics, resolution and signal level can be preserved at greater imaging depth."]},{"key":"dc:description.degree","label":"Dc Description Degree","values":["S.M."]},{"key":"dc:title","label":"Title","values":["Extending imaging depth of multiphoton microscopy"]}]}],"canonical_facts":{"dc:contributor.advisor":["Peter T.C. So."],"dc:contributor.department":["Massachusetts Institute of Technology. Dept. of Mechanical Engineering."],"dc:contributor.other":["Massachusetts Institute of Technology. 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