Comparison of Two Techniques for Enumeration of Human B and T Lymphocytes

<p>The assay of Bursa-derived and Thymus-derived lymphocytes (B and T cells) in man has become increasingly useful during the past few years in normal and in clinical conditions. There has been quite a range reported in the literature in the percent of B and T cells in normal subjects by different investigators.</p> <p>The purpose of this study was to see if monocyte contamination was one cause of non-specific rosette formation. Twenty-one subjects were used to compare the technique involving trypsin and neuraminidase treatment of erythrocytes and the technique of glass wool removal of monocytes. A peroxidase stain was used to confirm monocytes on ten of the twenty-one subjects and a fluorescent antibody stain was done on four subjects to confirm the EAC rosettes as B cells.</p> <p>There was a definite decrease in the number of monocytes after glass wool column separation. This led to a slight increase in the number of T cells and a slight decrease of B cells.</p> <p>A method is described which will help eliminate more of the monocytes and give a more accurate value of B and T cells.</p>

Name thesis:degree_name

Master of Science (MS)

Level thesis:degree_level

Thesis

Discipline thesis:degree_discipline

Microbiology

Year

1976

Author dc:creator

• Forsythe, William F., Jr.

Contributors dc:contributor

• Benjamin H. S. Lau
• Charles E. Winter
• Earl W. Lathrop

• Microbiology
• Lymphocytes; B-Lymphocytes; T-Lymphocytes.

Statement dc:rights

• This title appears here courtesy of the author, who has granted Loma Linda University a limited, non-exclusive right to make this publication available to the public. The author retains all other copyrights.

Language dc:language

English

Repository record dc:identifier

https://scholarsrepository.llu.edu/etd/1381

OAI identifier oai:identifier

oai:scholarsrepository.llu.edu:etd-2147