The assay of Bursa-derived and Thymus-derived lymphocytes (B and T cells) in man has become increasingly useful during the past few years in normal and in clinical conditions. There has been quite a range reported in the literature in the percent of B and T cells in normal subjects by different investigators.
The purpose of this study was to see if monocyte contamination was one cause of non-specific rosette formation. Twenty-one subjects were used to compare the technique involving trypsin and neuraminidase treatment of erythrocytes and the technique of glass wool removal of monocytes. A peroxidase stain was used to confirm monocytes on ten of the twenty-one subjects and a fluorescent antibody stain was done on four subjects to confirm the EAC rosettes as B cells.
There was a definite decrease in the number of monocytes after glass wool column separation. This led to a slight increase in the number of T cells and a slight decrease of B cells.
A method is described which will help eliminate more of the monocytes and give a more accurate value of B and T cells.
","abstract_html":"<p>The assay of Bursa-derived and Thymus-derived lymphocytes (B and T cells) in man has become increasingly useful during the past few years in normal and in clinical conditions. There has been quite a range reported in the literature in the percent of B and T cells in normal subjects by different investigators.</p> <p>The purpose of this study was to see if monocyte contamination was one cause of non-specific rosette formation. Twenty-one subjects were used to compare the technique involving trypsin and neuraminidase treatment of erythrocytes and the technique of glass wool removal of monocytes. A peroxidase stain was used to confirm monocytes on ten of the twenty-one subjects and a fluorescent antibody stain was done on four subjects to confirm the EAC rosettes as B cells.</p> <p>There was a definite decrease in the number of monocytes after glass wool column separation. This led to a slight increase in the number of T cells and a slight decrease of B cells.</p> <p>A method is described which will help eliminate more of the monocytes and give a more accurate value of B and T cells.</p>","abstract_has_math":false,"creators":["Forsythe, William F., Jr."],"institution":null,"degree_name":"Master of Science (MS)","degree_level":"Thesis","degree_discipline":"Microbiology","degree_department":null,"school":null,"contributors":["Benjamin H. S. Lau","Charles E. Winter","Earl W. Lathrop"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":1976,"date_issued":"1976-06-01T07:00:00Z","date_published":"1976-06-01T07:00:00Z","updated_at":"2026-07-24T02:53:44Z","subjects":["Microbiology","Lymphocytes; B-Lymphocytes; T-Lymphocytes."],"languages":["English"],"rights":["This title appears here courtesy of the author, who has granted Loma Linda University a limited, non-exclusive right to make this publication available to the public. The author retains all other copyrights."],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://scholarsrepository.llu.edu/etd/1381","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Benjamin H. S. Lau","Charles E. Winter","Earl W. Lathrop"]},{"key":"dc:creator","label":"Author","values":["Forsythe, William F., Jr."]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"thesis:degree_discipline","label":"Discipline","values":["Microbiology"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Master of Science (MS)"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Microbiology","Lymphocytes; B-Lymphocytes; T-Lymphocytes."]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["English"]},{"key":"dc:rights","label":"Dc Rights","values":["This title appears here courtesy of the author, who has granted Loma Linda University a limited, non-exclusive right to make this publication available to the public. The author retains all other copyrights."]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://scholarsrepository.llu.edu/etd/1381"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["The assay of Bursa-derived and Thymus-derived lymphocytes (B and T cells) in man has become increasingly useful during the past few years in normal and in clinical conditions. There has been quite a range reported in the literature in the percent of B and T cells in normal subjects by different investigators.
The purpose of this study was to see if monocyte contamination was one cause of non-specific rosette formation. Twenty-one subjects were used to compare the technique involving trypsin and neuraminidase treatment of erythrocytes and the technique of glass wool removal of monocytes. A peroxidase stain was used to confirm monocytes on ten of the twenty-one subjects and a fluorescent antibody stain was done on four subjects to confirm the EAC rosettes as B cells.
There was a definite decrease in the number of monocytes after glass wool column separation. This led to a slight increase in the number of T cells and a slight decrease of B cells.
A method is described which will help eliminate more of the monocytes and give a more accurate value of B and T cells.
"]},{"key":"dc:title","label":"Title","values":["Comparison of Two Techniques for Enumeration of Human B and T Lymphocytes"]}]}],"canonical_facts":{"dc:contributor":["Benjamin H. S. Lau","Charles E. Winter","Earl W. Lathrop"],"dc:creator":["Forsythe, William F., Jr."],"dc:description.abstract":["The assay of Bursa-derived and Thymus-derived lymphocytes (B and T cells) in man has become increasingly useful during the past few years in normal and in clinical conditions. There has been quite a range reported in the literature in the percent of B and T cells in normal subjects by different investigators.
The purpose of this study was to see if monocyte contamination was one cause of non-specific rosette formation. Twenty-one subjects were used to compare the technique involving trypsin and neuraminidase treatment of erythrocytes and the technique of glass wool removal of monocytes. A peroxidase stain was used to confirm monocytes on ten of the twenty-one subjects and a fluorescent antibody stain was done on four subjects to confirm the EAC rosettes as B cells.
There was a definite decrease in the number of monocytes after glass wool column separation. This led to a slight increase in the number of T cells and a slight decrease of B cells.
A method is described which will help eliminate more of the monocytes and give a more accurate value of B and T cells.
"],"dc:identifier":["https://scholarsrepository.llu.edu/etd/1381"],"dc:language":["English"],"dc:rights":["This title appears here courtesy of the author, who has granted Loma Linda University a limited, non-exclusive right to make this publication available to the public. The author retains all other copyrights."],"dc:subject":["Microbiology","Lymphocytes; B-Lymphocytes; T-Lymphocytes."],"dc:title":["Comparison of Two Techniques for Enumeration of Human B and T Lymphocytes"],"thesis:degree_discipline":["Microbiology"],"thesis:degree_level":["Thesis"],"thesis:degree_name":["Master of Science (MS)"]},"updated_at":"2026-07-24T02:53:44Z"}