Analysis of the Immunomodulatory Properties of Mycobacterium Cell Wall Fraction on the Cytokine Production of Peripheral Blood Mononuclear Cells of Healthy Dogs

Mycobacterium cell wall fraction (MCWF) is a Toll-like receptor agonist that is derived from non-pathogenic Mycobacterium phlei. The objective of this research was to evaluate host responses induced by MCWF in canine peripheral blood mononuclear cells (PBMCs), using enzyme-linked immunosorbent assays (ELISA) and quantitative reverse-transcription polymerase chain reaction (qRT-PCR) for quantifying cytokine protein and mRNA transcripts, respectively. PBMCs were isolated from eight healthy dogs and cells were cultured with MCWF. A significant increase of interleukin (IL)-10 mRNA was detected at all time points for all concentrations of MCWF (p<0.05). mRNA relative abundance reflected this finding. A post-stimulation elevation of interferon (IFN)-γ mRNA was noted at 24-hours with all concentrations of MCWF (p<0.01) and tumor necrosis factor (TNF)-α mRNA was increased for 0.5ng/dL MCWF only at 72-hours (p<0.05) compared to the negative control. Further research into the role of MCWF as a systemically administered immunomodulator or adjuvant to immunotherapy is warranted.

Grantor dc:publisher

University of Guelph

Author dc:creator

• Ward, Robert

Advisors dc:contributor.advisor

• Sharif, Shayan
• Wood, Geoffrey
• Karimi, Khalil
• Charlotte, Pye
• Anthony, Yu

• Mycobacterium Cell Wall Fraction
• Canine Peripheral Blood Mononuclear Cells
• Immunostimulant

Statement dc:rights

• Attribution-NonCommercial-ShareAlike 4.0 International

Licence dc:rights.uri

• http://creativecommons.org/licenses/by-nc-sa/4.0/

Language dc:language.iso

en

Handle dc:identifier.uri

https://hdl.handle.net/10214/28131

OAI record oai:identifier

https://atrium.lib.uoguelph.ca/server/oai/request?verb=GetRecord&metadataPrefix=dim&identifier=oai%3Aatrium.lib.uoguelph.ca%3A10214%2F28131