Molecular genetics of 2, 4-dichlorophenoxyacetate (2, 4-D) degradation in pseudomonas cepacia strain 2a

Pseudomonas cepacia strain 2a, which utilises2,4-dichlorophenoxyacetate (2,4-D) as a sole source of carbon and energy, has been shown to contain an 17kbp plasmid, p2,4-D, which has been mapped using BamHI and Asp718. Several mutants of strain 2a unable to grow on 2,4-D were generated by temperature curing. All bore plasmids of diminished size (57Kbp), created by deletion of 30Kbp from the parental plasmid. These were called p2,4-Del. The plasmid p2,4-Del was presumed to possess the origin of replication and related sequences but had lost some or all of the genes encoding enzymes for 2,4-D degradation.

Level dc:type.qualificationlevel

mphil

Grantor dc:publisher.institution

Thames Polytechnic

Year dc:date.issued

1992

Author dc:creator

• Aathithan, Sivasampu

• QH Natural history