{"id":{"repo_id":"greenwich","oai_identifier":"oai:gala.gre.ac.uk:10334"},"canonical_url":"https://search.dev.ndltd.org/etd/greenwich/oai:gala.gre.ac.uk:10334","repository":{"repo_id":"greenwich","name":"University of Greenwich","base_url":"https://gala.gre.ac.uk/cgi/oai2"},"display":{"title":"Molecular genetics of 2, 4-dichlorophenoxyacetate (2, 4-D) degradation in pseudomonas cepacia strain 2a","abstract":"Pseudomonas cepacia strain 2a, which utilises2,4-dichlorophenoxyacetate (2,4-D) as a sole source of carbon and energy, has been shown to contain an 17kbp plasmid, p2,4-D, which has been mapped using BamHI and Asp718. Several mutants of strain 2a unable to grow on 2,4-D were generated by temperature curing. All bore plasmids of diminished size (57Kbp), created by deletion of 30Kbp from the parental plasmid. These were called p2,4-Del. The plasmid p2,4-Del was presumed to possess the origin of replication and related sequences but had lost some or all of the genes encoding enzymes for 2,4-D degradation.","abstract_html":"Pseudomonas cepacia strain 2a, which utilises2,4-dichlorophenoxyacetate (2,4-D) as a sole source of carbon and energy, has been shown to contain an 17kbp plasmid, p2,4-D, which has been mapped using BamHI and Asp718. Several mutants of strain 2a unable to grow on 2,4-D were generated by temperature curing. All bore plasmids of diminished size (57Kbp), created by deletion of 30Kbp from the parental plasmid. These were called p2,4-Del. The plasmid p2,4-Del was presumed to possess the origin of replication and related sequences but had lost some or all of the genes encoding enzymes for 2,4-D degradation.","abstract_has_math":false,"creators":["Aathithan, Sivasampu"],"institution":"Thames Polytechnic","degree_name":null,"degree_level":"mphil","degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":1992,"date_issued":"1992","date_published":"1992","updated_at":"2026-07-24T02:25:47Z","subjects":["QH Natural history"],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":null,"outbound_label":null,"outbound_source":null},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["Aathithan, Sivasampu"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["1992"]},{"key":"dc:date.issued","label":"Date","values":["1992"]},{"key":"dc:publisher.department","label":"Dc Publisher Department","values":["School of Biological and Chemical Sciences"]},{"key":"dc:publisher.institution","label":"Dc Publisher Institution","values":["Thames Polytechnic"]},{"key":"dc:relation.isreferencedby","label":"Dc Relation Isreferencedby","values":["https://gala.gre.ac.uk/id/eprint/10334/"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]},{"key":"dc:type.qualificationlevel","label":"Dc Type Qualificationlevel","values":["mphil"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["QH Natural history"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Pseudomonas cepacia strain 2a, which utilises2,4-dichlorophenoxyacetate (2,4-D) as a sole source of carbon and energy, has been shown to contain an 17kbp plasmid, p2,4-D, which has been mapped using BamHI and Asp718. Several mutants of strain 2a unable to grow on 2,4-D were generated by temperature curing. All bore plasmids of diminished size (57Kbp), created by deletion of 30Kbp from the parental plasmid. These were called p2,4-Del. The plasmid p2,4-Del was presumed to possess the origin of replication and related sequences but had lost some or all of the genes encoding enzymes for 2,4-D degradation."]},{"key":"dc:title","label":"Title","values":["Molecular genetics of 2, 4-dichlorophenoxyacetate (2, 4-D) degradation in pseudomonas cepacia strain 2a"]}]}],"canonical_facts":{"dc:creator":["Aathithan, Sivasampu"],"dc:date":["1992"],"dc:date.issued":["1992"],"dc:description.abstract":["Pseudomonas cepacia strain 2a, which utilises2,4-dichlorophenoxyacetate (2,4-D) as a sole source of carbon and energy, has been shown to contain an 17kbp plasmid, p2,4-D, which has been mapped using BamHI and Asp718. Several mutants of strain 2a unable to grow on 2,4-D were generated by temperature curing. All bore plasmids of diminished size (57Kbp), created by deletion of 30Kbp from the parental plasmid. These were called p2,4-Del. The plasmid p2,4-Del was presumed to possess the origin of replication and related sequences but had lost some or all of the genes encoding enzymes for 2,4-D degradation."],"dc:publisher.department":["School of Biological and Chemical Sciences"],"dc:publisher.institution":["Thames Polytechnic"],"dc:relation.isreferencedby":["https://gala.gre.ac.uk/id/eprint/10334/"],"dc:subject":["QH Natural history"],"dc:title":["Molecular genetics of 2, 4-dichlorophenoxyacetate (2, 4-D) degradation in pseudomonas cepacia strain 2a"],"dc:type":["Thesis"],"dc:type.qualificationlevel":["mphil"]},"updated_at":"2026-07-24T02:25:47Z"}