Συγκριτική ανοσοϊστοχημική μελέτη υποδοχέων οιστρογόνων καρκίνου μαστού σε τομές κρυοστάτη και παραφίνης προς βιοχημικές και ανοσοενζυματικές μεθόδους

The monoclonal antibody ER-ICA (HSΡΓ-222) to human estrogen receptor protein amplified via the peroxidase-antiperoxidase (P.A.P.) method was used to detect the ER in cryostat, paraffin and acetone sections. In 72 of the 86 studied primary breast cancers, a comparative evaluation was done between the semiquantitative ER-ICA method and the quantitative enzyme immunoassay ER-EIA. In 24 of the total 86 cancers ER-ICA was compared with the biochemical dextran coated charcoal method (D.C.C.). The D.C.C. method was used for the simultaneous PG12 determination. An excellent agreement was found between the semiquantitative ER-ICA’s results in cryostat and paraffin, but not in acetone (AMEX) sections, concerning both, the percentage of positive or negative cells and the staining intensity. There was a linear correlation between the semiquantitative ER-ICA’s results in cryostat and paraffin sections (not in AMEX sections) and the quantitative ER-EIA’s results. Finally, a straight line correlation was observed between ER-EIA and D.C.C. method, regarding the ER but not the PGR determination.

Grantor dc:publisher

University of Ioannina

Year dc:date

1990

Authors dc:creator

• Stylianidou, Artemis
• Στυλιανίδου, Άρτεμις

• Ανοσοενζυματική μέθοδος
• Καρκίνωμα μαστού
• Μονοκλωνικά αντισώματα
• Προνάση
• Τομές ακετόνης
• Τομές παραφίνης
• Υποδοχείς οιστρογόνων
• Χρώση Ανοσοϋπεροξειδάσης
• Acetone sections
• Breast cancer
• Enzyme antibodies
• Estrogen receptors
• Immunoperoxidase staining
• Paraffin sections
• Pronase
• Ιατρική και Επιστήμες Υγείας
• Βασική Ιατρική
• Medical and Health Sciences
• Basic Medicine

Language dc:language

gre

Identifier

10.12681/eadd/1304

OAI identifier oai:identifier

oai:10442/1304