{"id":{"repo_id":"greece","oai_identifier":"oai:10442/1304"},"canonical_url":"https://search.dev.ndltd.org/etd/greece/oai:10442/1304","repository":{"repo_id":"greece","name":"Greek National Archive of PhD Theses","base_url":"https://phdtheses.ekt.gr/eadd_oai/request"},"display":{"title":"Συγκριτική ανοσοϊστοχημική μελέτη υποδοχέων οιστρογόνων καρκίνου μαστού σε τομές κρυοστάτη και παραφίνης προς βιοχημικές και ανοσοενζυματικές μεθόδους","abstract":"The monoclonal antibody ER-ICA (HSΡΓ-222) to human estrogen receptor protein amplified via the peroxidase-antiperoxidase (P.A.P.) method was used to detect the ER in cryostat, paraffin and acetone sections. In 72 of the 86 studied primary breast cancers, a comparative evaluation was done between the semiquantitative ER-ICA method and the quantitative enzyme immunoassay ER-EIA. In 24 of the total 86 cancers ER-ICA was compared with the biochemical dextran coated charcoal method (D.C.C.). The D.C.C. method was used for the simultaneous PG12 determination. An excellent agreement was found between the semiquantitative ER-ICA’s results in cryostat and paraffin, but not in acetone (AMEX) sections, concerning both, the percentage of positive or negative cells and the staining intensity. There was a linear correlation between the semiquantitative ER-ICA’s results in cryostat and paraffin sections (not in AMEX sections) and the quantitative ER-EIA’s results. Finally, a straight line correlation was observed between ER-EIA and D.C.C. method, regarding the ER but not the PGR determination.","abstract_html":"The monoclonal antibody ER-ICA (HSΡΓ-222) to human estrogen receptor protein amplified via the peroxidase-antiperoxidase (P.A.P.) method was used to detect the ER in cryostat, paraffin and acetone sections. In 72 of the 86 studied primary breast cancers, a comparative evaluation was done between the semiquantitative ER-ICA method and the quantitative enzyme immunoassay ER-EIA. In 24 of the total 86 cancers ER-ICA was compared with the biochemical dextran coated charcoal method (D.C.C.). The D.C.C. method was used for the simultaneous PG12 determination. An excellent agreement was found between the semiquantitative ER-ICA’s results in cryostat and paraffin, but not in acetone (AMEX) sections, concerning both, the percentage of positive or negative cells and the staining intensity. There was a linear correlation between the semiquantitative ER-ICA’s results in cryostat and paraffin sections (not in AMEX sections) and the quantitative ER-EIA’s results. Finally, a straight line correlation was observed between ER-EIA and D.C.C. method, regarding the ER but not the PGR determination.","abstract_has_math":false,"creators":["Stylianidou, Artemis","Στυλιανίδου, Άρτεμις"],"institution":"University of Ioannina","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":1990,"date_issued":"1990","date_published":"1990","updated_at":"2026-07-24T02:24:49Z","subjects":["Ανοσοενζυματική μέθοδος","Καρκίνωμα μαστού","Μονοκλωνικά αντισώματα","Προνάση","Τομές ακετόνης","Τομές παραφίνης","Υποδοχείς οιστρογόνων","Χρώση Ανοσοϋπεροξειδάσης","Acetone sections","Breast cancer","Enzyme antibodies","Estrogen receptors","Immunoperoxidase staining","Paraffin sections","Pronase","Ιατρική και Επιστήμες Υγείας","Βασική Ιατρική","Medical and Health Sciences","Basic Medicine"],"languages":["gre"],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["10.12681/eadd/1304"],"render_values":[{"text":"10.12681/eadd/1304","href":"https://doi.org/10.12681/eadd/1304","code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/10442/hedi/1304","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["Stylianidou, Artemis","Στυλιανίδου, Άρτεμις"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["1990"]},{"key":"dc:publisher","label":"Institution","values":["University of Ioannina","Πανεπιστήμιο Ιωαννίνων"]},{"key":"dc:type","label":"Dc Type","values":["PhD Thesis"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Ανοσοενζυματική μέθοδος","Καρκίνωμα μαστού","Μονοκλωνικά αντισώματα","Προνάση","Τομές ακετόνης","Τομές παραφίνης","Υποδοχείς οιστρογόνων","Χρώση Ανοσοϋπεροξειδάσης","Acetone sections","Breast cancer","Enzyme antibodies","Estrogen receptors","Immunoperoxidase staining","Paraffin sections","Pronase","Ιατρική και Επιστήμες Υγείας","Βασική Ιατρική","Medical and Health Sciences","Basic Medicine"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["gre"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["10.12681/eadd/1304","http://hdl.handle.net/10442/hedi/1304"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["The monoclonal antibody ER-ICA (HSΡΓ-222) to human estrogen receptor protein amplified via the peroxidase-antiperoxidase (P.A.P.) method was used to detect the ER in cryostat, paraffin and acetone sections. In 72 of the 86 studied primary breast cancers, a comparative evaluation was done between the semiquantitative ER-ICA method and the quantitative enzyme immunoassay ER-EIA. In 24 of the total 86 cancers ER-ICA was compared with the biochemical dextran coated charcoal method (D.C.C.). The D.C.C. method was used for the simultaneous PG12 determination. An excellent agreement was found between the semiquantitative ER-ICA’s results in cryostat and paraffin, but not in acetone (AMEX) sections, concerning both, the percentage of positive or negative cells and the staining intensity. There was a linear correlation between the semiquantitative ER-ICA’s results in cryostat and paraffin sections (not in AMEX sections) and the quantitative ER-EIA’s results. Finally, a straight line correlation was observed between ER-EIA and D.C.C. method, regarding the ER but not the PGR determination.","Με την χρησιμοποίηση του μονοκλωνικού αντισώματος ER-ICA (HSΡΓ-222) και την μέθοδο της ανοσοϋπεροξειδάσης (P.A.P.) ανιχνεύθηκαν ανοσοϊστοχημικά, οι υποδοχείς οιστρογόνων (ER) σε τομές κρυοστάτη, παραφίνης και ακετόνης (AMEX). Έγινε συγκριτική αξιολόγηση προς την ποσοτική ανοσοενζυματική μέθοδο ER-EIA σε 72 από τα 86 καρκινώματα μαστού και προς την βιοχημική μέθοδο του άνθρακα (D.C.C.) σε 24 απ’ αυτά. Με την τελευταία μέθοδο προσδιορίστηκαν συγχρόνως και οι υποδοχείς προγεστερόνης (PGR). Υπήρχε στατιστικά σημαντική συμφωνία των αποτελεσμάτων, από την εφαρμογή του ER-ICA σε τομές κρυοστάτη και παραφίνης, αλλά όχι στις τομές AMEX. Η συμφωνία αφορούσε τόσο στο ποσοστό των θετικών ή αρνητικών κυττάρων, όσο και στην ένταση της χρωστικής αντίδρασης. Υπήρχε γραμμική συσχέτιση μεταξύ των ημιποσοτικών αποτελεσμάτων του ER-ICA στις τομές κρυοστάτη και παραφίνης και των ποσοτικών αποτελεσμάτων της ER-EIA. H αναλογική αυτή σχέση δεν υπήρχε για τις τομές ακετόνης. Τέλος, γραμμική συνάρτηση παρατηρήθηκε μεταξύ της μεθόδου ER-EIA και D.C.C. όσο αφορά τον προσδιορισμό των ER, αλλά όχι των PGR."]},{"key":"dc:title","label":"Title","values":["Συγκριτική ανοσοϊστοχημική μελέτη υποδοχέων οιστρογόνων καρκίνου μαστού σε τομές κρυοστάτη και παραφίνης προς βιοχημικές και ανοσοενζυματικές μεθόδους","Comparative immunohistochemical study of estrogen receptors using parafin and cryostat sections versus biochemical and enzyme-immuno essays"]}]}],"canonical_facts":{"dc:creator":["Stylianidou, Artemis","Στυλιανίδου, Άρτεμις"],"dc:date":["1990"],"dc:description":["The monoclonal antibody ER-ICA (HSΡΓ-222) to human estrogen receptor protein amplified via the peroxidase-antiperoxidase (P.A.P.) method was used to detect the ER in cryostat, paraffin and acetone sections. In 72 of the 86 studied primary breast cancers, a comparative evaluation was done between the semiquantitative ER-ICA method and the quantitative enzyme immunoassay ER-EIA. In 24 of the total 86 cancers ER-ICA was compared with the biochemical dextran coated charcoal method (D.C.C.). The D.C.C. method was used for the simultaneous PG12 determination. An excellent agreement was found between the semiquantitative ER-ICA’s results in cryostat and paraffin, but not in acetone (AMEX) sections, concerning both, the percentage of positive or negative cells and the staining intensity. There was a linear correlation between the semiquantitative ER-ICA’s results in cryostat and paraffin sections (not in AMEX sections) and the quantitative ER-EIA’s results. Finally, a straight line correlation was observed between ER-EIA and D.C.C. method, regarding the ER but not the PGR determination.","Με την χρησιμοποίηση του μονοκλωνικού αντισώματος ER-ICA (HSΡΓ-222) και την μέθοδο της ανοσοϋπεροξειδάσης (P.A.P.) ανιχνεύθηκαν ανοσοϊστοχημικά, οι υποδοχείς οιστρογόνων (ER) σε τομές κρυοστάτη, παραφίνης και ακετόνης (AMEX). Έγινε συγκριτική αξιολόγηση προς την ποσοτική ανοσοενζυματική μέθοδο ER-EIA σε 72 από τα 86 καρκινώματα μαστού και προς την βιοχημική μέθοδο του άνθρακα (D.C.C.) σε 24 απ’ αυτά. Με την τελευταία μέθοδο προσδιορίστηκαν συγχρόνως και οι υποδοχείς προγεστερόνης (PGR). Υπήρχε στατιστικά σημαντική συμφωνία των αποτελεσμάτων, από την εφαρμογή του ER-ICA σε τομές κρυοστάτη και παραφίνης, αλλά όχι στις τομές AMEX. Η συμφωνία αφορούσε τόσο στο ποσοστό των θετικών ή αρνητικών κυττάρων, όσο και στην ένταση της χρωστικής αντίδρασης. Υπήρχε γραμμική συσχέτιση μεταξύ των ημιποσοτικών αποτελεσμάτων του ER-ICA στις τομές κρυοστάτη και παραφίνης και των ποσοτικών αποτελεσμάτων της ER-EIA. H αναλογική αυτή σχέση δεν υπήρχε για τις τομές ακετόνης. Τέλος, γραμμική συνάρτηση παρατηρήθηκε μεταξύ της μεθόδου ER-EIA και D.C.C. όσο αφορά τον προσδιορισμό των ER, αλλά όχι των PGR."],"dc:identifier":["10.12681/eadd/1304","http://hdl.handle.net/10442/hedi/1304"],"dc:language":["gre"],"dc:publisher":["University of Ioannina","Πανεπιστήμιο Ιωαννίνων"],"dc:subject":["Ανοσοενζυματική μέθοδος","Καρκίνωμα μαστού","Μονοκλωνικά αντισώματα","Προνάση","Τομές ακετόνης","Τομές παραφίνης","Υποδοχείς οιστρογόνων","Χρώση Ανοσοϋπεροξειδάσης","Acetone sections","Breast cancer","Enzyme antibodies","Estrogen receptors","Immunoperoxidase staining","Paraffin sections","Pronase","Ιατρική και Επιστήμες Υγείας","Βασική Ιατρική","Medical and Health Sciences","Basic Medicine"],"dc:title":["Συγκριτική ανοσοϊστοχημική μελέτη υποδοχέων οιστρογόνων καρκίνου μαστού σε τομές κρυοστάτη και παραφίνης προς βιοχημικές και ανοσοενζυματικές μεθόδους","Comparative immunohistochemical study of estrogen receptors using parafin and cryostat sections versus biochemical and enzyme-immuno essays"],"dc:type":["PhD Thesis"]},"updated_at":"2026-07-24T02:24:49Z"}