East Tennessee State University
Localization of Calbindin-D<sub>28k</sub> in Extra-Embryonic Membranes of Two Oviparous Scincid Lizards.
Abstract
dc:description.abstract<p>Calbindin-D<sub>28K</sub> is a cytosolic calcium binding protein found in a variety of cells that transport calcium. The chorioallantoic membrane and yolk sac of oviparous squamate reptiles (lizards and snakes) transport calcium from the eggshell and yolk to the developing embryo. I used immunohistochemistry to localize calbindin-D<sub>28K</sub> expression in the chorioallantoic membrane and yolk sac of two species of oviparous scincid lizards, <em>Plestiodon fasciatus</em> and <em>Saproscincus mustelinus</em>. Calbindin-D<sub>28K</sub> was detected in the chorioallantoic membrane and yolk sac of both lizard species by a polyclonal anti-snake calbindin antibody and a monoclonal anti-cow calbindin antibody. Calbindin-D<sub>28K</sub> was localized in the chorionic epithelium and allantoic epithelium of the chorioallantoic membrane and in endodermal cells scattered throughout the yolk mass of both species. This is the first demonstration of calbindin-D<sub>28K</sub> in allantoic epithelium and in endodermal cells of the yolk sac of lizards.</p>
Degree
thesis:*- Name thesis:degree_name
- MS (Master of Science)
- Level thesis:degree_level
- Thesis - restricted
- Discipline thesis:degree_discipline
- Biomedical Sciences
- Year dc:date.issued
- 2009
Author and committee
dc:creator, dc:contributor.*- Author dc:creator
-
- Li, Shuo
Subjects
dc:subject × 6Rights
dc:rights- Statement dc:rights
-
- Copyright by the authors.
Identifiers
dc:identifier.*- Repository record dc:identifier
- https://dc.etsu.edu/etd/1793
- OAI identifier oai:identifier
- oai:dc.etsu.edu:etd-3145