{"id":{"repo_id":"etsu","oai_identifier":"oai:dc.etsu.edu:etd-3145"},"canonical_url":"https://search.dev.ndltd.org/etd/etsu/oai:dc.etsu.edu:etd-3145","repository":{"repo_id":"etsu","name":"East Tennessee State University","base_url":"https://dc.etsu.edu/do/oai/"},"display":{"title":"Localization of Calbindin-D<sub>28k</sub> in Extra-Embryonic Membranes of Two Oviparous Scincid Lizards.","abstract":"<p>Calbindin-D<sub>28K</sub> is a cytosolic calcium binding protein found in a variety of cells that transport calcium. The chorioallantoic membrane and yolk sac of oviparous squamate reptiles (lizards and snakes) transport calcium from the eggshell and yolk to the developing embryo. I used immunohistochemistry to localize calbindin-D<sub>28K</sub> expression in the chorioallantoic membrane and yolk sac of two species of oviparous scincid lizards, <em>Plestiodon fasciatus</em> and <em>Saproscincus mustelinus</em>. Calbindin-D<sub>28K</sub> was detected in the chorioallantoic membrane and yolk sac of both lizard species by a polyclonal anti-snake calbindin antibody and a monoclonal anti-cow calbindin antibody. Calbindin-D<sub>28K</sub> was localized in the chorionic epithelium and allantoic epithelium of the chorioallantoic membrane and in endodermal cells scattered throughout the yolk mass of both species. This is the first demonstration of calbindin-D<sub>28K</sub> in allantoic epithelium and in endodermal cells of the yolk sac of lizards.</p>","abstract_html":"&lt;p&gt;Calbindin-D&lt;sub&gt;28K&lt;/sub&gt; is a cytosolic calcium binding protein found in a variety of cells that transport calcium. The chorioallantoic membrane and yolk sac of oviparous squamate reptiles (lizards and snakes) transport calcium from the eggshell and yolk to the developing embryo. I used immunohistochemistry to localize calbindin-D&lt;sub&gt;28K&lt;/sub&gt; expression in the chorioallantoic membrane and yolk sac of two species of oviparous scincid lizards, &lt;em&gt;Plestiodon fasciatus&lt;/em&gt; and &lt;em&gt;Saproscincus mustelinus&lt;/em&gt;. Calbindin-D&lt;sub&gt;28K&lt;/sub&gt; was detected in the chorioallantoic membrane and yolk sac of both lizard species by a polyclonal anti-snake calbindin antibody and a monoclonal anti-cow calbindin antibody. Calbindin-D&lt;sub&gt;28K&lt;/sub&gt; was localized in the chorionic epithelium and allantoic epithelium of the chorioallantoic membrane and in endodermal cells scattered throughout the yolk mass of both species. This is the first demonstration of calbindin-D&lt;sub&gt;28K&lt;/sub&gt; in allantoic epithelium and in endodermal cells of the yolk sac of lizards.&lt;/p&gt;","abstract_has_math":false,"creators":["Li, Shuo"],"institution":null,"degree_name":"MS (Master of Science)","degree_level":"Thesis - restricted","degree_discipline":"Biomedical Sciences","degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2009,"date_issued":"2009-08-19T07:00:00Z","date_published":"2009-08-19T07:00:00Z","updated_at":"2026-07-24T02:20:55Z","subjects":["chorioallantoic membrane","calbindin-D28k","yolk sac","Amino Acids, Peptides, and Proteins","Chemicals and Drugs","Medicine and Health Sciences"],"languages":[],"rights":["Copyright by the authors."],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://dc.etsu.edu/etd/1793","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["Li, Shuo"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.issued","label":"Date","values":["2009-08-19T07:00:00Z"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Biomedical Sciences"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Thesis - restricted"]},{"key":"thesis:degree_name","label":"Degree Name","values":["MS (Master of Science)"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["chorioallantoic membrane","calbindin-D28k","yolk sac","Amino Acids, Peptides, and Proteins","Chemicals and Drugs","Medicine and Health Sciences"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:rights","label":"Dc Rights","values":["Copyright by the authors."]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://dc.etsu.edu/context/etd/article/3145/viewcontent/LiS081009f.pdf","https://dc.etsu.edu/etd/1793"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["<p>Calbindin-D<sub>28K</sub> is a cytosolic calcium binding protein found in a variety of cells that transport calcium. The chorioallantoic membrane and yolk sac of oviparous squamate reptiles (lizards and snakes) transport calcium from the eggshell and yolk to the developing embryo. I used immunohistochemistry to localize calbindin-D<sub>28K</sub> expression in the chorioallantoic membrane and yolk sac of two species of oviparous scincid lizards, <em>Plestiodon fasciatus</em> and <em>Saproscincus mustelinus</em>. Calbindin-D<sub>28K</sub> was detected in the chorioallantoic membrane and yolk sac of both lizard species by a polyclonal anti-snake calbindin antibody and a monoclonal anti-cow calbindin antibody. Calbindin-D<sub>28K</sub> was localized in the chorionic epithelium and allantoic epithelium of the chorioallantoic membrane and in endodermal cells scattered throughout the yolk mass of both species. This is the first demonstration of calbindin-D<sub>28K</sub> in allantoic epithelium and in endodermal cells of the yolk sac of lizards.</p>"]},{"key":"dc:title","label":"Title","values":["Localization of Calbindin-D<sub>28k</sub> in Extra-Embryonic Membranes of Two Oviparous Scincid Lizards."]}]}],"canonical_facts":{"dc:creator":["Li, Shuo"],"dc:date.issued":["2009-08-19T07:00:00Z"],"dc:description.abstract":["<p>Calbindin-D<sub>28K</sub> is a cytosolic calcium binding protein found in a variety of cells that transport calcium. The chorioallantoic membrane and yolk sac of oviparous squamate reptiles (lizards and snakes) transport calcium from the eggshell and yolk to the developing embryo. I used immunohistochemistry to localize calbindin-D<sub>28K</sub> expression in the chorioallantoic membrane and yolk sac of two species of oviparous scincid lizards, <em>Plestiodon fasciatus</em> and <em>Saproscincus mustelinus</em>. Calbindin-D<sub>28K</sub> was detected in the chorioallantoic membrane and yolk sac of both lizard species by a polyclonal anti-snake calbindin antibody and a monoclonal anti-cow calbindin antibody. Calbindin-D<sub>28K</sub> was localized in the chorionic epithelium and allantoic epithelium of the chorioallantoic membrane and in endodermal cells scattered throughout the yolk mass of both species. This is the first demonstration of calbindin-D<sub>28K</sub> in allantoic epithelium and in endodermal cells of the yolk sac of lizards.</p>"],"dc:identifier":["https://dc.etsu.edu/context/etd/article/3145/viewcontent/LiS081009f.pdf","https://dc.etsu.edu/etd/1793"],"dc:rights":["Copyright by the authors."],"dc:subject":["chorioallantoic membrane","calbindin-D28k","yolk sac","Amino Acids, Peptides, and Proteins","Chemicals and Drugs","Medicine and Health Sciences"],"dc:title":["Localization of Calbindin-D<sub>28k</sub> in Extra-Embryonic Membranes of Two Oviparous Scincid Lizards."],"thesis:degree_discipline":["Biomedical Sciences"],"thesis:degree_level":["Thesis - restricted"],"thesis:degree_name":["MS (Master of Science)"]},"updated_at":"2026-07-24T02:20:55Z"}