Study of Immobilizing Cadmium Selenide Quantum Dots in Selected Polymers for Application in Peroxyoxalate Chemiluminescence Flow Injection Analysis

<p>Two batches of CdSe QDs with different sizes were synthesized for immobilizing in polyisoprene (PI), polymethylmethacrylate (PMMA), and low-density polyethylene (LDPE). The combinations of QDs and polymer substrates were evaluated for their analytical fit-for-use in applicable immunoassays. Hydrogen peroxide standards were injected into the flow injection analyzer (FIA) constructed to simulate enzyme-generated hydrogen peroxide reacting with bis-(2,4,6-trichlorophenyl) oxalate.</p> <p>Linear correlations between hydrogen peroxide and chemilumenscent intensities yielded regression values greater than 0.9750 for hydrogen peroxide concentrations between 1.0 x 10^-4 M and 1.0 x 10^-1 M. The developed technique’s LOD was approximately 10 ppm. Variability of the prepared QD-polymer products was as low as 3.2% throughout all preparations.Stability of the preparations was tested during a 30-day period that displayed up to a four-fold increase in the first 10 days. The preparations were decently robust to the FIA system demonstrating up to a 15.20% intensity loss after twenty repetitive injections.</p>

Name thesis:degree_name

MS (Master of Science)

Level thesis:degree_level

Thesis - unrestricted

Discipline thesis:degree_discipline

Chemistry

Year dc:date.issued

2013

Author dc:creator

• Moore, Christopher S

• nanochemistry
• quantum dot
• immobilization
• CdSe
• peroxyoxalate
• chemiluminescence
• Analytical Chemistry
• Bioimaging and Biomedical Optics
• Nanoscience and Nanotechnology

Statement dc:rights

• Copyright by the authors.

Repository record dc:identifier

https://dc.etsu.edu/etd/1151

OAI identifier oai:identifier

oai:dc.etsu.edu:etd-2338