East Tennessee State University
Creating Genetic Engineering Tools for Investigating Bacillus anthracis.
Abstract
dc:description.abstract<p><em>Bacillus anthracis</em> is a Gram positive, spore forming, non-motile, rod shaped, soil bacterium, and is endemic worldwide. Currently, the biology of <em>B. anthracis</em> is poorly understood. <em>B. anthracis</em> is one of many biological weapons used today. A -/- mutant strain of <em>B. anthracis</em> that lacks the pathogenic plasmids was created by serial culture at 42°C. Key DNA replication genes were identified by homology as targets. The <em>dnaB</em> gene, essential for <em>B. subtilis</em> initiation of DNA replication, was my focus. A vector system was created by polymerase chain reaction (PCR) with the pMUTIN4 integration vector and the promoter region of <em>dnaB</em> to study the genetics of <em>B. anthracis</em>. An electro-transformation system was formulated to knock-out the -/- <em>B. anthracis</em> <em>dnaB</em> gene. We have successfully incorporated the pMUTIN4 vector into the chromosomal DNA of <em>B. anthracis</em>. We also have formulated an electro-transformation system and vector system for use in <em>B. anthracis</em>.</p>
Degree
thesis:*- Name thesis:degree_name
- MS (Master of Science)
- Level thesis:degree_level
- Thesis - restricted
- Discipline thesis:degree_discipline
- Biomedical Sciences
- Year dc:date.issued
- 2003
Author and committee
dc:creator, dc:contributor.*- Author dc:creator
-
- Anderson, Robert Clayton, III
Subjects
dc:subject × 6Rights
dc:rights- Statement dc:rights
-
- Copyright by the authors.
Identifiers
dc:identifier.*- Repository record dc:identifier
- https://dc.etsu.edu/etd/819
- OAI identifier oai:identifier
- oai:dc.etsu.edu:etd-1976