Bacillus anthracis is a Gram positive, spore forming, non-motile, rod shaped, soil bacterium, and is endemic worldwide. Currently, the biology of B. anthracis is poorly understood. B. anthracis is one of many biological weapons used today. A -/- mutant strain of B. anthracis that lacks the pathogenic plasmids was created by serial culture at 42°C. Key DNA replication genes were identified by homology as targets. The dnaB gene, essential for B. subtilis initiation of DNA replication, was my focus. A vector system was created by polymerase chain reaction (PCR) with the pMUTIN4 integration vector and the promoter region of dnaB to study the genetics of B. anthracis. An electro-transformation system was formulated to knock-out the -/- B. anthracis dnaB gene. We have successfully incorporated the pMUTIN4 vector into the chromosomal DNA of B. anthracis. We also have formulated an electro-transformation system and vector system for use in B. anthracis.
","abstract_html":"<p><em>Bacillus anthracis</em> is a Gram positive, spore forming, non-motile, rod shaped, soil bacterium, and is endemic worldwide. Currently, the biology of <em>B. anthracis</em> is poorly understood. <em>B. anthracis</em> is one of many biological weapons used today. A -/- mutant strain of <em>B. anthracis</em> that lacks the pathogenic plasmids was created by serial culture at 42°C. Key DNA replication genes were identified by homology as targets. The <em>dnaB</em> gene, essential for <em>B. subtilis</em> initiation of DNA replication, was my focus. A vector system was created by polymerase chain reaction (PCR) with the pMUTIN4 integration vector and the promoter region of <em>dnaB</em> to study the genetics of <em>B. anthracis</em>. An electro-transformation system was formulated to knock-out the -/- <em>B. anthracis</em> <em>dnaB</em> gene. We have successfully incorporated the pMUTIN4 vector into the chromosomal DNA of <em>B. anthracis</em>. We also have formulated an electro-transformation system and vector system for use in <em>B. anthracis</em>.</p>","abstract_has_math":false,"creators":["Anderson, Robert Clayton, III"],"institution":null,"degree_name":"MS (Master of Science)","degree_level":"Thesis - restricted","degree_discipline":"Biomedical Sciences","degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2003,"date_issued":"2003-12-01T08:00:00Z","date_published":"2003-12-01T08:00:00Z","updated_at":"2026-07-24T02:19:28Z","subjects":["Genetic Engineering Tools","Bacillus anthracis","dnaB","pMUTIN4","Medical Sciences","Medicine and Health Sciences"],"languages":[],"rights":["Copyright by the authors."],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://dc.etsu.edu/etd/819","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["Anderson, Robert Clayton, III"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.available","label":"Dc Date Available","values":["1990-01-01T08:00:00Z"]},{"key":"dc:date.issued","label":"Date","values":["2003-12-01T08:00:00Z"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Biomedical Sciences"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Thesis - restricted"]},{"key":"thesis:degree_name","label":"Degree Name","values":["MS (Master of Science)"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Genetic Engineering Tools","Bacillus anthracis","dnaB","pMUTIN4","Medical Sciences","Medicine and Health Sciences"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:rights","label":"Dc Rights","values":["Copyright by the authors."]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://dc.etsu.edu/context/etd/article/1976/viewcontent/AndersonR112503f.pdf","https://dc.etsu.edu/etd/819"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Bacillus anthracis is a Gram positive, spore forming, non-motile, rod shaped, soil bacterium, and is endemic worldwide. Currently, the biology of B. anthracis is poorly understood. B. anthracis is one of many biological weapons used today. A -/- mutant strain of B. anthracis that lacks the pathogenic plasmids was created by serial culture at 42°C. Key DNA replication genes were identified by homology as targets. The dnaB gene, essential for B. subtilis initiation of DNA replication, was my focus. A vector system was created by polymerase chain reaction (PCR) with the pMUTIN4 integration vector and the promoter region of dnaB to study the genetics of B. anthracis. An electro-transformation system was formulated to knock-out the -/- B. anthracis dnaB gene. We have successfully incorporated the pMUTIN4 vector into the chromosomal DNA of B. anthracis. We also have formulated an electro-transformation system and vector system for use in B. anthracis.
"]},{"key":"dc:title","label":"Title","values":["Creating Genetic Engineering Tools for Investigating Bacillus anthracis."]}]}],"canonical_facts":{"dc:creator":["Anderson, Robert Clayton, III"],"dc:date.available":["1990-01-01T08:00:00Z"],"dc:date.issued":["2003-12-01T08:00:00Z"],"dc:description.abstract":["Bacillus anthracis is a Gram positive, spore forming, non-motile, rod shaped, soil bacterium, and is endemic worldwide. Currently, the biology of B. anthracis is poorly understood. B. anthracis is one of many biological weapons used today. A -/- mutant strain of B. anthracis that lacks the pathogenic plasmids was created by serial culture at 42°C. Key DNA replication genes were identified by homology as targets. The dnaB gene, essential for B. subtilis initiation of DNA replication, was my focus. A vector system was created by polymerase chain reaction (PCR) with the pMUTIN4 integration vector and the promoter region of dnaB to study the genetics of B. anthracis. An electro-transformation system was formulated to knock-out the -/- B. anthracis dnaB gene. We have successfully incorporated the pMUTIN4 vector into the chromosomal DNA of B. anthracis. We also have formulated an electro-transformation system and vector system for use in B. anthracis.
"],"dc:identifier":["https://dc.etsu.edu/context/etd/article/1976/viewcontent/AndersonR112503f.pdf","https://dc.etsu.edu/etd/819"],"dc:rights":["Copyright by the authors."],"dc:subject":["Genetic Engineering Tools","Bacillus anthracis","dnaB","pMUTIN4","Medical Sciences","Medicine and Health Sciences"],"dc:title":["Creating Genetic Engineering Tools for Investigating Bacillus anthracis."],"thesis:degree_discipline":["Biomedical Sciences"],"thesis:degree_level":["Thesis - restricted"],"thesis:degree_name":["MS (Master of Science)"]},"updated_at":"2026-07-24T02:19:28Z"}