Avaliação da expressão de citoqueratinas na citologia esfoliativa da mucosa bucal de pacientes fumantes

The present study compared the expression of cytokeratins CK6, CK16 and CK19 and pan-cytokeratin (PAN) in oral mucosa cells between smokers and nonsmokers in order to determine the stage of cell differentiation and to consequently infer proliferative activity and expressions indicative of a potential for malignant differentiation. Thirty smokers and 30 non-smokers seen at the clinics of FOSJC-UNESP were screened. Smears were obtained from the left lateral border of the tongue with a cytobrush and slides were processed for immunohistochemistry using the antibodies reported Conventional microscopy was used for qualitative analysis. The results were analyzed statistically by the Z test, Fisher's exact test and comparison of two proportions (plus-4 confidence interval method). The expression of CK6 (p=0.002), CK16 (p=0.003), CK19 (p=0.0001) and PAN (p=0.008) was higher in oral mucosa smears from smokers compared to non- smokers. ln conclusion, increased epithelial proliferation is observed in the oral mucosa of smokers as demonstrated by the increased expression of CK6 and CKJ6, and these cells present alterations in epithelial maturation

Grantor dc:publisher

Universidade Estadual Paulista (Unesp)

Year dc:date.issued

2008

Author dc:creator

• Almeida, Janete Dias [UNESP]

Committee members dc:contributor.committeemember

• Jorge Junior, Jacks
• Cabral, Luiz Antonio Guimarães
• Cavalcante, Ana Sueli Rodrigues
• Mendonça, Elismauro Francisco de
• Migliari, Dante Antonio

• Diagnostico citologico
• Mucosa bucal
• Carcinoma de celulas escamosas
• Fumo - Efeito fisiologico
• Imuno-histoquímica
• Immunohistochemistry

Language dc:language.iso

por

Handle dc:identifier.uri

http://hdl.handle.net/11449/114097

OAI identifier oai:identifier

oai:repositorio.unesp.br:11449/114097