{"id":{"repo_id":"brazil-unesp","oai_identifier":"oai:repositorio.unesp.br:11449/114097"},"canonical_url":"https://search.dev.ndltd.org/etd/brazil-unesp/oai:repositorio.unesp.br:11449/114097","repository":{"repo_id":"brazil-unesp","name":"Brazil UNESP","base_url":"https://repositorio.unesp.br/server/oai/request"},"display":{"title":"Avaliação da expressão de citoqueratinas na citologia esfoliativa da mucosa bucal de pacientes fumantes","abstract":"The present study compared the expression of cytokeratins CK6, CK16 and CK19 and pan-cytokeratin (PAN) in oral mucosa cells between smokers and nonsmokers in order to determine the stage of cell differentiation and to consequently infer proliferative activity and expressions indicative of a potential for malignant differentiation. Thirty smokers and 30 non-smokers seen at the clinics of FOSJC-UNESP were screened. Smears were obtained from the left lateral border of the tongue with a cytobrush and slides were processed for immunohistochemistry using the antibodies reported Conventional microscopy was used for qualitative analysis. The results were analyzed statistically by the Z test, Fisher's exact test and comparison of two proportions (plus-4 confidence interval method). The expression of CK6 (p=0.002), CK16 (p=0.003), CK19 (p=0.0001) and PAN (p=0.008) was higher in oral mucosa smears from smokers compared to non- smokers. ln conclusion, increased epithelial proliferation is observed in the oral mucosa of smokers as demonstrated by the increased expression of CK6 and CKJ6, and these cells present alterations in epithelial maturation","abstract_html":"The present study compared the expression of cytokeratins CK6, CK16 and CK19 and pan-cytokeratin (PAN) in oral mucosa cells between smokers and nonsmokers in order to determine the stage of cell differentiation and to consequently infer proliferative activity and expressions indicative of a potential for malignant differentiation. Thirty smokers and 30 non-smokers seen at the clinics of FOSJC-UNESP were screened. Smears were obtained from the left lateral border of the tongue with a cytobrush and slides were processed for immunohistochemistry using the antibodies reported Conventional microscopy was used for qualitative analysis. The results were analyzed statistically by the Z test, Fisher's exact test and comparison of two proportions (plus-4 confidence interval method). The expression of CK6 (p=0.002), CK16 (p=0.003), CK19 (p=0.0001) and PAN (p=0.008) was higher in oral mucosa smears from smokers compared to non- smokers. ln conclusion, increased epithelial proliferation is observed in the oral mucosa of smokers as demonstrated by the increased expression of CK6 and CKJ6, and these cells present alterations in epithelial maturation","abstract_has_math":false,"creators":["Almeida, Janete Dias [UNESP]"],"institution":"Universidade Estadual Paulista (Unesp)","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":["Jorge Junior, Jacks","Cabral, Luiz Antonio Guimarães","Cavalcante, Ana Sueli Rodrigues","Mendonça, Elismauro Francisco de","Migliari, Dante Antonio"],"year":2008,"date_issued":"2008-06-26","date_published":"2008-06-26","updated_at":"2026-07-27T19:01:14Z","subjects":["Diagnostico citologico","Mucosa bucal","Carcinoma de celulas escamosas","Fumo - Efeito fisiologico","Imuno-histoquímica","Immunohistochemistry"],"languages":["por"],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/11449/114097","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.committeemember","label":"Committee Member","values":["Jorge Junior, Jacks","Cabral, Luiz Antonio Guimarães","Cavalcante, Ana Sueli Rodrigues","Mendonça, Elismauro Francisco de","Migliari, Dante Antonio"]},{"key":"dc:creator","label":"Author","values":["Almeida, Janete Dias [UNESP]"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2015-01-26T13:21:32Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2015-01-26T13:21:32Z"]},{"key":"dc:date.issued","label":"Date","values":["2008-06-26"]},{"key":"dc:publisher","label":"Institution","values":["Universidade Estadual Paulista (Unesp)"]},{"key":"dc:type","label":"Dc Type","values":["Tese de livre-docência"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Diagnostico citologico","Mucosa bucal","Carcinoma de celulas escamosas","Fumo - Efeito fisiologico","Imuno-histoquímica","Immunohistochemistry"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language.iso","label":"Language (ISO)","values":["por"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["http://hdl.handle.net/11449/114097"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["The present study compared the expression of cytokeratins CK6, CK16 and CK19 and pan-cytokeratin (PAN) in oral mucosa cells between smokers and nonsmokers in order to determine the stage of cell differentiation and to consequently infer proliferative activity and expressions indicative of a potential for malignant differentiation. Thirty smokers and 30 non-smokers seen at the clinics of FOSJC-UNESP were screened. Smears were obtained from the left lateral border of the tongue with a cytobrush and slides were processed for immunohistochemistry using the antibodies reported Conventional microscopy was used for qualitative analysis. The results were analyzed statistically by the Z test, Fisher's exact test and comparison of two proportions (plus-4 confidence interval method). The expression of CK6 (p=0.002), CK16 (p=0.003), CK19 (p=0.0001) and PAN (p=0.008) was higher in oral mucosa smears from smokers compared to non- smokers. ln conclusion, increased epithelial proliferation is observed in the oral mucosa of smokers as demonstrated by the increased expression of CK6 and CKJ6, and these cells present alterations in epithelial maturation","O presente trabalho avaliou a expressão das citoqueratinas CK6, CK16, CK19 e pan-citoqueratina em células da mucosa bucal de pacientes fumantes, comparando com células de pacientes não - fumantes, para verificação do estágio de diferenciação celular e inferir conseqüentemente a atividade proliferativa e expressões indicativas de potencial de diferenciação maligna. Foram triados trinta e dois pacientes fumantes e vinte não fumantes, que freqüenta as clínicas da FOSJC-UNESP. Esfregaços da borda lateral esquerda da língua dos pacientes foram realizados utilizando-se o cytobrush e as lâminas obtidas foram processadas pela técnica de imunoistoquímica para os referidos anticorpos. Uma análise qualitativa e quantitativa foi realizada através da microscopia comum e a análise estatística utilizou o Teste Z, teste Exato de Fisher e método de comparação de duas proporções intervalo mais quatro. Foi verificada superioridade de expressão da CK6 (p=0,002), CKI6 (p=0,003), CKI9 (p=0,0001) e PAN (p=0,008), nos esfregaços avaliados da mucosa bucal de pacientes fumantes comparado aos não fumantes. Conclui-se que existe um aumento da proliferação epitelial na mucosa bucal de pacientes fumantes indicada pelo aumento da expressão das CK6 e 16, e que estas células estão sofrendo alterações na maturação epitelial"]},{"key":"dc:source","label":"Dc Source","values":["Aleph"]},{"key":"dc:title","label":"Title","values":["Avaliação da expressão de citoqueratinas na citologia esfoliativa da mucosa bucal de pacientes fumantes"]}]}],"canonical_facts":{"dc:contributor.committeemember":["Jorge Junior, Jacks","Cabral, Luiz Antonio Guimarães","Cavalcante, Ana Sueli Rodrigues","Mendonça, Elismauro Francisco de","Migliari, Dante Antonio"],"dc:creator":["Almeida, Janete Dias [UNESP]"],"dc:date.accessioned":["2015-01-26T13:21:32Z"],"dc:date.available":["2015-01-26T13:21:32Z"],"dc:date.issued":["2008-06-26"],"dc:description.abstract":["The present study compared the expression of cytokeratins CK6, CK16 and CK19 and pan-cytokeratin (PAN) in oral mucosa cells between smokers and nonsmokers in order to determine the stage of cell differentiation and to consequently infer proliferative activity and expressions indicative of a potential for malignant differentiation. Thirty smokers and 30 non-smokers seen at the clinics of FOSJC-UNESP were screened. Smears were obtained from the left lateral border of the tongue with a cytobrush and slides were processed for immunohistochemistry using the antibodies reported Conventional microscopy was used for qualitative analysis. The results were analyzed statistically by the Z test, Fisher's exact test and comparison of two proportions (plus-4 confidence interval method). The expression of CK6 (p=0.002), CK16 (p=0.003), CK19 (p=0.0001) and PAN (p=0.008) was higher in oral mucosa smears from smokers compared to non- smokers. ln conclusion, increased epithelial proliferation is observed in the oral mucosa of smokers as demonstrated by the increased expression of CK6 and CKJ6, and these cells present alterations in epithelial maturation","O presente trabalho avaliou a expressão das citoqueratinas CK6, CK16, CK19 e pan-citoqueratina em células da mucosa bucal de pacientes fumantes, comparando com células de pacientes não - fumantes, para verificação do estágio de diferenciação celular e inferir conseqüentemente a atividade proliferativa e expressões indicativas de potencial de diferenciação maligna. Foram triados trinta e dois pacientes fumantes e vinte não fumantes, que freqüenta as clínicas da FOSJC-UNESP. Esfregaços da borda lateral esquerda da língua dos pacientes foram realizados utilizando-se o cytobrush e as lâminas obtidas foram processadas pela técnica de imunoistoquímica para os referidos anticorpos. Uma análise qualitativa e quantitativa foi realizada através da microscopia comum e a análise estatística utilizou o Teste Z, teste Exato de Fisher e método de comparação de duas proporções intervalo mais quatro. Foi verificada superioridade de expressão da CK6 (p=0,002), CKI6 (p=0,003), CKI9 (p=0,0001) e PAN (p=0,008), nos esfregaços avaliados da mucosa bucal de pacientes fumantes comparado aos não fumantes. Conclui-se que existe um aumento da proliferação epitelial na mucosa bucal de pacientes fumantes indicada pelo aumento da expressão das CK6 e 16, e que estas células estão sofrendo alterações na maturação epitelial"],"dc:identifier.uri":["http://hdl.handle.net/11449/114097"],"dc:language.iso":["por"],"dc:publisher":["Universidade Estadual Paulista (Unesp)"],"dc:source":["Aleph"],"dc:subject":["Diagnostico citologico","Mucosa bucal","Carcinoma de celulas escamosas","Fumo - Efeito fisiologico","Imuno-histoquímica","Immunohistochemistry"],"dc:title":["Avaliação da expressão de citoqueratinas na citologia esfoliativa da mucosa bucal de pacientes fumantes"],"dc:type":["Tese de livre-docência"]},"updated_at":"2026-07-27T19:01:14Z"}