Abstract
dc:descriptionThe aim of the study was to determine bone-regenerative effects of an in situ setting tricalcium phosphate (TCP) cement combined with rhBMP-2 and to compare it with autologous bone graft. A trepanation defect of 1.5 mm in the femur diaphysis of Sprague-Dawley rats was filled with TCP cement combined with 0.25, 2.5, or 25 µg of rhBMP-2. The rats were euthanized after 1 and 3 weeks and the femur was examined by radiography, histology, histomorphometry and bending tests. The TCP-cement showed a close bone-cement contact, it was osteoconductive, biocompatible and resorbable und therewith met important requirements of a carrier for rhBMP-2. Histomorphometric analysis showed no rhBMP-2 dose-dependent increase of new bone formation in the defect. But as shown with intravital fluorochrome staining, new bone formation in the defect started earlier using rhBMP-2. Histomorphometric analysis showed a dose-dependent increase in callus formation when rhBMP-2 was added. This lead to a better stabilitiy in a three-point bending test. Callus formation and bending strength of the TCP + 25 µg rhBMP-2 group was superior to autologous bone graft. TCP/rhBMP-2 composites may prove to be an effective substitute for autologous bone grafts.
Degree
thesis:*- Grantor dc:publisher
- WB Laufersweiler Verl.
- Year dc:date
- 2007
Author and committee
dc:creator, dc:contributor.*- Author dc:creator
-
- Jacob, Eva
- Contributors dc:contributor
-
- Niethard, Fritz Uwe
Subjects
dc:subject × 8Rights
dc:rights- Statement dc:rights
-
- info:eu-repo/semantics/openAccess
- Language dc:language
- ger
Identifiers
dc:identifier.*- OAI identifier oai:identifier
- oai:publications.rwth-aachen.de:62519