{"id":{"repo_id":"aachen","oai_identifier":"oai:publications.rwth-aachen.de:62519"},"canonical_url":"https://search.dev.ndltd.org/etd/aachen/oai:publications.rwth-aachen.de:62519","repository":{"repo_id":"aachen","name":"RWTH Aachen University","base_url":"https://publications.rwth-aachen.de/oai2d"},"display":{"title":"In-vivo-Testung eines neuartigen BMP-2/TCP-Composite","abstract":"The aim of the study was to determine bone-regenerative effects of an in situ setting tricalcium phosphate (TCP) cement combined with rhBMP-2 and to compare it with autologous bone graft. A trepanation defect of 1.5 mm in the femur diaphysis of Sprague-Dawley rats was filled with TCP cement combined with 0.25, 2.5, or 25 µg of rhBMP-2. The rats were euthanized after 1 and 3 weeks and the femur was examined by radiography, histology, histomorphometry and bending tests. The TCP-cement showed a close bone-cement contact, it was osteoconductive, biocompatible and resorbable und therewith met important requirements of a carrier for rhBMP-2. Histomorphometric analysis showed no rhBMP-2 dose-dependent increase of new bone formation in the defect. But as shown with intravital fluorochrome staining, new bone formation in the defect started earlier using rhBMP-2. Histomorphometric analysis showed a dose-dependent increase in callus formation when rhBMP-2 was added. This lead to a better stabilitiy in a three-point bending test. Callus formation and bending strength of the TCP + 25 µg rhBMP-2 group was superior to autologous bone graft. TCP/rhBMP-2 composites may prove to be an effective substitute for autologous bone grafts.","abstract_html":"The aim of the study was to determine bone-regenerative effects of an in situ setting tricalcium phosphate (TCP) cement combined with rhBMP-2 and to compare it with autologous bone graft. A trepanation defect of 1.5 mm in the femur diaphysis of Sprague-Dawley rats was filled with TCP cement combined with 0.25, 2.5, or 25 µg of rhBMP-2. The rats were euthanized after 1 and 3 weeks and the femur was examined by radiography, histology, histomorphometry and bending tests. The TCP-cement showed a close bone-cement contact, it was osteoconductive, biocompatible and resorbable und therewith met important requirements of a carrier for rhBMP-2. Histomorphometric analysis showed no rhBMP-2 dose-dependent increase of new bone formation in the defect. But as shown with intravital fluorochrome staining, new bone formation in the defect started earlier using rhBMP-2. Histomorphometric analysis showed a dose-dependent increase in callus formation when rhBMP-2 was added. This lead to a better stabilitiy in a three-point bending test. Callus formation and bending strength of the TCP + 25 µg rhBMP-2 group was superior to autologous bone graft. 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A trepanation defect of 1.5 mm in the femur diaphysis of Sprague-Dawley rats was filled with TCP cement combined with 0.25, 2.5, or 25 µg of rhBMP-2. The rats were euthanized after 1 and 3 weeks and the femur was examined by radiography, histology, histomorphometry and bending tests. The TCP-cement showed a close bone-cement contact, it was osteoconductive, biocompatible and resorbable und therewith met important requirements of a carrier for rhBMP-2. Histomorphometric analysis showed no rhBMP-2 dose-dependent increase of new bone formation in the defect. But as shown with intravital fluorochrome staining, new bone formation in the defect started earlier using rhBMP-2. Histomorphometric analysis showed a dose-dependent increase in callus formation when rhBMP-2 was added. This lead to a better stabilitiy in a three-point bending test. Callus formation and bending strength of the TCP + 25 µg rhBMP-2 group was superior to autologous bone graft. TCP/rhBMP-2 composites may prove to be an effective substitute for autologous bone grafts."]},{"key":"dc:source","label":"Dc Source","values":["Giessen : WB Laufersweiler Verl. 84 S. : Ill., graph. Darst. (2007). = Zugl.: Aachen, Techn. Hochsch., Diss., 2007"]},{"key":"dc:title","label":"Title","values":["In-vivo-Testung eines neuartigen BMP-2/TCP-Composite"]}]}],"canonical_facts":{"dc:contributor":["Niethard, Fritz Uwe"],"dc:coverage":["DE"],"dc:creator":["Jacob, Eva"],"dc:date":["2007"],"dc:description":["The aim of the study was to determine bone-regenerative effects of an in situ setting tricalcium phosphate (TCP) cement combined with rhBMP-2 and to compare it with autologous bone graft. A trepanation defect of 1.5 mm in the femur diaphysis of Sprague-Dawley rats was filled with TCP cement combined with 0.25, 2.5, or 25 µg of rhBMP-2. The rats were euthanized after 1 and 3 weeks and the femur was examined by radiography, histology, histomorphometry and bending tests. 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