Quantitative und qualitative Analyse der Derivate der Ursodeoxycholsäure im Urin gesunder Probanden

Quantitative and qualitative Analysis of Derivatives of Ursodeoxycholic Acid in urine of healthy human subjects Ursodeoxycholic acid (750 mg daily) was administered orally to ten healthy human subjects over a period of ten days. 24 hour urine samples were collected the day before and on the last day of the study. The samples were extracted with Sep-Pak C18 cartridges and separated into groups of conjugates The following fractions were obtained: unconjugated bile acids, nonamidated bile acid glucosides and N-acetyl-glucosaminides (fraction 1), glycine- or taurine-conjugated bile acids and glycine- or taurine-conjugated bile acid glucosides and N-acetyl-glucosaminides (fraction 2), nonamidated and glycine- or taurine-conjugated bile acid glucuronides (fraction 3) and nonamidated and glycine- or taurine-conjugated bile acid sulfates (fraction 4). Within each fraction the following bile acids were obtained: cholic acid (CA), chenodeoxycholic acid (CDCA), deoxycholic acid (DCA), lithocholic acid (LCA), ursodeoxycholic acid (UDCA) and iso-ursdeoxycholic acid (iso-UDCA). Mean total excretion of urinary bile acids increased from 4,8 µmol/24hr (1,893 µg/24hr) the day before to 15,0 µmol/24hr (5,896 µg/24hr) at day ten of the daily oral administration. At baseline 31,3 % of bile acids were conjugated with glycine or taurine, whereas after oral administration of UDCA N-acetyl-glucosaminides became the most frequent conjugates with a percentage of 33,9 % of the total bile acids. Excretion of N-acetyl-glucosaminides increased from 0,50 µmol/24hr at baseline to 5,10 µmol/24hr on day ten. In total, conjugates with N-acetyl-glucosaminide constituted 55,1 % of all urinary metabolites of UDCA. Iso-UDCA was excreted almost exclusively as N-acetyl-glucosaminide, all other bile acids were not detected among the conjugates with N-acetyl-glucosaminide. Expressed as a percentage of all bile acids UDCA rose from 4 % at baseline to 49 % at day ten, iso-UDCA increased from 3 % to 13 %. Moreover 90 % of UDCA conjugated with N-acetyl-glucosaminide turned out to be double conjugates with glycine. The double conjugates also represented 9 % of iso-UDCA conjugated with N-acetyl-glucosaminide. In the end an intermediate of the epimerization of UDCA to iso-UDCA, 3-dehydro-UDCA, was identified.

Grantor dc:publisher

Publikationsserver der RWTH Aachen University

Year dc:date

2003

Author dc:creator

• Götze, Ulrich Herbert Wilhelm

Contributors dc:contributor

• Marschall, Hanns-Ulrich

• info:eu-repo/classification/ddc/610
• Medizin
• Ursodeoxycholsäure
• N-Acetyl-Glucosaminide
• Gallensäuren

Statement dc:rights

• info:eu-repo/semantics/openAccess

Language dc:language

ger

Identifier

https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-123545%22

OAI identifier oai:identifier

oai:publications.rwth-aachen.de:61936