{"id":{"repo_id":"aachen","oai_identifier":"oai:publications.rwth-aachen.de:61936"},"canonical_url":"https://search.dev.ndltd.org/etd/aachen/oai:publications.rwth-aachen.de:61936","repository":{"repo_id":"aachen","name":"RWTH Aachen University","base_url":"https://publications.rwth-aachen.de/oai2d"},"display":{"title":"Quantitative und qualitative Analyse der Derivate der Ursodeoxycholsäure im Urin gesunder Probanden","abstract":"Quantitative and qualitative Analysis of Derivatives of Ursodeoxycholic Acid in urine of healthy human subjects Ursodeoxycholic acid (750 mg daily) was administered orally to ten healthy human subjects over a period of ten days. 24 hour urine samples were collected the day before and on the last day of the study. The samples were extracted with Sep-Pak C18 cartridges and separated into groups of conjugates The following fractions were obtained: unconjugated bile acids, nonamidated bile acid glucosides and N-acetyl-glucosaminides (fraction 1), glycine- or taurine-conjugated bile acids and glycine- or taurine-conjugated bile acid glucosides and N-acetyl-glucosaminides (fraction 2), nonamidated and glycine- or taurine-conjugated bile acid glucuronides (fraction 3) and nonamidated and glycine- or taurine-conjugated bile acid sulfates (fraction 4). Within each fraction the following bile acids were obtained: cholic acid (CA), chenodeoxycholic acid (CDCA), deoxycholic acid (DCA), lithocholic acid (LCA), ursodeoxycholic acid (UDCA) and iso-ursdeoxycholic acid (iso-UDCA). Mean total excretion of urinary bile acids increased from 4,8 µmol/24hr (1,893 µg/24hr) the day before to 15,0 µmol/24hr (5,896 µg/24hr) at day ten of the daily oral administration. At baseline 31,3 % of bile acids were conjugated with glycine or taurine, whereas after oral administration of UDCA N-acetyl-glucosaminides became the most frequent conjugates with a percentage of 33,9 % of the total bile acids. Excretion of N-acetyl-glucosaminides increased from 0,50 µmol/24hr at baseline to 5,10 µmol/24hr on day ten. In total, conjugates with N-acetyl-glucosaminide constituted 55,1 % of all urinary metabolites of UDCA. Iso-UDCA was excreted almost exclusively as N-acetyl-glucosaminide, all other bile acids were not detected among the conjugates with N-acetyl-glucosaminide. Expressed as a percentage of all bile acids UDCA rose from 4 % at baseline to 49 % at day ten, iso-UDCA increased from 3 % to 13 %. Moreover 90 % of UDCA conjugated with N-acetyl-glucosaminide turned out to be double conjugates with glycine. The double conjugates also represented 9 % of iso-UDCA conjugated with N-acetyl-glucosaminide. In the end an intermediate of the epimerization of UDCA to iso-UDCA, 3-dehydro-UDCA, was identified.","abstract_html":"Quantitative and qualitative Analysis of Derivatives of Ursodeoxycholic Acid in urine of healthy human subjects Ursodeoxycholic acid (750 mg daily) was administered orally to ten healthy human subjects over a period of ten days. 24 hour urine samples were collected the day before and on the last day of the study. The samples were extracted with Sep-Pak C18 cartridges and separated into groups of conjugates The following fractions were obtained: unconjugated bile acids, nonamidated bile acid glucosides and N-acetyl-glucosaminides (fraction 1), glycine- or taurine-conjugated bile acids and glycine- or taurine-conjugated bile acid glucosides and N-acetyl-glucosaminides (fraction 2), nonamidated and glycine- or taurine-conjugated bile acid glucuronides (fraction 3) and nonamidated and glycine- or taurine-conjugated bile acid sulfates (fraction 4). Within each fraction the following bile acids were obtained: cholic acid (CA), chenodeoxycholic acid (CDCA), deoxycholic acid (DCA), lithocholic acid (LCA), ursodeoxycholic acid (UDCA) and iso-ursdeoxycholic acid (iso-UDCA). Mean total excretion of urinary bile acids increased from 4,8 µmol/24hr (1,893 µg/24hr) the day before to 15,0 µmol/24hr (5,896 µg/24hr) at day ten of the daily oral administration. At baseline 31,3 % of bile acids were conjugated with glycine or taurine, whereas after oral administration of UDCA N-acetyl-glucosaminides became the most frequent conjugates with a percentage of 33,9 % of the total bile acids. Excretion of N-acetyl-glucosaminides increased from 0,50 µmol/24hr at baseline to 5,10 µmol/24hr on day ten. In total, conjugates with N-acetyl-glucosaminide constituted 55,1 % of all urinary metabolites of UDCA. Iso-UDCA was excreted almost exclusively as N-acetyl-glucosaminide, all other bile acids were not detected among the conjugates with N-acetyl-glucosaminide. Expressed as a percentage of all bile acids UDCA rose from 4 % at baseline to 49 % at day ten, iso-UDCA increased from 3 % to 13 %. Moreover 90 % of UDCA conjugated with N-acetyl-glucosaminide turned out to be double conjugates with glycine. The double conjugates also represented 9 % of iso-UDCA conjugated with N-acetyl-glucosaminide. In the end an intermediate of the epimerization of UDCA to iso-UDCA, 3-dehydro-UDCA, was identified.","abstract_has_math":false,"creators":["Götze, Ulrich Herbert Wilhelm"],"institution":"Publikationsserver der RWTH Aachen University","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":["Marschall, Hanns-Ulrich"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2003,"date_issued":"2003","date_published":"2003","updated_at":"2026-07-30T19:43:19Z","subjects":["info:eu-repo/classification/ddc/610","Medizin","Ursodeoxycholsäure","N-Acetyl-Glucosaminide","Gallensäuren"],"languages":["ger"],"rights":["info:eu-repo/semantics/openAccess"],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-123545%22"],"render_values":[{"text":"https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-123545%22","href":"https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-123545%22","code":true}]}]},"links":{"outbound_url":"https://publications.rwth-aachen.de/record/61936","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Marschall, Hanns-Ulrich"]},{"key":"dc:creator","label":"Author","values":["Götze, Ulrich Herbert Wilhelm"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:coverage","label":"Dc Coverage","values":["DE"]},{"key":"dc:date","label":"Dc Date","values":["2003"]},{"key":"dc:publisher","label":"Institution","values":["Publikationsserver der RWTH Aachen University"]},{"key":"dc:relation","label":"Dc Relation","values":["info:eu-repo/semantics/altIdentifier/urn/urn:nbn:de:hbz:82-opus-5532"]},{"key":"dc:type","label":"Dc Type","values":["info:eu-repo/semantics/doctoralThesis","info:eu-repo/semantics/publishedVersion"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["info:eu-repo/classification/ddc/610","Medizin","Ursodeoxycholsäure","N-Acetyl-Glucosaminide","Gallensäuren"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["ger"]},{"key":"dc:rights","label":"Dc Rights","values":["info:eu-repo/semantics/openAccess"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://publications.rwth-aachen.de/record/61936","https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-123545%22"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Quantitative and qualitative Analysis of Derivatives of Ursodeoxycholic Acid in urine of healthy human subjects Ursodeoxycholic acid (750 mg daily) was administered orally to ten healthy human subjects over a period of ten days. 24 hour urine samples were collected the day before and on the last day of the study. The samples were extracted with Sep-Pak C18 cartridges and separated into groups of conjugates The following fractions were obtained: unconjugated bile acids, nonamidated bile acid glucosides and N-acetyl-glucosaminides (fraction 1), glycine- or taurine-conjugated bile acids and glycine- or taurine-conjugated bile acid glucosides and N-acetyl-glucosaminides (fraction 2), nonamidated and glycine- or taurine-conjugated bile acid glucuronides (fraction 3) and nonamidated and glycine- or taurine-conjugated bile acid sulfates (fraction 4). Within each fraction the following bile acids were obtained: cholic acid (CA), chenodeoxycholic acid (CDCA), deoxycholic acid (DCA), lithocholic acid (LCA), ursodeoxycholic acid (UDCA) and iso-ursdeoxycholic acid (iso-UDCA). Mean total excretion of urinary bile acids increased from 4,8 µmol/24hr (1,893 µg/24hr) the day before to 15,0 µmol/24hr (5,896 µg/24hr) at day ten of the daily oral administration. At baseline 31,3 % of bile acids were conjugated with glycine or taurine, whereas after oral administration of UDCA N-acetyl-glucosaminides became the most frequent conjugates with a percentage of 33,9 % of the total bile acids. Excretion of N-acetyl-glucosaminides increased from 0,50 µmol/24hr at baseline to 5,10 µmol/24hr on day ten. In total, conjugates with N-acetyl-glucosaminide constituted 55,1 % of all urinary metabolites of UDCA. Iso-UDCA was excreted almost exclusively as N-acetyl-glucosaminide, all other bile acids were not detected among the conjugates with N-acetyl-glucosaminide. Expressed as a percentage of all bile acids UDCA rose from 4 % at baseline to 49 % at day ten, iso-UDCA increased from 3 % to 13 %. Moreover 90 % of UDCA conjugated with N-acetyl-glucosaminide turned out to be double conjugates with glycine. The double conjugates also represented 9 % of iso-UDCA conjugated with N-acetyl-glucosaminide. In the end an intermediate of the epimerization of UDCA to iso-UDCA, 3-dehydro-UDCA, was identified."]},{"key":"dc:source","label":"Dc Source","values":["Aachen : Publikationsserver der RWTH Aachen University 102 S. : graph. Darst. (2003). = Aachen, Techn. Hochsch., Diss., 2003"]},{"key":"dc:title","label":"Title","values":["Quantitative und qualitative Analyse der Derivate der Ursodeoxycholsäure im Urin gesunder Probanden"]}]}],"canonical_facts":{"dc:contributor":["Marschall, Hanns-Ulrich"],"dc:coverage":["DE"],"dc:creator":["Götze, Ulrich Herbert Wilhelm"],"dc:date":["2003"],"dc:description":["Quantitative and qualitative Analysis of Derivatives of Ursodeoxycholic Acid in urine of healthy human subjects Ursodeoxycholic acid (750 mg daily) was administered orally to ten healthy human subjects over a period of ten days. 24 hour urine samples were collected the day before and on the last day of the study. The samples were extracted with Sep-Pak C18 cartridges and separated into groups of conjugates The following fractions were obtained: unconjugated bile acids, nonamidated bile acid glucosides and N-acetyl-glucosaminides (fraction 1), glycine- or taurine-conjugated bile acids and glycine- or taurine-conjugated bile acid glucosides and N-acetyl-glucosaminides (fraction 2), nonamidated and glycine- or taurine-conjugated bile acid glucuronides (fraction 3) and nonamidated and glycine- or taurine-conjugated bile acid sulfates (fraction 4). Within each fraction the following bile acids were obtained: cholic acid (CA), chenodeoxycholic acid (CDCA), deoxycholic acid (DCA), lithocholic acid (LCA), ursodeoxycholic acid (UDCA) and iso-ursdeoxycholic acid (iso-UDCA). Mean total excretion of urinary bile acids increased from 4,8 µmol/24hr (1,893 µg/24hr) the day before to 15,0 µmol/24hr (5,896 µg/24hr) at day ten of the daily oral administration. At baseline 31,3 % of bile acids were conjugated with glycine or taurine, whereas after oral administration of UDCA N-acetyl-glucosaminides became the most frequent conjugates with a percentage of 33,9 % of the total bile acids. Excretion of N-acetyl-glucosaminides increased from 0,50 µmol/24hr at baseline to 5,10 µmol/24hr on day ten. In total, conjugates with N-acetyl-glucosaminide constituted 55,1 % of all urinary metabolites of UDCA. Iso-UDCA was excreted almost exclusively as N-acetyl-glucosaminide, all other bile acids were not detected among the conjugates with N-acetyl-glucosaminide. Expressed as a percentage of all bile acids UDCA rose from 4 % at baseline to 49 % at day ten, iso-UDCA increased from 3 % to 13 %. Moreover 90 % of UDCA conjugated with N-acetyl-glucosaminide turned out to be double conjugates with glycine. The double conjugates also represented 9 % of iso-UDCA conjugated with N-acetyl-glucosaminide. In the end an intermediate of the epimerization of UDCA to iso-UDCA, 3-dehydro-UDCA, was identified."],"dc:identifier":["https://publications.rwth-aachen.de/record/61936","https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-123545%22"],"dc:language":["ger"],"dc:publisher":["Publikationsserver der RWTH Aachen University"],"dc:relation":["info:eu-repo/semantics/altIdentifier/urn/urn:nbn:de:hbz:82-opus-5532"],"dc:rights":["info:eu-repo/semantics/openAccess"],"dc:source":["Aachen : Publikationsserver der RWTH Aachen University 102 S. : graph. Darst. (2003). = Aachen, Techn. Hochsch., Diss., 2003"],"dc:subject":["info:eu-repo/classification/ddc/610","Medizin","Ursodeoxycholsäure","N-Acetyl-Glucosaminide","Gallensäuren"],"dc:title":["Quantitative und qualitative Analyse der Derivate der Ursodeoxycholsäure im Urin gesunder Probanden"],"dc:type":["info:eu-repo/semantics/doctoralThesis","info:eu-repo/semantics/publishedVersion"]},"updated_at":"2026-07-30T19:43:19Z"}