Überexpression von s-Adenosylhomocysteinhydrolase in Epstein-Barr-Virus-positiven Burkitt-Lymphomzellen

The aim of this work was to examine the impact of s-adenosylhomocystein-hydrolase-overexpression on the EVB-positive Burkitt-lymphoma cell line Raji. The focus of the examination was on the morphological and functional characterisation of this overexpression. The AHCY-genproduct was amplified by RT-PCR and cloned into the human expressionsvector pcDNA3.1-E. The successful transformation into this vector was proven by growth of E.coli-TOP10-cells on anibiotica-containing mediums. The insert was identified by sequencing. Raji-cells were transfected with this construct by electroporation and a semiquantitative PCR and a Western Blot showed the successful overexpression of AHCY on transcript and protein levels. As a result the following effects were measured: - the increase of the early antigen (EA) proven by the immunfluorescenz-test (IFT) - the PCR-products of BZLF1 and BHRF1 the first day after transfection - the increase of DNA-synthesis two days after transfection - unchanged growth in comparison to the control cells - an increased expression of CD 19, CD21, CD40 and CD58 - no change in the activity of the examined genes by differential display These results show a way to elucidate the activation of the latent persisting EBV and its signal paths. This could be a step in finding new therapies against EBV-positive tumors.

Grantor dc:publisher

Publikationsserver der RWTH Aachen University

Year dc:date

2003

Author dc:creator

• Maret, Claudine

Contributors dc:contributor

• Ritter, Klaus

• info:eu-repo/classification/ddc/610
• Medizin

Statement dc:rights

• info:eu-repo/semantics/openAccess

Language dc:language

ger

Identifier

https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-121153%22

OAI identifier oai:identifier

oai:publications.rwth-aachen.de:59360