{"id":{"repo_id":"aachen","oai_identifier":"oai:publications.rwth-aachen.de:59360"},"canonical_url":"https://search.dev.ndltd.org/etd/aachen/oai:publications.rwth-aachen.de:59360","repository":{"repo_id":"aachen","name":"RWTH Aachen University","base_url":"https://publications.rwth-aachen.de/oai2d"},"display":{"title":"Überexpression von s-Adenosylhomocysteinhydrolase in Epstein-Barr-Virus-positiven Burkitt-Lymphomzellen","abstract":"The aim of this work was to examine the impact of s-adenosylhomocystein-hydrolase-overexpression on the EVB-positive Burkitt-lymphoma cell line Raji. The focus of the examination was on the morphological and functional characterisation of this overexpression. The AHCY-genproduct was amplified by RT-PCR and cloned into the human expressionsvector pcDNA3.1-E. The successful transformation into this vector was proven by growth of E.coli-TOP10-cells on anibiotica-containing mediums. The insert was identified by sequencing. Raji-cells were transfected with this construct by electroporation and a semiquantitative PCR and a Western Blot showed the successful overexpression of AHCY on transcript and protein levels. As a result the following effects were measured: - the increase of the early antigen (EA) proven by the immunfluorescenz-test (IFT) - the PCR-products of BZLF1 and BHRF1 the first day after transfection - the increase of DNA-synthesis two days after transfection - unchanged growth in comparison to the control cells - an increased expression of CD 19, CD21, CD40 and CD58 - no change in the activity of the examined genes by differential display These results show a way to elucidate the activation of the latent persisting EBV and its signal paths. This could be a step in finding new therapies against EBV-positive tumors.","abstract_html":"The aim of this work was to examine the impact of s-adenosylhomocystein-hydrolase-overexpression on the EVB-positive Burkitt-lymphoma cell line Raji. The focus of the examination was on the morphological and functional characterisation of this overexpression. The AHCY-genproduct was amplified by RT-PCR and cloned into the human expressionsvector pcDNA3.1-E. The successful transformation into this vector was proven by growth of E.coli-TOP10-cells on anibiotica-containing mediums. The insert was identified by sequencing. Raji-cells were transfected with this construct by electroporation and a semiquantitative PCR and a Western Blot showed the successful overexpression of AHCY on transcript and protein levels. As a result the following effects were measured: - the increase of the early antigen (EA) proven by the immunfluorescenz-test (IFT) - the PCR-products of BZLF1 and BHRF1 the first day after transfection - the increase of DNA-synthesis two days after transfection - unchanged growth in comparison to the control cells - an increased expression of CD 19, CD21, CD40 and CD58 - no change in the activity of the examined genes by differential display These results show a way to elucidate the activation of the latent persisting EBV and its signal paths. This could be a step in finding new therapies against EBV-positive tumors.","abstract_has_math":false,"creators":["Maret, Claudine"],"institution":"Publikationsserver der RWTH Aachen University","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":["Ritter, Klaus"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2003,"date_issued":"2003","date_published":"2003","updated_at":"2026-07-30T19:42:39Z","subjects":["info:eu-repo/classification/ddc/610","Medizin"],"languages":["ger"],"rights":["info:eu-repo/semantics/openAccess"],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-121153%22"],"render_values":[{"text":"https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-121153%22","href":"https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-121153%22","code":true}]}]},"links":{"outbound_url":"https://publications.rwth-aachen.de/record/59360","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Ritter, Klaus"]},{"key":"dc:creator","label":"Author","values":["Maret, Claudine"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:coverage","label":"Dc Coverage","values":["DE"]},{"key":"dc:date","label":"Dc Date","values":["2003"]},{"key":"dc:publisher","label":"Institution","values":["Publikationsserver der RWTH Aachen University"]},{"key":"dc:relation","label":"Dc Relation","values":["info:eu-repo/semantics/altIdentifier/urn/urn:nbn:de:hbz:82-opus-7791"]},{"key":"dc:type","label":"Dc Type","values":["info:eu-repo/semantics/doctoralThesis","info:eu-repo/semantics/publishedVersion"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["info:eu-repo/classification/ddc/610","Medizin"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["ger"]},{"key":"dc:rights","label":"Dc Rights","values":["info:eu-repo/semantics/openAccess"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://publications.rwth-aachen.de/record/59360","https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-121153%22"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["The aim of this work was to examine the impact of s-adenosylhomocystein-hydrolase-overexpression on the EVB-positive Burkitt-lymphoma cell line Raji. The focus of the examination was on the morphological and functional characterisation of this overexpression. The AHCY-genproduct was amplified by RT-PCR and cloned into the human expressionsvector pcDNA3.1-E. The successful transformation into this vector was proven by growth of E.coli-TOP10-cells on anibiotica-containing mediums. The insert was identified by sequencing. Raji-cells were transfected with this construct by electroporation and a semiquantitative PCR and a Western Blot showed the successful overexpression of AHCY on transcript and protein levels. As a result the following effects were measured: - the increase of the early antigen (EA) proven by the immunfluorescenz-test (IFT) - the PCR-products of BZLF1 and BHRF1 the first day after transfection - the increase of DNA-synthesis two days after transfection - unchanged growth in comparison to the control cells - an increased expression of CD 19, CD21, CD40 and CD58 - no change in the activity of the examined genes by differential display These results show a way to elucidate the activation of the latent persisting EBV and its signal paths. This could be a step in finding new therapies against EBV-positive tumors."]},{"key":"dc:source","label":"Dc Source","values":["Aachen : Publikationsserver der RWTH Aachen University 88 S., : Ill., graph. Darst. (2003). = Aachen, Techn. Hochsch., Diss., 2003"]},{"key":"dc:title","label":"Title","values":["Überexpression von s-Adenosylhomocysteinhydrolase in Epstein-Barr-Virus-positiven Burkitt-Lymphomzellen"]}]}],"canonical_facts":{"dc:contributor":["Ritter, Klaus"],"dc:coverage":["DE"],"dc:creator":["Maret, Claudine"],"dc:date":["2003"],"dc:description":["The aim of this work was to examine the impact of s-adenosylhomocystein-hydrolase-overexpression on the EVB-positive Burkitt-lymphoma cell line Raji. The focus of the examination was on the morphological and functional characterisation of this overexpression. The AHCY-genproduct was amplified by RT-PCR and cloned into the human expressionsvector pcDNA3.1-E. The successful transformation into this vector was proven by growth of E.coli-TOP10-cells on anibiotica-containing mediums. The insert was identified by sequencing. Raji-cells were transfected with this construct by electroporation and a semiquantitative PCR and a Western Blot showed the successful overexpression of AHCY on transcript and protein levels. As a result the following effects were measured: - the increase of the early antigen (EA) proven by the immunfluorescenz-test (IFT) - the PCR-products of BZLF1 and BHRF1 the first day after transfection - the increase of DNA-synthesis two days after transfection - unchanged growth in comparison to the control cells - an increased expression of CD 19, CD21, CD40 and CD58 - no change in the activity of the examined genes by differential display These results show a way to elucidate the activation of the latent persisting EBV and its signal paths. 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