{"id":{"repo_id":"zurich","oai_identifier":"oai:www.zora.uzh.ch:20.500.14742/221118"},"canonical_url":"https://search.dev.ndltd.org/etd/zurich/oai:www.zora.uzh.ch:20.500.14742/221118","repository":{"repo_id":"zurich","name":"Universität Zurich","base_url":"https://www.zora.uzh.ch/server/oai/request"},"display":{"title":"Sources of Pulmonary GM-CSF and Its Roles in Myeloid Cell Homeostasis","abstract":"Granulocyte-macrophage colony-stimulating factor (GM-CSF, encoded by Csf2) is a pro-inflammatory cytokine essential for the development, maintenance, and maturation of specific myeloid cells. In the lung, GM-CSF serves as a critical local environmental cue that defines the identity of tissue-resident macrophages. Utilizing newly developed transgenic Csf2-reporter mice, we aimed to profile GM-CSF expression in various barrier tissues, including the lung and skin. In the lung, we detected Csf2-reporter expression in immune cells such as group 2 innate lymphoid cells and γδ T cells, as well as in surfactant-secreting alveolar type 2 epithelial cells (AT2s). Lineage-specific constitutive and inducible Csf2 deletion underscored the indispensable role of AT2-derived GM-CSF in directing alveolar macrophage (AM) fate, establishing the postnatal AM compartment, and sustaining AMs in adult lungs. We observed that, unlike other barrier tissues, non-hematopoietic Csf2 expression in the lung was associated with AT2 identity. Although AT2-derived GM-CSF was crucial for supporting pulmonary conventional dendritic cells (cDCs), their dependence on it was less stringent compared to AMs, and could be partially compensated by hematopoietic sources of GM-CSF. In contrast, Csf2 expression in the skin and colon was predominantly observed in distinct lymphoid compartments, such as ILC2s and γδ T cells. Overall, our studies reveal that diverse epithelial and lymphoid sources of GM-CSF differentially regulate the homeostasis of tissue myeloid cells in a tissue-specific manner.","abstract_html":"Granulocyte-macrophage colony-stimulating factor (GM-CSF, encoded by Csf2) is a pro-inflammatory cytokine essential for the development, maintenance, and maturation of specific myeloid cells. In the lung, GM-CSF serves as a critical local environmental cue that defines the identity of tissue-resident macrophages. Utilizing newly developed transgenic Csf2-reporter mice, we aimed to profile GM-CSF expression in various barrier tissues, including the lung and skin. In the lung, we detected Csf2-reporter expression in immune cells such as group 2 innate lymphoid cells and γδ T cells, as well as in surfactant-secreting alveolar type 2 epithelial cells (AT2s). Lineage-specific constitutive and inducible Csf2 deletion underscored the indispensable role of AT2-derived GM-CSF in directing alveolar macrophage (AM) fate, establishing the postnatal AM compartment, and sustaining AMs in adult lungs. We observed that, unlike other barrier tissues, non-hematopoietic Csf2 expression in the lung was associated with AT2 identity. Although AT2-derived GM-CSF was crucial for supporting pulmonary conventional dendritic cells (cDCs), their dependence on it was less stringent compared to AMs, and could be partially compensated by hematopoietic sources of GM-CSF. In contrast, Csf2 expression in the skin and colon was predominantly observed in distinct lymphoid compartments, such as ILC2s and γδ T cells. Overall, our studies reveal that diverse epithelial and lymphoid sources of GM-CSF differentially regulate the homeostasis of tissue myeloid cells in a tissue-specific manner.","abstract_has_math":false,"creators":["Gschwend, Julia"],"institution":null,"degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2024,"date_issued":"2024-09-02","date_published":"2024-09-02","updated_at":"2026-08-21T16:51:05Z","subjects":["610 Medicine & health","570 Life sciences; biology"],"languages":["eng"],"rights":["info:eu-repo/semantics/openAccess"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":null,"outbound_label":null,"outbound_source":null},"source_record":{"url":"https://www.zora.uzh.ch/server/oai/request?verb=GetRecord&metadataPrefix=oai_dc&identifier=oai%3Awww.zora.uzh.ch%3A20.500.14742%2F221118","prefix":"oai_dc"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["Gschwend, Julia"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2024-09-02"]},{"key":"dc:relation","label":"Dc Relation","values":["https://www.zora.uzh.ch/handle/20.500.14742/221118"]},{"key":"dc:type","label":"Dc Type","values":["Dissertation"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["610 Medicine & health","570 Life sciences; biology"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]},{"key":"dc:rights","label":"Dc Rights","values":["info:eu-repo/semantics/openAccess"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Granulocyte-macrophage colony-stimulating factor (GM-CSF, encoded by Csf2) is a pro-inflammatory cytokine essential for the development, maintenance, and maturation of specific myeloid cells. In the lung, GM-CSF serves as a critical local environmental cue that defines the identity of tissue-resident macrophages. Utilizing newly developed transgenic Csf2-reporter mice, we aimed to profile GM-CSF expression in various barrier tissues, including the lung and skin. In the lung, we detected Csf2-reporter expression in immune cells such as group 2 innate lymphoid cells and γδ T cells, as well as in surfactant-secreting alveolar type 2 epithelial cells (AT2s). Lineage-specific constitutive and inducible Csf2 deletion underscored the indispensable role of AT2-derived GM-CSF in directing alveolar macrophage (AM) fate, establishing the postnatal AM compartment, and sustaining AMs in adult lungs. We observed that, unlike other barrier tissues, non-hematopoietic Csf2 expression in the lung was associated with AT2 identity. Although AT2-derived GM-CSF was crucial for supporting pulmonary conventional dendritic cells (cDCs), their dependence on it was less stringent compared to AMs, and could be partially compensated by hematopoietic sources of GM-CSF. In contrast, Csf2 expression in the skin and colon was predominantly observed in distinct lymphoid compartments, such as ILC2s and γδ T cells. Overall, our studies reveal that diverse epithelial and lymphoid sources of GM-CSF differentially regulate the homeostasis of tissue myeloid cells in a tissue-specific manner."]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:source","label":"Dc Source","values":["Gschwend, J. (2024). Sources of Pulmonary GM-CSF and Its Roles in Myeloid Cell Homeostasis. (Dissertation, University of Zurich) https://doi.org/10.5167/uzh-262037"]},{"key":"dc:title","label":"Title","values":["Sources of Pulmonary GM-CSF and Its Roles in Myeloid Cell Homeostasis"]}]}],"canonical_facts":{"dc:creator":["Gschwend, Julia"],"dc:date":["2024-09-02"],"dc:description":["Granulocyte-macrophage colony-stimulating factor (GM-CSF, encoded by Csf2) is a pro-inflammatory cytokine essential for the development, maintenance, and maturation of specific myeloid cells. In the lung, GM-CSF serves as a critical local environmental cue that defines the identity of tissue-resident macrophages. Utilizing newly developed transgenic Csf2-reporter mice, we aimed to profile GM-CSF expression in various barrier tissues, including the lung and skin. In the lung, we detected Csf2-reporter expression in immune cells such as group 2 innate lymphoid cells and γδ T cells, as well as in surfactant-secreting alveolar type 2 epithelial cells (AT2s). Lineage-specific constitutive and inducible Csf2 deletion underscored the indispensable role of AT2-derived GM-CSF in directing alveolar macrophage (AM) fate, establishing the postnatal AM compartment, and sustaining AMs in adult lungs. We observed that, unlike other barrier tissues, non-hematopoietic Csf2 expression in the lung was associated with AT2 identity. Although AT2-derived GM-CSF was crucial for supporting pulmonary conventional dendritic cells (cDCs), their dependence on it was less stringent compared to AMs, and could be partially compensated by hematopoietic sources of GM-CSF. In contrast, Csf2 expression in the skin and colon was predominantly observed in distinct lymphoid compartments, such as ILC2s and γδ T cells. Overall, our studies reveal that diverse epithelial and lymphoid sources of GM-CSF differentially regulate the homeostasis of tissue myeloid cells in a tissue-specific manner."],"dc:format":["application/pdf"],"dc:language":["eng"],"dc:relation":["https://www.zora.uzh.ch/handle/20.500.14742/221118"],"dc:rights":["info:eu-repo/semantics/openAccess"],"dc:source":["Gschwend, J. (2024). Sources of Pulmonary GM-CSF and Its Roles in Myeloid Cell Homeostasis. (Dissertation, University of Zurich) https://doi.org/10.5167/uzh-262037"],"dc:subject":["610 Medicine & health","570 Life sciences; biology"],"dc:title":["Sources of Pulmonary GM-CSF and Its Roles in Myeloid Cell Homeostasis"],"dc:type":["Dissertation"]},"updated_at":"2026-08-21T16:51:05Z"}