An in vitro study of the effects of free fatty acids on insulin mediated glucose uptake and metabolism by myocytes and fibroblast derived adipocytes
type: CreativeWork
description: The incidence of type 2 diabetes (T2D) is increasing at an alarming rate, especially in developing countries, including South Africa. In developing countries, changes in lifestyle, especially the increase in the consumption of a westernized diet, rich in fats and sugar, is associated with hyperlipidemia, obesity and development of T2D. Skeletal muscle and adipose tissues are the major tissues involved with post-prandial peripheral glucose disposal in response to insulin. Insulin resistance reduces the ability to clear glucose from the circulation resulting in hyperinsulinemia and hyperglycemia with the development of T2D. High levels of plasma free fatty acids (FFAs), particulary saturated FFAs such as palmitate, are associated with insulin resistance in muscle and adipose tissue. Monounsaturated FFAs, such as oleate and essential FFAs such as omega 3 and omega 6 are claimed to be beneficial and to improve insulin sensitivity. Aim This study aims to determine the in vitro effect of the free fatty acids (FFAs) palmitate, oleate, omega 3 and omega 6 on insulin-stimulated glucose metabolism in myocytes and adipocytes. Materials and methods Mouse C2C12 and rat L8 cells were differentiated into myocytes and myotubules by serum deprivation, while mouse 3T3-L1 fibroblasts were differentiated into adipocytes by culturing in 3-isobutyl-1-methylxanthine (IBMX), dexamenthasone and insulin. Myocytes and adpipocytes were cultured in DMEM containing 5.5 mM or 20 mM glucose supplemented with 0.75 mM of each of the respective FFAs (i.e. palmitate, oleate, omega 3 and omega 6) for 24 hours. Thereafter media was replaced with fresh media containing 0.75 mM of each of the FFAs with or without insulin stimulation in the last hour. Glucose uptake was measured using a 2-deoxy-[3H]-D-glucose method. Glycogen and glucose-6-phosphate (G6P) concentrations were determined using commercial kits. Glucose oxidation was measured by 14CO2 release from cells incubated with glucose D-[14C(U)]. Cell …
abstract: The incidence of type 2 diabetes (T2D) is increasing at an alarming rate, especially in developing countries, including South Africa. In developing countries, changes in lifestyle, especially the increase in the consumption of a westernized diet, rich in fats and sugar, is associated with hyperlipidemia, obesity and development of T2D. Skeletal muscle and adipose tissues are the major tissues involved with post-prandial peripheral glucose disposal in response to insulin. Insulin resistance reduces the ability to clear glucose from the circulation resulting in hyperinsulinemia and hyperglycemia with the development of T2D. High levels of plasma free fatty acids (FFAs), particulary saturated FFAs such as palmitate, are associated with insulin resistance in muscle and adipose tissue. Monounsaturated FFAs, such as oleate and essential FFAs such as omega 3 and omega 6 are claimed to be beneficial and to improve insulin sensitivity. Aim This study aims to determine the in vitro effect of the free fatty acids (FFAs) palmitate, oleate, omega 3 and omega 6 on insulin-stimulated glucose metabolism in myocytes and adipocytes. Materials and methods Mouse C2C12 and rat L8 cells were differentiated into myocytes and myotubules by serum deprivation, while mouse 3T3-L1 fibroblasts were differentiated into adipocytes by culturing in 3-isobutyl-1-methylxanthine (IBMX), dexamenthasone and insulin. Myocytes and adpipocytes were cultured in DMEM containing 5.5 mM or 20 mM glucose supplemented with 0.75 mM of each of the respective FFAs (i.e. palmitate, oleate, omega 3 and omega 6) for 24 hours. Thereafter media was replaced with fresh media containing 0.75 mM of each of the FFAs with or without insulin stimulation in the last hour. Glucose uptake was measured using a 2-deoxy-[3H]-D-glucose method. Glycogen and glucose-6-phosphate (G6P) concentrations were determined using commercial kits. Glucose oxidation was measured by 14CO2 release from cells incubated with glucose D-[14C(U)]. Cell …
author: Mazibuko, Sithandiwe Eunice
keywords: Diabetes -- South Africa, Ffree fatty acids, Insulin-stimulated glucose
license: https://devspace.unizulu.ac.za/bitstreams/c1dd0d66-e262-4cd4-ad05-642edf5a8443/download
date published: 2011
date modified: 2026-07-24T06:17:11Z
provider: University of Zululand
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: University of Zululand