Therapeutic Effect of a triterpenic Acid from Protorhus longifolia Against High Fat Diet Fed and Streptozotocin-induced Diabetic Nephropathy in Rats
type: CreativeWork
description: Diabetic nephropathy (DN) is the major and most frequent complication of diabetes mellitus that contributes to high rate of morbidity and mortality worldwide. Despite the current available treatment regimes, DN still remains the main leading cause of end stage kidney disease. Therefore, more focus is currently placed on the search for alternatively new and effective therapeutic agents, preferably of plant origin. Thus, the present study is aimed at evaluating the therapeutic potential of a plant-derived lanosteryl triterpene (RA5), against high fat diet (HFD) fed and streptozotocin (STZ)- induced diabetic nephropathy in rats. RA5 was isolated from the chloroform extract of Protorhus longifolia stem bark. Chemical structure of RA5 was confirmed based spectral data analysis. The in vitro antioxidant activity of the compound was assessed against DPPH and nitric oxide (NO•) radicals. Its reducing power and metal ion chelating potential were also assessed. The bovine serum albumin (BSA) and haemoglobin fructose assays were used to evaluate theanti- protein glycation activity of RA5. The in vitro inhibitory potential of RA5 against xanthine oxidase (XOD) and angiotensin converting enzyme (ACE) activity was also investigated. The antihyperglycemic potential of RA5 was first established and confirmed in the STZ-induced diabetes rat model. Its therapeutic potential against diabetic nephropathy was then investigated in the HFD and STZ induced diabetic nephropathy in rats. In the STZ (60 mg/kg)-induced diabetes in the rat model, the diabetic animals were treated with RA5 (100 mg/kg) for 14 days and the oral glucose tolerance of the rats was determined. In the HFD fed and STZ induced diabetic nephropathy in rat model, the HFD fed (hyperlipidaemic) rats received an intraperitoneal injection of a low dose of STZ (30 mg/kg) to induce diabetes. The diabetic rats were treated orally with RA5 at 100 mg/kg, daily for 28 days. Metformin (100 mg/kg) and 2% Tween 20 were used as positive…
abstract: Diabetic nephropathy (DN) is the major and most frequent complication of diabetes mellitus that contributes to high rate of morbidity and mortality worldwide. Despite the current available treatment regimes, DN still remains the main leading cause of end stage kidney disease. Therefore, more focus is currently placed on the search for alternatively new and effective therapeutic agents, preferably of plant origin. Thus, the present study is aimed at evaluating the therapeutic potential of a plant-derived lanosteryl triterpene (RA5), against high fat diet (HFD) fed and streptozotocin (STZ)- induced diabetic nephropathy in rats. RA5 was isolated from the chloroform extract of Protorhus longifolia stem bark. Chemical structure of RA5 was confirmed based spectral data analysis. The in vitro antioxidant activity of the compound was assessed against DPPH and nitric oxide (NO•) radicals. Its reducing power and metal ion chelating potential were also assessed. The bovine serum albumin (BSA) and haemoglobin fructose assays were used to evaluate theanti- protein glycation activity of RA5. The in vitro inhibitory potential of RA5 against xanthine oxidase (XOD) and angiotensin converting enzyme (ACE) activity was also investigated. The antihyperglycemic potential of RA5 was first established and confirmed in the STZ-induced diabetes rat model. Its therapeutic potential against diabetic nephropathy was then investigated in the HFD and STZ induced diabetic nephropathy in rats. In the STZ (60 mg/kg)-induced diabetes in the rat model, the diabetic animals were treated with RA5 (100 mg/kg) for 14 days and the oral glucose tolerance of the rats was determined. In the HFD fed and STZ induced diabetic nephropathy in rat model, the HFD fed (hyperlipidaemic) rats received an intraperitoneal injection of a low dose of STZ (30 mg/kg) to induce diabetes. The diabetic rats were treated orally with RA5 at 100 mg/kg, daily for 28 days. Metformin (100 mg/kg) and 2% Tween 20 were used as positive…
author: Mngomezulu, Thembekile S.K.
keywords: Diabetic nephropathy (DN), triterpenic Acid, Protorhus longifolia, High Fat Diet
license: https://devspace.unizulu.ac.za/bitstreams/d2f4ff78-a315-4cb5-91b3-cd581ca916f4/download
date published: 2019
date modified: 2026-07-24T06:16:44Z
provider: University of Zululand
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: University of Zululand