Anti-asthmatic and anti-cough activities of the essential oil Of Eucalyptus Grandis W. Hill Ex Maiden
type: CreativeWork
description: Asthma is a chronic inflammatory disorder of the airways. It is characterized by an inflammation of the airways causing airway dysfunction. Asthma is associated with widespread airflow obstruction, with an associated increase in airway responsiveness to a variety of stimuli. An asthma attack is accompanied by wheezing, shortness of breath, chest tightness and coughing. This project aims to investigate the essential oil of Eucalyptus grandis, a medicinal plant used by Zulu traditional healers for its antiasthmatic and anti-cough activities in the treatment of respiratory tract infections. The anti-asthmatic and anti-cough activities of the essential oils and 1, 8-cineole on rats were assessed. These activities were induced and challenged with histamine and acetylcholine using an ultrasonic nebulizer for asthma and exposure to ammonia for coughs. The assessment of the chemical composition of the essential oils hydrodistilled from the fresh and dry leaves of Eucalyptus grandis was carried out using a GC and GC-MS analysis. Column chromatography was used to isolate 1,8-cineole and terpinen- 4-ol components of the essential oils. Agar well diffusion was used to access antibacterial (Klebsiella pneumoniae, Staphylococcus aureus and Moraxella catarrhalis) susceptibility to the essential oil. Cytosolic LDH was released and efflux pump inhibition activity was monitored to determine the apparent bactericidal mechanism of the essential oils. Antioxidant activity (free radical scavenging of nitric oxide, hydroxyl radical, superoxide anion, and also the sulfhydryl, NADH as well as the malondialdehyde (MDA)—TBARS contents) was determined. vi Anti-inflammatory activities of the essential oils and 1,8-cineole were determined using the cotton pellet granuloma test. Biochemical estimates were carried out on the catalase activity, superoxide dismutase, in vitro COX-1 and COX-2 inhibition assay and the acetylcholinesterase inhibitory activity. Muscle contraction studies where carried o…
abstract: Asthma is a chronic inflammatory disorder of the airways. It is characterized by an inflammation of the airways causing airway dysfunction. Asthma is associated with widespread airflow obstruction, with an associated increase in airway responsiveness to a variety of stimuli. An asthma attack is accompanied by wheezing, shortness of breath, chest tightness and coughing. This project aims to investigate the essential oil of Eucalyptus grandis, a medicinal plant used by Zulu traditional healers for its antiasthmatic and anti-cough activities in the treatment of respiratory tract infections. The anti-asthmatic and anti-cough activities of the essential oils and 1, 8-cineole on rats were assessed. These activities were induced and challenged with histamine and acetylcholine using an ultrasonic nebulizer for asthma and exposure to ammonia for coughs. The assessment of the chemical composition of the essential oils hydrodistilled from the fresh and dry leaves of Eucalyptus grandis was carried out using a GC and GC-MS analysis. Column chromatography was used to isolate 1,8-cineole and terpinen- 4-ol components of the essential oils. Agar well diffusion was used to access antibacterial (Klebsiella pneumoniae, Staphylococcus aureus and Moraxella catarrhalis) susceptibility to the essential oil. Cytosolic LDH was released and efflux pump inhibition activity was monitored to determine the apparent bactericidal mechanism of the essential oils. Antioxidant activity (free radical scavenging of nitric oxide, hydroxyl radical, superoxide anion, and also the sulfhydryl, NADH as well as the malondialdehyde (MDA)—TBARS contents) was determined. vi Anti-inflammatory activities of the essential oils and 1,8-cineole were determined using the cotton pellet granuloma test. Biochemical estimates were carried out on the catalase activity, superoxide dismutase, in vitro COX-1 and COX-2 inhibition assay and the acetylcholinesterase inhibitory activity. Muscle contraction studies where carried o…
author: Soyingbe, Oluwagbemiga Sewanu
keywords: asthma --cough activities --essential oils --Eucalyptus Grandis W. Hill Ex Maiden
license: https://devspace.unizulu.ac.za/bitstreams/304834b2-9fe1-4382-b382-a707fa9700a4/download
date published: 2015
date modified: 2026-07-24T06:16:44Z
provider: University of Zululand
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: University of Zululand