Chemo-morphological characterisation and some biological activities of pelargonium sidoides DC.
type: CreativeWork
description: Pelargonium sidoides DC (Geraniaceae) is one of several medicinal plants indigenous to South Africa. Various concoctions prepared from the plant are used for the treatment of tuberculosis, diarrhea, gonorrhea and fevers. The aim of this study was to evaluate the variability in qualitative and quantitative traits in P. sidoides accessions, correlate different morphotypes with the phytochemical content, and to the antimicrobial activity of P. sidoides extracts. Morphological characterisation of accessions of P. sidoides was done using IPGRI/IITA/BAMBNET list for Bambara groundnut. Principal Component Analysis (PCA) and Cluster Analysis (CA) were used to evaluate the morphological variability and to reveal the groups of different morphotypes. The PCA revealed that the first three principal components exhibited Eigenvalues greater than 1 and explained 74.170% of the total variability, contributing the entire variable to the morphological variation of the accessions established at the University of Zululand. Cluster analysis was able to group the morphotypes into two major groups with each group having two sub-groups. Nine groups of the morphotypes were selected and screened for coumarins (umckalin, esculin and scopoletin) using Thin Layer Chromatography (TLC) and High Performance Liquid Chromatography (HPLC). Coumarins are known to exhibit moderate antibacterial and significant immunomodulatory capabilities. Variation was observed in the phytochemical content (umckalin) of morphotypes of P. sidoides. The content of umckalin varied between 13.90 mg/ml and 4.41 mg/ml in the roots of morphotypes of P. sidoides and 0.15mg/ml and 3.90 mg/ml in the leaves of morphotypes of P. sidoides. Methanolic extracts from leaves and roots of selected morphotypes were screened for antimicrobial activity against Escherichia coli ATCC 8739, Staphylococcus aureus ATTC 6538, Shigella flexineri and Salmonella ssp. The antimicrobial activity was determined by agar-well diffusion method. The min…
abstract: Pelargonium sidoides DC (Geraniaceae) is one of several medicinal plants indigenous to South Africa. Various concoctions prepared from the plant are used for the treatment of tuberculosis, diarrhea, gonorrhea and fevers. The aim of this study was to evaluate the variability in qualitative and quantitative traits in P. sidoides accessions, correlate different morphotypes with the phytochemical content, and to the antimicrobial activity of P. sidoides extracts. Morphological characterisation of accessions of P. sidoides was done using IPGRI/IITA/BAMBNET list for Bambara groundnut. Principal Component Analysis (PCA) and Cluster Analysis (CA) were used to evaluate the morphological variability and to reveal the groups of different morphotypes. The PCA revealed that the first three principal components exhibited Eigenvalues greater than 1 and explained 74.170% of the total variability, contributing the entire variable to the morphological variation of the accessions established at the University of Zululand. Cluster analysis was able to group the morphotypes into two major groups with each group having two sub-groups. Nine groups of the morphotypes were selected and screened for coumarins (umckalin, esculin and scopoletin) using Thin Layer Chromatography (TLC) and High Performance Liquid Chromatography (HPLC). Coumarins are known to exhibit moderate antibacterial and significant immunomodulatory capabilities. Variation was observed in the phytochemical content (umckalin) of morphotypes of P. sidoides. The content of umckalin varied between 13.90 mg/ml and 4.41 mg/ml in the roots of morphotypes of P. sidoides and 0.15mg/ml and 3.90 mg/ml in the leaves of morphotypes of P. sidoides. Methanolic extracts from leaves and roots of selected morphotypes were screened for antimicrobial activity against Escherichia coli ATCC 8739, Staphylococcus aureus ATTC 6538, Shigella flexineri and Salmonella ssp. The antimicrobial activity was determined by agar-well diffusion method. The min…
author: Mthiyane, Pretty Gqamile
keywords: Morphological characterisation, Indigenous plants -- South Africa, Medicinal plants -- South Africa
license: https://devspace.unizulu.ac.za/bitstreams/2b0dbdee-6da8-4e8d-932c-36158d0f8586/download
date published: 2013
date modified: 2026-07-24T06:17:15Z
provider: University of Zululand
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: University of Zululand