Comparative Studies of Escherichia Coli and Salmonela Servars isolated from Kafue Lechwe and Pastrol Cattle in the Interface Areas of the Kafue Flats of Zambia
type: CreativeWork
description: This study was conducted at Lochinvar and Blue Lagoon National Parks in the interface areas of wildlife and livestock of the Kafue Flats of Zambia to establish the prevalence and relatedness of Escherichia colt and Salmonella serogroups isolated from Kafue Lechwe (Kobus leche kafuensis) and pastoral cattle. A total of 593 faecal samples were processed of which 232 (39.1%) were from Kafue lechwe while 361 (60.8%) were from pastoral cattle. Seventy-seven (33.2%) of 232 samples were compound faecal contents from rectum (R), ceacum (C), small intestine (SI) and the ileo-ceacal junction(ICJ) of each carcass, while 155 (66.8%) were faecal droppings conveniently picked from the grazing pastures. Altogether, there were 1283 presumptive E. coll isolates from Kafue lechwe and pastoral cattle out of which 248 (19.3%) had growth characteristics as Shiga toxin-producing E. coli O157 (STEC) on sorbitol MacConkey agar and CHROMagar O157. Of 248 suspected STEC isolates, 104 (41.9%) were from Kafue lechwe while 144 (58.1%) were from pastoral cattle. Only 18 out of 104 (17.3%) E. coli isolates from Kafue lechwe were serogrouped into 8 serogroups while E. coli isolates from pastoral cattle were typed into 16 serogroups. In case of Salmonella, 59 isolates from Kafue lechwe were identified on biochemical and physiological characterization, of which 39 were from Lochinvar NP, while 20 were from Blue-lagoon NP. Nineteen (32.2%) were confirmed on serological typing with Salmonella somatic polyvalent antisera, while 40 (67.8%) were non-typeable. Furthermore, of the 19 Salmonella isolates, 5 (26.3%) were identified to belong to serogroup 8 serovars while the rest did not react to the available Salmonella antisera groups. Thirty suspected Salmonella isolates were isolated and biochemically identified from faecal samples of the pastoral cattle, out of which 13 (43.3%) tested positive on agglutination test with Salmonella polyvalent antisera while the rest of the isolates were non-typeable. Onl…
abstract: This study was conducted at Lochinvar and Blue Lagoon National Parks in the interface areas of wildlife and livestock of the Kafue Flats of Zambia to establish the prevalence and relatedness of Escherichia colt and Salmonella serogroups isolated from Kafue Lechwe (Kobus leche kafuensis) and pastoral cattle. A total of 593 faecal samples were processed of which 232 (39.1%) were from Kafue lechwe while 361 (60.8%) were from pastoral cattle. Seventy-seven (33.2%) of 232 samples were compound faecal contents from rectum (R), ceacum (C), small intestine (SI) and the ileo-ceacal junction(ICJ) of each carcass, while 155 (66.8%) were faecal droppings conveniently picked from the grazing pastures. Altogether, there were 1283 presumptive E. coll isolates from Kafue lechwe and pastoral cattle out of which 248 (19.3%) had growth characteristics as Shiga toxin-producing E. coli O157 (STEC) on sorbitol MacConkey agar and CHROMagar O157. Of 248 suspected STEC isolates, 104 (41.9%) were from Kafue lechwe while 144 (58.1%) were from pastoral cattle. Only 18 out of 104 (17.3%) E. coli isolates from Kafue lechwe were serogrouped into 8 serogroups while E. coli isolates from pastoral cattle were typed into 16 serogroups. In case of Salmonella, 59 isolates from Kafue lechwe were identified on biochemical and physiological characterization, of which 39 were from Lochinvar NP, while 20 were from Blue-lagoon NP. Nineteen (32.2%) were confirmed on serological typing with Salmonella somatic polyvalent antisera, while 40 (67.8%) were non-typeable. Furthermore, of the 19 Salmonella isolates, 5 (26.3%) were identified to belong to serogroup 8 serovars while the rest did not react to the available Salmonella antisera groups. Thirty suspected Salmonella isolates were isolated and biochemically identified from faecal samples of the pastoral cattle, out of which 13 (43.3%) tested positive on agglutination test with Salmonella polyvalent antisera while the rest of the isolates were non-typeable. Onl…
author: Mubita, Charles Miyanda
language: en
keywords: Escherihia coli infections in animals--kafue flats--Zambia, Bacteria diseases in Animals--Zambia
date published: 2012-12-20
date modified: 2026-07-27T22:06:24Z
provider: University of Zambia
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: University of Zambia