{"id":{"repo_id":"york","oai_identifier":"oai:yorkspace.library.yorku.ca:10315/40736"},"canonical_url":"https://search.dev.ndltd.org/etd/york/oai:yorkspace.library.yorku.ca:10315/40736","repository":{"repo_id":"york","name":"York University","base_url":"https://yorkspace.library.yorku.ca/oai/request"},"display":{"title":"Examining the Impact of PUFAs and the Role of Autophagy in Iron-Induced Ferroptotic Cell Death","abstract":"Ferroptosis, an iron-dependent non-apoptotic cell death involves lipid peroxidation and associates with cardiometabolic disease and cancer. I established a model of iron overload in L6 cells and measured cell viability, toxicity and lipid peroxidation. Importantly, in PUFA pre-treated cells, the susceptibility to iron-induced lipid peroxidation was enhanced. This resulted in ferroptosis as cell death was attenuated by ferrostatin-1, a ferroptosis inhibitor, yet no attenuation was seen when an inhibitor of apoptosis was used. Increased lipid peroxidation was also found in PUFA pre-treaded cells in wild type but not in autophagy deficient cells. Additionally, preliminary data indicated an apparent rescue of ferroptosis-induced cell death when using the adiponectin receptor agonist ALY688, an autophagy inducer. In conclusion, these findings supported the theory that PUFAs can alter the cell susceptibility to iron-induced ferroptotic cell death and raise the possibility that autophagy plays a protective role against ferroptosis.","abstract_html":"Ferroptosis, an iron-dependent non-apoptotic cell death involves lipid peroxidation and associates with cardiometabolic disease and cancer. I established a model of iron overload in L6 cells and measured cell viability, toxicity and lipid peroxidation. Importantly, in PUFA pre-treated cells, the susceptibility to iron-induced lipid peroxidation was enhanced. This resulted in ferroptosis as cell death was attenuated by ferrostatin-1, a ferroptosis inhibitor, yet no attenuation was seen when an inhibitor of apoptosis was used. Increased lipid peroxidation was also found in PUFA pre-treaded cells in wild type but not in autophagy deficient cells. Additionally, preliminary data indicated an apparent rescue of ferroptosis-induced cell death when using the adiponectin receptor agonist ALY688, an autophagy inducer. In conclusion, these findings supported the theory that PUFAs can alter the cell susceptibility to iron-induced ferroptotic cell death and raise the possibility that autophagy plays a protective role against ferroptosis.","abstract_has_math":false,"creators":["De Menezes Reboucas, Patricia"],"institution":null,"degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":["Sweeney, Gary"],"committee_chairs":[],"committee_members":[],"year":2022,"date_issued":"2022-12-14","date_published":"2022-12-14","updated_at":"2026-07-24T06:34:00Z","subjects":["Molecular biology","Biology","Cellular biology"],"languages":["en"],"rights":["Author owns copyright, except where explicitly noted. Please contact the author directly with licensing requests."],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/10315/40736","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Sweeney, Gary"]},{"key":"dc:creator","label":"Author","values":["De Menezes Reboucas, Patricia"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2022-12-14T16:37:32Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2022-12-14T16:37:32Z"]},{"key":"dc:date.issued","label":"Date","values":["2022-12-14"]},{"key":"dc:type","label":"Dc Type","values":["Electronic Thesis or Dissertation"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Molecular biology","Biology","Cellular biology"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]},{"key":"dc:rights","label":"Dc Rights","values":["Author owns copyright, except where explicitly noted. Please contact the author directly with licensing requests."]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["http://hdl.handle.net/10315/40736"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Ferroptosis, an iron-dependent non-apoptotic cell death involves lipid peroxidation and associates with cardiometabolic disease and cancer. I established a model of iron overload in L6 cells and measured cell viability, toxicity and lipid peroxidation. Importantly, in PUFA pre-treated cells, the susceptibility to iron-induced lipid peroxidation was enhanced. This resulted in ferroptosis as cell death was attenuated by ferrostatin-1, a ferroptosis inhibitor, yet no attenuation was seen when an inhibitor of apoptosis was used. Increased lipid peroxidation was also found in PUFA pre-treaded cells in wild type but not in autophagy deficient cells. Additionally, preliminary data indicated an apparent rescue of ferroptosis-induced cell death when using the adiponectin receptor agonist ALY688, an autophagy inducer. In conclusion, these findings supported the theory that PUFAs can alter the cell susceptibility to iron-induced ferroptotic cell death and raise the possibility that autophagy plays a protective role against ferroptosis."]},{"key":"dc:title","label":"Title","values":["Examining the Impact of PUFAs and the Role of Autophagy in Iron-Induced Ferroptotic Cell Death"]}]}],"canonical_facts":{"dc:contributor.advisor":["Sweeney, Gary"],"dc:creator":["De Menezes Reboucas, Patricia"],"dc:date.accessioned":["2022-12-14T16:37:32Z"],"dc:date.available":["2022-12-14T16:37:32Z"],"dc:date.issued":["2022-12-14"],"dc:description.abstract":["Ferroptosis, an iron-dependent non-apoptotic cell death involves lipid peroxidation and associates with cardiometabolic disease and cancer. I established a model of iron overload in L6 cells and measured cell viability, toxicity and lipid peroxidation. Importantly, in PUFA pre-treated cells, the susceptibility to iron-induced lipid peroxidation was enhanced. This resulted in ferroptosis as cell death was attenuated by ferrostatin-1, a ferroptosis inhibitor, yet no attenuation was seen when an inhibitor of apoptosis was used. Increased lipid peroxidation was also found in PUFA pre-treaded cells in wild type but not in autophagy deficient cells. Additionally, preliminary data indicated an apparent rescue of ferroptosis-induced cell death when using the adiponectin receptor agonist ALY688, an autophagy inducer. In conclusion, these findings supported the theory that PUFAs can alter the cell susceptibility to iron-induced ferroptotic cell death and raise the possibility that autophagy plays a protective role against ferroptosis."],"dc:identifier.uri":["http://hdl.handle.net/10315/40736"],"dc:language":["en"],"dc:rights":["Author owns copyright, except where explicitly noted. Please contact the author directly with licensing requests."],"dc:subject":["Molecular biology","Biology","Cellular biology"],"dc:title":["Examining the Impact of PUFAs and the Role of Autophagy in Iron-Induced Ferroptotic Cell Death"],"dc:type":["Electronic Thesis or Dissertation"]},"updated_at":"2026-07-24T06:34:00Z"}