{"id":{"repo_id":"york","oai_identifier":"oai:yorkspace.library.yorku.ca:10315/40692"},"canonical_url":"https://search.dev.ndltd.org/etd/york/oai:yorkspace.library.yorku.ca:10315/40692","repository":{"repo_id":"york","name":"York University","base_url":"https://yorkspace.library.yorku.ca/oai/request"},"display":{"title":"Biochemical Studies of EA22, A Protein Involved in The Bacteriophage Lysogenic-Lytic Decision and the Virulence of Clinically Relevant Serotypes of E. Coli","abstract":"Enterohemorrhagic Escherichia coli (EHEC) infections are associated with short term gastrointestinal problems, but in some cases, it can lead to more life-threatening conditions. The pathogenicity of EHEC Is supported by an endogenous, dormant bacteriophage that persists in the bacterium as a lysogen until it is activated by a stress-related event. While the lysogenic-lytic development decision has been characterized for decades, there are still genes that are unknown in every way, from structure to function. These genes are found in a region between exo and xis in the l bacteriophage genome which may serve as a model for understanding other related phages in EHEC. This thesis focuses on the protein encoded by the ea22 gene, the largest conserved gene in the exo-xis region. The most significant finding of my research was the expression and characterization of several Ea22 proteins and protein fragments from l and two phages associated with EHEC. Structural differences in the C-terminal domains of these proteins may help explain the broad range of effects the Ea22 proteins mediate during infection.","abstract_html":"Enterohemorrhagic Escherichia coli (EHEC) infections are associated with short term gastrointestinal problems, but in some cases, it can lead to more life-threatening conditions. The pathogenicity of EHEC Is supported by an endogenous, dormant bacteriophage that persists in the bacterium as a lysogen until it is activated by a stress-related event. While the lysogenic-lytic development decision has been characterized for decades, there are still genes that are unknown in every way, from structure to function. These genes are found in a region between exo and xis in the l bacteriophage genome which may serve as a model for understanding other related phages in EHEC. This thesis focuses on the protein encoded by the ea22 gene, the largest conserved gene in the exo-xis region. The most significant finding of my research was the expression and characterization of several Ea22 proteins and protein fragments from l and two phages associated with EHEC. Structural differences in the C-terminal domains of these proteins may help explain the broad range of effects the Ea22 proteins mediate during infection.","abstract_has_math":false,"creators":["Tong, Jinge"],"institution":null,"degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":["Donaldson, Logan"],"committee_chairs":[],"committee_members":[],"year":2022,"date_issued":"2022-12-14","date_published":"2022-12-14","updated_at":"2026-07-24T06:33:53Z","subjects":["Biology"],"languages":["en"],"rights":["Author owns copyright, except where explicitly noted. Please contact the author directly with licensing requests."],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/10315/40692","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Donaldson, Logan"]},{"key":"dc:creator","label":"Author","values":["Tong, Jinge"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2022-12-14T16:30:40Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2022-12-14T16:30:40Z"]},{"key":"dc:date.issued","label":"Date","values":["2022-12-14"]},{"key":"dc:type","label":"Dc Type","values":["Electronic Thesis or Dissertation"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Biology"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]},{"key":"dc:rights","label":"Dc Rights","values":["Author owns copyright, except where explicitly noted. Please contact the author directly with licensing requests."]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["http://hdl.handle.net/10315/40692"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Enterohemorrhagic Escherichia coli (EHEC) infections are associated with short term gastrointestinal problems, but in some cases, it can lead to more life-threatening conditions. The pathogenicity of EHEC Is supported by an endogenous, dormant bacteriophage that persists in the bacterium as a lysogen until it is activated by a stress-related event. While the lysogenic-lytic development decision has been characterized for decades, there are still genes that are unknown in every way, from structure to function. These genes are found in a region between exo and xis in the l bacteriophage genome which may serve as a model for understanding other related phages in EHEC. This thesis focuses on the protein encoded by the ea22 gene, the largest conserved gene in the exo-xis region. The most significant finding of my research was the expression and characterization of several Ea22 proteins and protein fragments from l and two phages associated with EHEC. Structural differences in the C-terminal domains of these proteins may help explain the broad range of effects the Ea22 proteins mediate during infection."]},{"key":"dc:title","label":"Title","values":["Biochemical Studies of EA22, A Protein Involved in The Bacteriophage Lysogenic-Lytic Decision and the Virulence of Clinically Relevant Serotypes of E. Coli"]}]}],"canonical_facts":{"dc:contributor.advisor":["Donaldson, Logan"],"dc:creator":["Tong, Jinge"],"dc:date.accessioned":["2022-12-14T16:30:40Z"],"dc:date.available":["2022-12-14T16:30:40Z"],"dc:date.issued":["2022-12-14"],"dc:description.abstract":["Enterohemorrhagic Escherichia coli (EHEC) infections are associated with short term gastrointestinal problems, but in some cases, it can lead to more life-threatening conditions. The pathogenicity of EHEC Is supported by an endogenous, dormant bacteriophage that persists in the bacterium as a lysogen until it is activated by a stress-related event. While the lysogenic-lytic development decision has been characterized for decades, there are still genes that are unknown in every way, from structure to function. These genes are found in a region between exo and xis in the l bacteriophage genome which may serve as a model for understanding other related phages in EHEC. This thesis focuses on the protein encoded by the ea22 gene, the largest conserved gene in the exo-xis region. The most significant finding of my research was the expression and characterization of several Ea22 proteins and protein fragments from l and two phages associated with EHEC. Structural differences in the C-terminal domains of these proteins may help explain the broad range of effects the Ea22 proteins mediate during infection."],"dc:identifier.uri":["http://hdl.handle.net/10315/40692"],"dc:language":["en"],"dc:rights":["Author owns copyright, except where explicitly noted. Please contact the author directly with licensing requests."],"dc:subject":["Biology"],"dc:title":["Biochemical Studies of EA22, A Protein Involved in The Bacteriophage Lysogenic-Lytic Decision and the Virulence of Clinically Relevant Serotypes of E. Coli"],"dc:type":["Electronic Thesis or Dissertation"]},"updated_at":"2026-07-24T06:33:53Z"}