{"id":{"repo_id":"york","oai_identifier":"oai:yorkspace.library.yorku.ca:10315/27621"},"canonical_url":"https://search.dev.ndltd.org/etd/york/oai:yorkspace.library.yorku.ca:10315/27621","repository":{"repo_id":"york","name":"York University","base_url":"https://yorkspace.library.yorku.ca/oai/request"},"display":{"title":"Characterization of PRD-1 Mutation in Neurospora crassa","abstract":"In Neurospora crassa, rhythmic conidiation is controlled by several oscillators such as FRQ/WCC and FLO. The frequency (frq) gene and white collar genes (WC-1 and WC-2) are the most important components of the FRQ/WCC oscillator and prd-1 and prd-2 genes could be important components of the FLO. This project aims to characterize the prd-1 mutation. This involved mapping the mutation using PCR analyses based on single nucleotide polymorphism (SNP) markers. After determining a minimal interval, candidate genes were sequenced, and knockout mutants were screened in this interval for prd-1 phenotype. A candidate gene was found to have a mutation that affects the splicing of the mRNA. The identity of the gene was confirmed by complementing the prd-1 mutant with a wild type copy of the identified candidate gene. The prd-1 gene is identified as an RNA helicase.","abstract_html":"In Neurospora crassa, rhythmic conidiation is controlled by several oscillators such as FRQ/WCC and FLO. The frequency (frq) gene and white collar genes (WC-1 and WC-2) are the most important components of the FRQ/WCC oscillator and prd-1 and prd-2 genes could be important components of the FLO. This project aims to characterize the prd-1 mutation. This involved mapping the mutation using PCR analyses based on single nucleotide polymorphism (SNP) markers. After determining a minimal interval, candidate genes were sequenced, and knockout mutants were screened in this interval for prd-1 phenotype. A candidate gene was found to have a mutation that affects the splicing of the mRNA. The identity of the gene was confirmed by complementing the prd-1 mutant with a wild type copy of the identified candidate gene. The prd-1 gene is identified as an RNA helicase.","abstract_has_math":false,"creators":["Firoozi, Ghazaleh"],"institution":null,"degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":["Lakin-Thomas, Patricia"],"committee_chairs":[],"committee_members":[],"year":2014,"date_issued":"2014-07-09","date_published":"2014-07-09","updated_at":"2026-07-24T06:33:40Z","subjects":["Biology"],"languages":["en"],"rights":["Author owns copyright, except where explicitly noted. Please contact the author directly with licensing requests."],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/10315/27621","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Lakin-Thomas, Patricia"]},{"key":"dc:creator","label":"Author","values":["Firoozi, Ghazaleh"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2014-07-14T20:57:32Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2014-07-14T20:57:32Z"]},{"key":"dc:date.issued","label":"Date","values":["2014-07-09"]},{"key":"dc:type","label":"Dc Type","values":["Electronic Thesis or Dissertation"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Biology"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language.iso","label":"Language (ISO)","values":["en"]},{"key":"dc:rights","label":"Dc Rights","values":["Author owns copyright, except where explicitly noted. Please contact the author directly with licensing requests."]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["http://hdl.handle.net/10315/27621"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["In Neurospora crassa, rhythmic conidiation is controlled by several oscillators such as FRQ/WCC and FLO. The frequency (frq) gene and white collar genes (WC-1 and WC-2) are the most important components of the FRQ/WCC oscillator and prd-1 and prd-2 genes could be important components of the FLO. This project aims to characterize the prd-1 mutation. This involved mapping the mutation using PCR analyses based on single nucleotide polymorphism (SNP) markers. After determining a minimal interval, candidate genes were sequenced, and knockout mutants were screened in this interval for prd-1 phenotype. A candidate gene was found to have a mutation that affects the splicing of the mRNA. The identity of the gene was confirmed by complementing the prd-1 mutant with a wild type copy of the identified candidate gene. The prd-1 gene is identified as an RNA helicase."]},{"key":"dc:title","label":"Title","values":["Characterization of PRD-1 Mutation in Neurospora crassa"]}]}],"canonical_facts":{"dc:contributor.advisor":["Lakin-Thomas, Patricia"],"dc:creator":["Firoozi, Ghazaleh"],"dc:date.accessioned":["2014-07-14T20:57:32Z"],"dc:date.available":["2014-07-14T20:57:32Z"],"dc:date.issued":["2014-07-09"],"dc:description.abstract":["In Neurospora crassa, rhythmic conidiation is controlled by several oscillators such as FRQ/WCC and FLO. The frequency (frq) gene and white collar genes (WC-1 and WC-2) are the most important components of the FRQ/WCC oscillator and prd-1 and prd-2 genes could be important components of the FLO. This project aims to characterize the prd-1 mutation. This involved mapping the mutation using PCR analyses based on single nucleotide polymorphism (SNP) markers. After determining a minimal interval, candidate genes were sequenced, and knockout mutants were screened in this interval for prd-1 phenotype. A candidate gene was found to have a mutation that affects the splicing of the mRNA. The identity of the gene was confirmed by complementing the prd-1 mutant with a wild type copy of the identified candidate gene. The prd-1 gene is identified as an RNA helicase."],"dc:identifier.uri":["http://hdl.handle.net/10315/27621"],"dc:language.iso":["en"],"dc:rights":["Author owns copyright, except where explicitly noted. Please contact the author directly with licensing requests."],"dc:subject":["Biology"],"dc:title":["Characterization of PRD-1 Mutation in Neurospora crassa"],"dc:type":["Electronic Thesis or Dissertation"]},"updated_at":"2026-07-24T06:33:40Z"}