{"id":{"repo_id":"wvu","oai_identifier":"oai:researchrepository.wvu.edu:etd-2564"},"canonical_url":"https://search.dev.ndltd.org/etd/wvu/oai:researchrepository.wvu.edu:etd-2564","repository":{"repo_id":"wvu","name":"West Virginia University","base_url":"https://researchrepository.wvu.edu/do/oai/"},"display":{"title":"Characterization of metallothionein expression in response to cadmium in the 267-B1, normal prostate cell line","abstract":"The objective of the study was to characterize the mRNA and protein expression of the MT gene in response to Cd2+ exposure in the 267-B1 immortalized normal human neonatal prostate cell line. The cells were exposed to 18muM Cd2+ for 4hr and rescued for 48hr in P4-8F normal medium. Samples were obtained at 0, 1, 2, 4hr exposure and 4, 8, 12, 16, 24, 36, and 48hr recovery. Basal levels of mRNA for MT-1E, MT-1X, and MT-2A genes in 267-B1 cells were similar to the expression of the G3PDH where as MT-3 had a low basal level expression. Exposure to Cd2+ following recovery in normal medium showed the expression of MT-1A, MT-1F, MT-1G genes and a 3 fold induction of MT-1E and a lesser induction of MT-1X and MT-2A. The basal level of MT-1/2 protein was found to be 3.2 +/- 0.17 ng MT/total protein with levels gradually increasing as a result of Cd2+ exposure. No MT-3 protein was induced on exposure to Cd2+.","abstract_html":"The objective of the study was to characterize the mRNA and protein expression of the MT gene in response to Cd2+ exposure in the 267-B1 immortalized normal human neonatal prostate cell line. The cells were exposed to 18muM Cd2+ for 4hr and rescued for 48hr in P4-8F normal medium. Samples were obtained at 0, 1, 2, 4hr exposure and 4, 8, 12, 16, 24, 36, and 48hr recovery. Basal levels of mRNA for MT-1E, MT-1X, and MT-2A genes in 267-B1 cells were similar to the expression of the G3PDH where as MT-3 had a low basal level expression. Exposure to Cd2+ following recovery in normal medium showed the expression of MT-1A, MT-1F, MT-1G genes and a 3 fold induction of MT-1E and a lesser induction of MT-1X and MT-2A. The basal level of MT-1/2 protein was found to be 3.2 +/- 0.17 ng MT/total protein with levels gradually increasing as a result of Cd2+ exposure. No MT-3 protein was induced on exposure to Cd2+.","abstract_has_math":false,"creators":["Neriyanuri, Madhuri G."],"institution":null,"degree_name":"MS","degree_level":"Thesis","degree_discipline":"Physiology, Pharmacology & Neuroscience","degree_department":null,"school":null,"contributors":["Donald A. Sens","Joginder Nath"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2002,"date_issued":"2002-12-01T08:00:00Z","date_published":"2002-12-01T08:00:00Z","updated_at":"2026-07-24T06:15:55Z","subjects":["Genetics","Animal Physiology"],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["https://researchrepository.wvu.edu/etd/1561"],"render_values":[{"text":"https://researchrepository.wvu.edu/etd/1561","href":"https://researchrepository.wvu.edu/etd/1561","code":true}]}]},"links":{"outbound_url":"https://doi.org/10.33915/etd.1561","outbound_label":"DOI","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Donald A. Sens","Joginder Nath"]},{"key":"dc:creator","label":"Author","values":["Neriyanuri, Madhuri G."]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.available","label":"Dc Date Available","values":["2019-01-17T08:00:00Z"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Physiology, Pharmacology & Neuroscience"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["MS"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Genetics","Animal Physiology"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://doi.org/10.33915/etd.1561","https://researchrepository.wvu.edu/etd/1561"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["The objective of the study was to characterize the mRNA and protein expression of the MT gene in response to Cd2+ exposure in the 267-B1 immortalized normal human neonatal prostate cell line. The cells were exposed to 18muM Cd2+ for 4hr and rescued for 48hr in P4-8F normal medium. Samples were obtained at 0, 1, 2, 4hr exposure and 4, 8, 12, 16, 24, 36, and 48hr recovery. Basal levels of mRNA for MT-1E, MT-1X, and MT-2A genes in 267-B1 cells were similar to the expression of the G3PDH where as MT-3 had a low basal level expression. Exposure to Cd2+ following recovery in normal medium showed the expression of MT-1A, MT-1F, MT-1G genes and a 3 fold induction of MT-1E and a lesser induction of MT-1X and MT-2A. The basal level of MT-1/2 protein was found to be 3.2 +/- 0.17 ng MT/total protein with levels gradually increasing as a result of Cd2+ exposure. No MT-3 protein was induced on exposure to Cd2+."]},{"key":"dc:title","label":"Title","values":["Characterization of metallothionein expression in response to cadmium in the 267-B1, normal prostate cell line"]}]}],"canonical_facts":{"dc:contributor":["Donald A. Sens","Joginder Nath"],"dc:creator":["Neriyanuri, Madhuri G."],"dc:date.available":["2019-01-17T08:00:00Z"],"dc:description.abstract":["The objective of the study was to characterize the mRNA and protein expression of the MT gene in response to Cd2+ exposure in the 267-B1 immortalized normal human neonatal prostate cell line. The cells were exposed to 18muM Cd2+ for 4hr and rescued for 48hr in P4-8F normal medium. Samples were obtained at 0, 1, 2, 4hr exposure and 4, 8, 12, 16, 24, 36, and 48hr recovery. Basal levels of mRNA for MT-1E, MT-1X, and MT-2A genes in 267-B1 cells were similar to the expression of the G3PDH where as MT-3 had a low basal level expression. Exposure to Cd2+ following recovery in normal medium showed the expression of MT-1A, MT-1F, MT-1G genes and a 3 fold induction of MT-1E and a lesser induction of MT-1X and MT-2A. The basal level of MT-1/2 protein was found to be 3.2 +/- 0.17 ng MT/total protein with levels gradually increasing as a result of Cd2+ exposure. No MT-3 protein was induced on exposure to Cd2+."],"dc:identifier":["https://doi.org/10.33915/etd.1561","https://researchrepository.wvu.edu/etd/1561"],"dc:subject":["Genetics","Animal Physiology"],"dc:title":["Characterization of metallothionein expression in response to cadmium in the 267-B1, normal prostate cell line"],"thesis:degree_discipline":["Physiology, Pharmacology & Neuroscience"],"thesis:degree_level":["Thesis"],"thesis:degree_name":["MS"]},"updated_at":"2026-07-24T06:15:55Z"}