{"id":{"repo_id":"wvu","oai_identifier":"oai:researchrepository.wvu.edu:etd-2522"},"canonical_url":"https://search.dev.ndltd.org/etd/wvu/oai:researchrepository.wvu.edu:etd-2522","repository":{"repo_id":"wvu","name":"West Virginia University","base_url":"https://researchrepository.wvu.edu/do/oai/"},"display":{"title":"Effect of peptidoglycan-polysaccharide complex on reproductive efficiency and mastitis in sheep","abstract":"Bacterial infections associated with mastitis reduce pregnancies in cattle. Effects on pregnancy and incidence of mastitis in sheep were investigated after immunization with peptidoglycan-polysaccharide (PG-PS) and killed cells from Strep. pyogenes. Rambouillet (n = 100) and mixed breed (n = 18) ewe lambs were immunized (&sim;d42 and 22 before breeding) with PG-PS (30mugPG/kg/bw) or killed cells or were not immunized (Control, n = 117). IgG antibodies were detected by ELISA. Ewes were bred at synchronized estrus. All immunized and half of non-immunized ewes were challenged with PG-PS (60mugPG/kg/bw) d5 after breeding. Although proportion of ewes pregnant at d42 did not differ, probability of pregnancy decreased with total dose of PG-PS (p < .05). Incidence of mastitis in mixed breed ewes in the middle and at the end of lactation did not differ. In conclusion, immunization of ewe lambs with PG-PS or killed cells of Strep. pyogenes did not improve pregnancy rate or incidence of mastitis after PG-PS challenge.","abstract_html":"Bacterial infections associated with mastitis reduce pregnancies in cattle. Effects on pregnancy and incidence of mastitis in sheep were investigated after immunization with peptidoglycan-polysaccharide (PG-PS) and killed cells from Strep. pyogenes. Rambouillet (n = 100) and mixed breed (n = 18) ewe lambs were immunized (&amp;sim;d42 and 22 before breeding) with PG-PS (30mugPG/kg/bw) or killed cells or were not immunized (Control, n = 117). IgG antibodies were detected by ELISA. Ewes were bred at synchronized estrus. All immunized and half of non-immunized ewes were challenged with PG-PS (60mugPG/kg/bw) d5 after breeding. Although proportion of ewes pregnant at d42 did not differ, probability of pregnancy decreased with total dose of PG-PS (p &lt; .05). Incidence of mastitis in mixed breed ewes in the middle and at the end of lactation did not differ. In conclusion, immunization of ewe lambs with PG-PS or killed cells of Strep. pyogenes did not improve pregnancy rate or incidence of mastitis after PG-PS challenge.","abstract_has_math":false,"creators":["Holaskova, Ida"],"institution":null,"degree_name":"MS","degree_level":"Thesis","degree_discipline":"Animal and Nutritional Sciences","degree_department":null,"school":null,"contributors":["Robert A. Dailey."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2002,"date_issued":"2002-08-01T07:00:00Z","date_published":"2002-08-01T07:00:00Z","updated_at":"2026-07-24T06:15:47Z","subjects":["Animal Physiology","Veterinary science","Animal sciences"],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["https://researchrepository.wvu.edu/etd/1519"],"render_values":[{"text":"https://researchrepository.wvu.edu/etd/1519","href":"https://researchrepository.wvu.edu/etd/1519","code":true}]}]},"links":{"outbound_url":"https://doi.org/10.33915/etd.1519","outbound_label":"DOI","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Robert A. Dailey."]},{"key":"dc:creator","label":"Author","values":["Holaskova, Ida"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.available","label":"Dc Date Available","values":["2019-01-17T08:00:00Z"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Animal and Nutritional Sciences"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["MS"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Animal Physiology","Veterinary science","Animal sciences"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://doi.org/10.33915/etd.1519","https://researchrepository.wvu.edu/etd/1519"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Bacterial infections associated with mastitis reduce pregnancies in cattle. Effects on pregnancy and incidence of mastitis in sheep were investigated after immunization with peptidoglycan-polysaccharide (PG-PS) and killed cells from Strep. pyogenes. Rambouillet (n = 100) and mixed breed (n = 18) ewe lambs were immunized (&sim;d42 and 22 before breeding) with PG-PS (30mugPG/kg/bw) or killed cells or were not immunized (Control, n = 117). IgG antibodies were detected by ELISA. Ewes were bred at synchronized estrus. All immunized and half of non-immunized ewes were challenged with PG-PS (60mugPG/kg/bw) d5 after breeding. Although proportion of ewes pregnant at d42 did not differ, probability of pregnancy decreased with total dose of PG-PS (p < .05). Incidence of mastitis in mixed breed ewes in the middle and at the end of lactation did not differ. In conclusion, immunization of ewe lambs with PG-PS or killed cells of Strep. pyogenes did not improve pregnancy rate or incidence of mastitis after PG-PS challenge."]},{"key":"dc:title","label":"Title","values":["Effect of peptidoglycan-polysaccharide complex on reproductive efficiency and mastitis in sheep"]}]}],"canonical_facts":{"dc:contributor":["Robert A. Dailey."],"dc:creator":["Holaskova, Ida"],"dc:date.available":["2019-01-17T08:00:00Z"],"dc:description.abstract":["Bacterial infections associated with mastitis reduce pregnancies in cattle. Effects on pregnancy and incidence of mastitis in sheep were investigated after immunization with peptidoglycan-polysaccharide (PG-PS) and killed cells from Strep. pyogenes. Rambouillet (n = 100) and mixed breed (n = 18) ewe lambs were immunized (&sim;d42 and 22 before breeding) with PG-PS (30mugPG/kg/bw) or killed cells or were not immunized (Control, n = 117). IgG antibodies were detected by ELISA. Ewes were bred at synchronized estrus. All immunized and half of non-immunized ewes were challenged with PG-PS (60mugPG/kg/bw) d5 after breeding. Although proportion of ewes pregnant at d42 did not differ, probability of pregnancy decreased with total dose of PG-PS (p < .05). Incidence of mastitis in mixed breed ewes in the middle and at the end of lactation did not differ. In conclusion, immunization of ewe lambs with PG-PS or killed cells of Strep. pyogenes did not improve pregnancy rate or incidence of mastitis after PG-PS challenge."],"dc:identifier":["https://doi.org/10.33915/etd.1519","https://researchrepository.wvu.edu/etd/1519"],"dc:subject":["Animal Physiology","Veterinary science","Animal sciences"],"dc:title":["Effect of peptidoglycan-polysaccharide complex on reproductive efficiency and mastitis in sheep"],"thesis:degree_discipline":["Animal and Nutritional Sciences"],"thesis:degree_level":["Thesis"],"thesis:degree_name":["MS"]},"updated_at":"2026-07-24T06:15:47Z"}