{"id":{"repo_id":"wvu","oai_identifier":"oai:researchrepository.wvu.edu:etd-1995"},"canonical_url":"https://search.dev.ndltd.org/etd/wvu/oai:researchrepository.wvu.edu:etd-1995","repository":{"repo_id":"wvu","name":"West Virginia University","base_url":"https://researchrepository.wvu.edu/do/oai/"},"display":{"title":"Associations of egg production with the major histocompatibility complex in broiler breeder hens","abstract":"This study documents effects of the major histocompatibility complex (MHC) on reproductive traits in a flock of Cobb X Cobb broiler breeder hens. The objective was to determine if the MHC affects reproduction of non-congenic broiler breeder hens. Genomic DNA was isolated from 35 hens. Restriction fragment length polymorphism (RFLP) analysis was performed on each sample, using a chicken class II MHC probe (Xu et al. 1989). Restriction endonuclease digestion using PvuII resulted in polymorphisms in all birds. Five polymorphic bands were identified. The largest bands, 7.5 kb and 5.4 kb, were present in one and four hens respectively. The 4.25 kb and 3.4 kb bands were present in 34 and 35 of the hens. The fifth band, 2.4 kb, was observed in only 13 of the animals. Five putative genotypes were observed, based on the combination of RFLP pattern observed. The two most frequent putative genotypes differed only in the absence (A, n = 17) or presence (B; n = 13) of the fifth band. Egg production records for these two groups were subjected to statistical analyses. Total eggs produced and number of eggs produced during a 45-week fertility study were both significantly higher for hens with genotype A. Hens with genotype B had longer pauses between ovipositions. This study provides evidence, in a non-congenic population of broiler breeder hens, that there are associations of egg production with the MHC.","abstract_html":"This study documents effects of the major histocompatibility complex (MHC) on reproductive traits in a flock of Cobb X Cobb broiler breeder hens. The objective was to determine if the MHC affects reproduction of non-congenic broiler breeder hens. Genomic DNA was isolated from 35 hens. Restriction fragment length polymorphism (RFLP) analysis was performed on each sample, using a chicken class II MHC probe (Xu et al. 1989). Restriction endonuclease digestion using PvuII resulted in polymorphisms in all birds. Five polymorphic bands were identified. The largest bands, 7.5 kb and 5.4 kb, were present in one and four hens respectively. The 4.25 kb and 3.4 kb bands were present in 34 and 35 of the hens. The fifth band, 2.4 kb, was observed in only 13 of the animals. Five putative genotypes were observed, based on the combination of RFLP pattern observed. The two most frequent putative genotypes differed only in the absence (A, n = 17) or presence (B; n = 13) of the fifth band. Egg production records for these two groups were subjected to statistical analyses. Total eggs produced and number of eggs produced during a 45-week fertility study were both significantly higher for hens with genotype A. Hens with genotype B had longer pauses between ovipositions. This study provides evidence, in a non-congenic population of broiler breeder hens, that there are associations of egg production with the MHC.","abstract_has_math":false,"creators":["Tarleton, Becky Jean"],"institution":null,"degree_name":"MS","degree_level":"Thesis","degree_discipline":"Animal and Nutritional Sciences","degree_department":null,"school":null,"contributors":["Robert A. Dailey."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":1999,"date_issued":"1999-08-01T07:00:00Z","date_published":"1999-08-01T07:00:00Z","updated_at":"2026-07-24T06:15:08Z","subjects":["Animal sciences","Animal Physiology"],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["https://researchrepository.wvu.edu/etd/992"],"render_values":[{"text":"https://researchrepository.wvu.edu/etd/992","href":"https://researchrepository.wvu.edu/etd/992","code":true}]}]},"links":{"outbound_url":"https://doi.org/10.33915/etd.992","outbound_label":"DOI","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Robert A. Dailey."]},{"key":"dc:creator","label":"Author","values":["Tarleton, Becky Jean"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.available","label":"Dc Date Available","values":["2019-01-17T08:00:00Z"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Animal and Nutritional Sciences"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["MS"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Animal sciences","Animal Physiology"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://doi.org/10.33915/etd.992","https://researchrepository.wvu.edu/etd/992"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["This study documents effects of the major histocompatibility complex (MHC) on reproductive traits in a flock of Cobb X Cobb broiler breeder hens. The objective was to determine if the MHC affects reproduction of non-congenic broiler breeder hens. Genomic DNA was isolated from 35 hens. Restriction fragment length polymorphism (RFLP) analysis was performed on each sample, using a chicken class II MHC probe (Xu et al. 1989). Restriction endonuclease digestion using PvuII resulted in polymorphisms in all birds. Five polymorphic bands were identified. The largest bands, 7.5 kb and 5.4 kb, were present in one and four hens respectively. The 4.25 kb and 3.4 kb bands were present in 34 and 35 of the hens. The fifth band, 2.4 kb, was observed in only 13 of the animals. Five putative genotypes were observed, based on the combination of RFLP pattern observed. The two most frequent putative genotypes differed only in the absence (A, n = 17) or presence (B; n = 13) of the fifth band. Egg production records for these two groups were subjected to statistical analyses. Total eggs produced and number of eggs produced during a 45-week fertility study were both significantly higher for hens with genotype A. Hens with genotype B had longer pauses between ovipositions. This study provides evidence, in a non-congenic population of broiler breeder hens, that there are associations of egg production with the MHC."]},{"key":"dc:title","label":"Title","values":["Associations of egg production with the major histocompatibility complex in broiler breeder hens"]}]}],"canonical_facts":{"dc:contributor":["Robert A. Dailey."],"dc:creator":["Tarleton, Becky Jean"],"dc:date.available":["2019-01-17T08:00:00Z"],"dc:description.abstract":["This study documents effects of the major histocompatibility complex (MHC) on reproductive traits in a flock of Cobb X Cobb broiler breeder hens. The objective was to determine if the MHC affects reproduction of non-congenic broiler breeder hens. Genomic DNA was isolated from 35 hens. Restriction fragment length polymorphism (RFLP) analysis was performed on each sample, using a chicken class II MHC probe (Xu et al. 1989). Restriction endonuclease digestion using PvuII resulted in polymorphisms in all birds. Five polymorphic bands were identified. The largest bands, 7.5 kb and 5.4 kb, were present in one and four hens respectively. The 4.25 kb and 3.4 kb bands were present in 34 and 35 of the hens. The fifth band, 2.4 kb, was observed in only 13 of the animals. Five putative genotypes were observed, based on the combination of RFLP pattern observed. The two most frequent putative genotypes differed only in the absence (A, n = 17) or presence (B; n = 13) of the fifth band. Egg production records for these two groups were subjected to statistical analyses. Total eggs produced and number of eggs produced during a 45-week fertility study were both significantly higher for hens with genotype A. Hens with genotype B had longer pauses between ovipositions. This study provides evidence, in a non-congenic population of broiler breeder hens, that there are associations of egg production with the MHC."],"dc:identifier":["https://doi.org/10.33915/etd.992","https://researchrepository.wvu.edu/etd/992"],"dc:subject":["Animal sciences","Animal Physiology"],"dc:title":["Associations of egg production with the major histocompatibility complex in broiler breeder hens"],"thesis:degree_discipline":["Animal and Nutritional Sciences"],"thesis:degree_level":["Thesis"],"thesis:degree_name":["MS"]},"updated_at":"2026-07-24T06:15:08Z"}