{"id":{"repo_id":"wvu","oai_identifier":"oai:researchrepository.wvu.edu:etd-1499"},"canonical_url":"https://search.dev.ndltd.org/etd/wvu/oai:researchrepository.wvu.edu:etd-1499","repository":{"repo_id":"wvu","name":"West Virginia University","base_url":"https://researchrepository.wvu.edu/do/oai/"},"display":{"title":"Examining physiological roles of adipose derived VEGF: Consequences in substrate availability and endurance exercise capacity in adipocyte specific VEGF deficient mice","abstract":"Reducing vascular endothelial growth factor (VEGF) in adipose tissue alters adipose vascularity and metabolic homeostasis. We hypothesized that this would also affect metabolic responses during exercise-induced stress, and that adipocyte-specific VEGF deficient (adipoVEGF-/-) mice would have impaired endurance capacity. Endurance exercise capacity in adipoVEGF-/- (n=10) and littermate control (n=11) mice was evaluated every 4 weeks between 6 & 24 weeks of age using a submaximal endurance run to exhaustion at 20 m/min, 10-degree incline. Maximal running speed, using incremental increases in speed at 30-second intervals, was tested at 25 weeks of age. Beginning at 6 weeks, and continuing with all time points, endurance run time to exhaustion was 30% lower in adipoVEGF-/- compared to controls (p<0.001). The age-associated rate of decline in endurance capacity was similar in adipoVEGF-/- vs. control mice and there was no difference in maximal running speed between the groups. Following 1 hour of running at 50% maximum running speed, adipoVEGF-/- mice displayed decreased circulating insulin, (p<0.001), glycerol (p<0.05), and a tendency for decreased glucose (p=0.06) compared to controls. These data suggest that deficits in adipose tissue vasculature are mediated by adipose VEGF and that deficiency of VEGF blunts the availability of lipid-derived substrates during endurance exercise and affects insulin secretion and glucose metabolism.","abstract_html":"Reducing vascular endothelial growth factor (VEGF) in adipose tissue alters adipose vascularity and metabolic homeostasis. We hypothesized that this would also affect metabolic responses during exercise-induced stress, and that adipocyte-specific VEGF deficient (adipoVEGF-/-) mice would have impaired endurance capacity. Endurance exercise capacity in adipoVEGF-/- (n=10) and littermate control (n=11) mice was evaluated every 4 weeks between 6 &amp; 24 weeks of age using a submaximal endurance run to exhaustion at 20 m/min, 10-degree incline. Maximal running speed, using incremental increases in speed at 30-second intervals, was tested at 25 weeks of age. Beginning at 6 weeks, and continuing with all time points, endurance run time to exhaustion was 30% lower in adipoVEGF-/- compared to controls (p&lt;0.001). The age-associated rate of decline in endurance capacity was similar in adipoVEGF-/- vs. control mice and there was no difference in maximal running speed between the groups. Following 1 hour of running at 50% maximum running speed, adipoVEGF-/- mice displayed decreased circulating insulin, (p&lt;0.001), glycerol (p&lt;0.05), and a tendency for decreased glucose (p=0.06) compared to controls. These data suggest that deficits in adipose tissue vasculature are mediated by adipose VEGF and that deficiency of VEGF blunts the availability of lipid-derived substrates during endurance exercise and affects insulin secretion and glucose metabolism.","abstract_has_math":false,"creators":["Zachwieja, Nicole J."],"institution":null,"degree_name":"MS","degree_level":"Thesis","degree_discipline":"Exercise Physiology","degree_department":null,"school":null,"contributors":["Mark Olfert","Randall Bryner","Linda Vona-Davis"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2014,"date_issued":"2014-08-01T07:00:00Z","date_published":"2014-08-01T07:00:00Z","updated_at":"2026-07-24T06:14:31Z","subjects":["Physiology","Kinesiology"],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["https://researchrepository.wvu.edu/etd/496"],"render_values":[{"text":"https://researchrepository.wvu.edu/etd/496","href":"https://researchrepository.wvu.edu/etd/496","code":true}]}]},"links":{"outbound_url":"https://doi.org/10.33915/etd.496","outbound_label":"DOI","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Mark Olfert","Randall Bryner","Linda Vona-Davis"]},{"key":"dc:creator","label":"Author","values":["Zachwieja, Nicole J."]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.available","label":"Dc Date Available","values":["2019-01-17T08:00:00Z"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Exercise Physiology"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["MS"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Physiology","Kinesiology"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://doi.org/10.33915/etd.496","https://researchrepository.wvu.edu/etd/496"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Reducing vascular endothelial growth factor (VEGF) in adipose tissue alters adipose vascularity and metabolic homeostasis. We hypothesized that this would also affect metabolic responses during exercise-induced stress, and that adipocyte-specific VEGF deficient (adipoVEGF-/-) mice would have impaired endurance capacity. Endurance exercise capacity in adipoVEGF-/- (n=10) and littermate control (n=11) mice was evaluated every 4 weeks between 6 & 24 weeks of age using a submaximal endurance run to exhaustion at 20 m/min, 10-degree incline. Maximal running speed, using incremental increases in speed at 30-second intervals, was tested at 25 weeks of age. Beginning at 6 weeks, and continuing with all time points, endurance run time to exhaustion was 30% lower in adipoVEGF-/- compared to controls (p<0.001). The age-associated rate of decline in endurance capacity was similar in adipoVEGF-/- vs. control mice and there was no difference in maximal running speed between the groups. Following 1 hour of running at 50% maximum running speed, adipoVEGF-/- mice displayed decreased circulating insulin, (p<0.001), glycerol (p<0.05), and a tendency for decreased glucose (p=0.06) compared to controls. These data suggest that deficits in adipose tissue vasculature are mediated by adipose VEGF and that deficiency of VEGF blunts the availability of lipid-derived substrates during endurance exercise and affects insulin secretion and glucose metabolism."]},{"key":"dc:title","label":"Title","values":["Examining physiological roles of adipose derived VEGF: Consequences in substrate availability and endurance exercise capacity in adipocyte specific VEGF deficient mice"]}]}],"canonical_facts":{"dc:contributor":["Mark Olfert","Randall Bryner","Linda Vona-Davis"],"dc:creator":["Zachwieja, Nicole J."],"dc:date.available":["2019-01-17T08:00:00Z"],"dc:description.abstract":["Reducing vascular endothelial growth factor (VEGF) in adipose tissue alters adipose vascularity and metabolic homeostasis. We hypothesized that this would also affect metabolic responses during exercise-induced stress, and that adipocyte-specific VEGF deficient (adipoVEGF-/-) mice would have impaired endurance capacity. Endurance exercise capacity in adipoVEGF-/- (n=10) and littermate control (n=11) mice was evaluated every 4 weeks between 6 & 24 weeks of age using a submaximal endurance run to exhaustion at 20 m/min, 10-degree incline. Maximal running speed, using incremental increases in speed at 30-second intervals, was tested at 25 weeks of age. Beginning at 6 weeks, and continuing with all time points, endurance run time to exhaustion was 30% lower in adipoVEGF-/- compared to controls (p<0.001). The age-associated rate of decline in endurance capacity was similar in adipoVEGF-/- vs. control mice and there was no difference in maximal running speed between the groups. Following 1 hour of running at 50% maximum running speed, adipoVEGF-/- mice displayed decreased circulating insulin, (p<0.001), glycerol (p<0.05), and a tendency for decreased glucose (p=0.06) compared to controls. These data suggest that deficits in adipose tissue vasculature are mediated by adipose VEGF and that deficiency of VEGF blunts the availability of lipid-derived substrates during endurance exercise and affects insulin secretion and glucose metabolism."],"dc:identifier":["https://doi.org/10.33915/etd.496","https://researchrepository.wvu.edu/etd/496"],"dc:subject":["Physiology","Kinesiology"],"dc:title":["Examining physiological roles of adipose derived VEGF: Consequences in substrate availability and endurance exercise capacity in adipocyte specific VEGF deficient mice"],"thesis:degree_discipline":["Exercise Physiology"],"thesis:degree_level":["Thesis"],"thesis:degree_name":["MS"]},"updated_at":"2026-07-24T06:14:31Z"}